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Full-Text Articles in Life Sciences

Fabrication, Characterization And Utilization Of Filled Hydrogel Particles As Food Grade Delivery Systems, Alison M. Matalanis Sep 2012

Fabrication, Characterization And Utilization Of Filled Hydrogel Particles As Food Grade Delivery Systems, Alison M. Matalanis

Open Access Dissertations

Filled hydrogel particles consisting of emulsified oil droplets encapsulated within a hydrogel matrix were fabricated based on the phase separation of proteins and polysaccharides through aggregative and segregative mechanisms. A 3% (wt/wt) pectin and 3% (wt/wt) caseinate mixture at pH 7 separated into an upper pectin-rich phase and a lower casein-rich phase. Casein-coated lipid droplets added to this mixture partitioned into the lower casein-rich phase. When shear was applied, an oil-in-water-in-water (O/W1/W2) emulsion consisting of oil droplets (O) contained within a casein-rich dispersed phase (W1) suspended in a pectin-rich continuous phase (W2) was formed. Acidification from ...


Strategies To Improve The Performance Of Antioxidants In Oil-In-Water Emulsions, Atikorn Panya Sep 2012

Strategies To Improve The Performance Of Antioxidants In Oil-In-Water Emulsions, Atikorn Panya

Open Access Dissertations

Due to the limited number of approved antioxidants for food applications, several alternative strategies to improve antioxidant performance have been developed by focusing on synergistic antioxidant interactions. Susceptibility to lipid oxidation in food systems is the result of the summation of antioxidative and prooxidative mechanisms. Understanding the sometimes paradoxical behavior of antioxidants and prooxidants is a vital key to design synergistic antioxidant systems suitable for particular foods. This research focused on 3 main strategies to improve the performance of antioxidant activity in oil-in-water emulsions.

The first part of this research has been focused on inhibition of lipid oxidation by a ...


Adsorption Column Studies To Predict The Flow Of Nutrients Through Heterogenous Porous Media Under Equilibrium And Isothermal Conditions, Vijai B. Pandey Sep 2012

Adsorption Column Studies To Predict The Flow Of Nutrients Through Heterogenous Porous Media Under Equilibrium And Isothermal Conditions, Vijai B. Pandey

Open Access Dissertations

Because of the endangerment of life of the human beings due to the environmental pollution, a serious study of the pollution of the environment is most vital. Because of increasing surface water pollution there is great hazard of ground water pollution. About half the United States of America derives drinking water from aquifers and much of the projected demand is expected to be met from subsurface sources. Therefore, a study was needed to determine the process of ground water contamination due to nutrients, especially Nitrogen, its degree and Length and Time of Travel and factors inhibiting its flow through soil ...


Minor Components And Their Roles On Lipid Oxidation In Bulk Oil That Contains Association Colloids, Bingcan Chen May 2012

Minor Components And Their Roles On Lipid Oxidation In Bulk Oil That Contains Association Colloids, Bingcan Chen

Open Access Dissertations

The combination of water and surface active compounds found naturally in commercially refined vegetable oils have been postulated to form physical structures known as association colloids. This research studied the ability of 1,2-dioleoyl-sn-glycerol-3-phosphocholine (DOPC) and water to form physical structures in stripped soybean oil. Interfacial tension and fluorescence spectrometry results showed the critical micelle concentration (CMC) of DOPC in stripped soybean oil was 650 and 950 microM, respectively. Light scattering attenuation results indicated that the structure formed by DOPC was reverse micelles. The physical properties of DOPC reverse micelles were determined using small-angle X-ray scattering (SAXS) and fluorescence probes ...


The Effect Of Curcumin (Curcuma Longa) On Biofilm Formation And Surface Proteins Of Listeria Monocytogenes, Songsirin Ruengvisesh Jan 2012

The Effect Of Curcumin (Curcuma Longa) On Biofilm Formation And Surface Proteins Of Listeria Monocytogenes, Songsirin Ruengvisesh

Masters Theses 1911 - February 2014

The food-borne pathogen Listeria monocytogenes can attach to the environmental surfaces and develop biofilm which can cause food contamination in the food industries. Sortase A and surface proteins are involved in biofilm and virulence of L. monocytogenes. Curcumin was reported to inhibit sortase A and biofilm in gram positive bacteria. The overall objective of this study was to observe the effect of curcumin (Curcuma longa) on the biofilm formation and surface proteins of L. monocytogenes.

The antibiofilm effect of curcumin against the strain LM21 (wild type) and s22-11G (sortase A defective mutant) was studied using the microtiter plate assay. No ...


Accessing The Bioavailability Of Phytochemicals In Caco-2 Cell Model And Developing A Sensitive Method For The Detection And Quantification Of These Compounds, Hana Shatara Sobers Jan 2012

Accessing The Bioavailability Of Phytochemicals In Caco-2 Cell Model And Developing A Sensitive Method For The Detection And Quantification Of These Compounds, Hana Shatara Sobers

Masters Theses 1911 - February 2014

Numerous studies have found certain unmethylated phytochemicals to possess anti-carcinogenic activity; however, they have been associated with poor oral bioavailability which is a major limiting factor in their usage in chemopreventative treatment. The purpose of this study was to investigate if methylation of a compound would affect bioavailability, in terms of transport and permeability, in a Caco-2 cell model as well as the effect of cell viability and cellular uptake in human colon cancer cell lines. Furthermore, a new analytic method using reversed-phase high performance liquid chromatography coupled with electrochemical detector (HPLC-EC) for the detection and quantification of resveratrol and ...


Purification And Characterization Of Novel Nucleases From A Thermophilic Fungus, Kyle S. Landry Jan 2012

Purification And Characterization Of Novel Nucleases From A Thermophilic Fungus, Kyle S. Landry

Masters Theses 1911 - February 2014

A thermophilic fungus was isolated from composted horse manure. The organism was as a Chaetomium sp. by sequencing the highly conserved ITS region of the fungus and comparing to known regions in a genomic database and was referred to as TM-417. TM-417 was found to have an optimal growth temperature of 45 oC and an optimal pH of 7.0. An extracellular DNase and RNase was found to be produced by the isolate and were purified 145.58-fold and 127.6-fold respectively using a combination of size exclusion chromatography and a novel affinity membrane purification system. The extent of ...