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Full-Text Articles in Life Sciences

The Effect Of Curcumin (Curcuma Longa) On Biofilm Formation And Surface Proteins Of Listeria Monocytogenes, Songsirin Ruengvisesh Jan 2012

The Effect Of Curcumin (Curcuma Longa) On Biofilm Formation And Surface Proteins Of Listeria Monocytogenes, Songsirin Ruengvisesh

Masters Theses 1911 - February 2014

The food-borne pathogen Listeria monocytogenes can attach to the environmental surfaces and develop biofilm which can cause food contamination in the food industries. Sortase A and surface proteins are involved in biofilm and virulence of L. monocytogenes. Curcumin was reported to inhibit sortase A and biofilm in gram positive bacteria. The overall objective of this study was to observe the effect of curcumin (Curcuma longa) on the biofilm formation and surface proteins of L. monocytogenes.

The antibiofilm effect of curcumin against the strain LM21 (wild type) and s22-11G (sortase A defective mutant) was studied using the microtiter plate assay. No ...


Accessing The Bioavailability Of Phytochemicals In Caco-2 Cell Model And Developing A Sensitive Method For The Detection And Quantification Of These Compounds, Hana Shatara Sobers Jan 2012

Accessing The Bioavailability Of Phytochemicals In Caco-2 Cell Model And Developing A Sensitive Method For The Detection And Quantification Of These Compounds, Hana Shatara Sobers

Masters Theses 1911 - February 2014

Numerous studies have found certain unmethylated phytochemicals to possess anti-carcinogenic activity; however, they have been associated with poor oral bioavailability which is a major limiting factor in their usage in chemopreventative treatment. The purpose of this study was to investigate if methylation of a compound would affect bioavailability, in terms of transport and permeability, in a Caco-2 cell model as well as the effect of cell viability and cellular uptake in human colon cancer cell lines. Furthermore, a new analytic method using reversed-phase high performance liquid chromatography coupled with electrochemical detector (HPLC-EC) for the detection and quantification of resveratrol and ...


Purification And Characterization Of Novel Nucleases From A Thermophilic Fungus, Kyle S. Landry Jan 2012

Purification And Characterization Of Novel Nucleases From A Thermophilic Fungus, Kyle S. Landry

Masters Theses 1911 - February 2014

A thermophilic fungus was isolated from composted horse manure. The organism was as a Chaetomium sp. by sequencing the highly conserved ITS region of the fungus and comparing to known regions in a genomic database and was referred to as TM-417. TM-417 was found to have an optimal growth temperature of 45 oC and an optimal pH of 7.0. An extracellular DNase and RNase was found to be produced by the isolate and were purified 145.58-fold and 127.6-fold respectively using a combination of size exclusion chromatography and a novel affinity membrane purification system. The extent of ...