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Food Science

2010

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Articles 31 - 60 of 245

Full-Text Articles in Life Sciences

4-H Fun With Foods - Lesson 7: Sandwiches, Georgia C. Lauritzen, Darlene Christensen, Susan Haws, Ronda Olsen, Naomi Weeks, Debra Proctor Dec 2010

4-H Fun With Foods - Lesson 7: Sandwiches, Georgia C. Lauritzen, Darlene Christensen, Susan Haws, Ronda Olsen, Naomi Weeks, Debra Proctor

All Current Publications

This publication is part of the 4-H Fun with Foods Guide, Lesson 7 provides recipes and instructions for making a variety of sandwiches.


Dutch Oven Cooking, Susan Haws Dec 2010

Dutch Oven Cooking, Susan Haws

All Current Publications

This is a how-to publication on dutch oven cooking details.


Controlling The Mineral Content Of Sweet Whey Powder In An Industrial Setting, Matthew W. Sawyer Dec 2010

Controlling The Mineral Content Of Sweet Whey Powder In An Industrial Setting, Matthew W. Sawyer

Dairy Science

The objective of this project was to reduce the final ash content of sweet whey powder. The data for this project was collected over a two-week period, from July 13th to July 27th 2010 in a dairy plant in the Mid-West of the United States. The data is composed of particle size test, ash test, and pH test. The conclusions of this study are that multiple pre-heat treatment tank changes contribute to mineral content by requiring more buffering than usual. The low pH of this pre-heat liquid forces a need for more buffering. Particle segregation may play a role in ...


Antimicrobial Activity Of Casein Hydrolysates Against Listeria Monocytogenes And Escherichia Coli O157:H7, Jessica M Christman Dec 2010

Antimicrobial Activity Of Casein Hydrolysates Against Listeria Monocytogenes And Escherichia Coli O157:H7, Jessica M Christman

Masters Theses

Listeriosis has the highest fatality and hospitalization rate among foodborne illnesses. Listeria monocytogenes causes listeriosis and is a difficult bacterium for ready-to-eat food processors to eliminate because of its ability to grow in the absence of oxygen and under refrigeration. Recently, milk and its proteins have gained recognition as the largest source of biologically active peptides, and, it stands reason that several antimicrobial peptides (AMP) can be released from casein as it is the most abundant milk protein. AMPs are commonly obtained by cutting the whole protein into peptide fragments using enzymes or by acidification. The objective of this study ...


Novel Approaches For The Efficient Sampling And Detection Of Listeria Monocytogenes And Brochothrix Thermosphacta On Food Contact Surfaces, Jessica Anne Clemons Dec 2010

Novel Approaches For The Efficient Sampling And Detection Of Listeria Monocytogenes And Brochothrix Thermosphacta On Food Contact Surfaces, Jessica Anne Clemons

Masters Theses

The primary step in the microbiological assessment of highly dynamic and complex food processing conditions is environmental sampling. The objectives of this study were to: (1) compare the efficacy of four sampling devices including Microbial-Vac system (MV), cellulose sponge (SP), polyester swab (SW) and composite tissue (CT), for the recovery of Listeria monocytogenes and Brochothrix thermosphacta on five surfaces and (2) to determine if there was a significant difference between the recovery of low (10 CFU/900cm2) and high (100 CFU/900cm2) L. monocytogenes inoculum levels using the sampling devices in a simulated food processing environment. Surfaces used ...


4-H Fun With Foods - Lesson 8: Plan A Party, Georgia C. Lauritzen, Darlene Christensen, Susan Haws, Naomi Weeks, Debra Proctor, Ronda Olsen Dec 2010

4-H Fun With Foods - Lesson 8: Plan A Party, Georgia C. Lauritzen, Darlene Christensen, Susan Haws, Naomi Weeks, Debra Proctor, Ronda Olsen

All Current Publications

This publication is part of the 4-H Fun with Food Guide, Lesson 8 provides ideas, instructions, checklists, and recipes for planning a party.


4-H Fun With Foods - Lessons 9 And 10: Smart Shopping, Have A Party, Georgia C. Lauritzen, Darlene Christensen, Susan Haws, Debra Proctor, Ronda Olsen, Naomi Weeks Dec 2010

4-H Fun With Foods - Lessons 9 And 10: Smart Shopping, Have A Party, Georgia C. Lauritzen, Darlene Christensen, Susan Haws, Debra Proctor, Ronda Olsen, Naomi Weeks

All Current Publications

This publication is part of the 4-H Fun with Food Guide, Lesson 9 and 10 provide smart tips for grocery shopping and executing party plans.


Chemical Composition And Antioxidant Properties Of Maya Nut (Brosimum Alicastrum), Hatice Kubra Tokpunar Dec 2010

Chemical Composition And Antioxidant Properties Of Maya Nut (Brosimum Alicastrum), Hatice Kubra Tokpunar

All Theses

ABSTRACT
Maya nut was investigated in term of its moisture, ash content, antioxidant activities, phenolic components by high pressure liquid chromatography-ultraviolet detector (HPLC-UV) and fatty acid composition by gas chromatography-flame ionization detector (GC-FID). The results showed that Maya nut was mainly composed of 55.06% linoleic acid (C18:2 n6), 27.2% palmitic acid (C16:0), 6.26% stearic acid (C18:0), 9.9% linolenic acid (C18:3 n3), and 3.36% Cis-11-Eicosenoic (C20:1). Maya nut possesses a higher content of polyunsaturated fatty acids (PUFAs) (linoleic and linolenic acid), but lower content of monounsaturated fatty acids (MUFAs) than canola ...


Shelf Life Studies On Processed Peaches, Divija Unhale Dec 2010

Shelf Life Studies On Processed Peaches, Divija Unhale

All Theses

A series of experiments were conducted on the preservation of fresh peaches using freezing and heat (hot-filled packaging). In the first experiment, the effects of four calcium salts (calcium chloride, calcium lactate, calcium citrate, calcium phosphate) on the texture of processed peach slices were determined. Peach slices were immersed in solutions containing dissolved calcium salts at levels of 0.5, 1 and 2 % for 1 minute then stored at 4 ¡C for 3 days. Treatments with calcium salts were firmer than non calcium treated peach slices at all levels. Calcium lactate at 2 % level was best in terms of texture ...


Effect Of Crust Freezing On The Quality And Survival Of Escherichia Coli And Salmonella Typhimurium In Raw Poultry Products, Byron Chaves-Elizondo Dec 2010

Effect Of Crust Freezing On The Quality And Survival Of Escherichia Coli And Salmonella Typhimurium In Raw Poultry Products, Byron Chaves-Elizondo

All Theses

Industry has widely used freezing as a strategy to halt pathogen growth and more recently, crust freezing has been suggested as a means to improve mechanical operations, quality, and safety of poultry products. Two separate studies were conducted to evaluate the effect of crust freezing (20 min, -85 ¡C) on the survival of Escherichia coli and Salmonella Typhimurium and on quality and shelf life of raw chicken breasts, with or without skin. For the first study, ampicillin-resistant E. coli JM 109 and nalidixic acid-resistant S. Typhimurium were used in the experiments. A set of cultures was subjected to cold-shock stress ...


Isolation, Characterization And Application Of Bacteriophage To Treat Hydrogen Sulfide Producing Bacteria In Raw Animal Materials Destined For The Rendering Process, Chao Gong Dec 2010

Isolation, Characterization And Application Of Bacteriophage To Treat Hydrogen Sulfide Producing Bacteria In Raw Animal Materials Destined For The Rendering Process, Chao Gong

All Theses

In the United States, billions of pounds of animal by-products are generated by the food processing industry every year. Hydrogen sulfide producing bacteria (SPB) can utilize the sulfur-containing proteins and amino acids in the raw animal materials destined for the rendering process to produce harmful hydrogen sulfide (H2S) gas rapidly under the ambient conditions, resulting in hazardous working environments and inferior quality of finished products. In this study, the application of bacteriophage was explored as an effective solution for the elimination of H2S production in the rendering industry. The objectives of this study were to: 1) isolate and characterize strains ...


Regulatory Issues And Functional Health Claims For Bioactive Dairy Compounds, P. Roupas, P. G. Williams, C. Margetts Nov 2010

Regulatory Issues And Functional Health Claims For Bioactive Dairy Compounds, P. Roupas, P. G. Williams, C. Margetts

Peter Williams

Dairy foods and ingredients have a natural advantage over new/novel foods, from a regulatory viewpoint, because they are generally considered as “traditional” foods, that is, there is a long history of human consumption. However, the regulatory landscape on adding bioactive ingredients, whether from dairy streams or from non-dairy sources, into dairy foods is rapidly evolving, and the dairy industry will need to be aware of potential regulatory challenges, within the countries they wish to market their products.


Conditions Associated With Clostridium Sporogenes Growth As A Surrogate For Clostridium Botulinum In Non-Thermally Processed Canned Butter, Reed H. Taylor Nov 2010

Conditions Associated With Clostridium Sporogenes Growth As A Surrogate For Clostridium Botulinum In Non-Thermally Processed Canned Butter, Reed H. Taylor

Theses and Dissertations

Shelf-stable canned butter is currently available in retail stores, and many home-preservationists promote home-canning of butter. Non-cultured butter is a low-acid canned food, which would presumably require thermal processing. The lack of a thermal process step in canned butter products raises questions of potential safety, because they are hermetically sealed and generally exhibit anaerobic growth conditions, which are optimal for Clostridium botulinum growth. Without thermal processing, low-acid canned foods (LACF) must have inhibitory factors present to prevent C. botulinum growth. Some potential intrinsic inhibitory factors, or "hurdles", within butter include: reduced water activity (aw), acidity (pH) in cultured products, elevated ...


Androidal Fat Dominates In Predicting Cardiometabolic Risk In Postmenopausalwomen, O. A. Matvienko, D. L. Alekel, S. N. Bhupathiraju, Heike Hofmann, L. M. Ritland, M. B. Reddy, M. D. Van Loan, C. D. Perry Nov 2010

Androidal Fat Dominates In Predicting Cardiometabolic Risk In Postmenopausalwomen, O. A. Matvienko, D. L. Alekel, S. N. Bhupathiraju, Heike Hofmann, L. M. Ritland, M. B. Reddy, M. D. Van Loan, C. D. Perry

Food Science and Human Nutrition Publications

We hypothesized that soy isoflavones would attenuate the anticipated increase in androidal fat mass in postmenopausal women during the 36-month treatment, and thereby favorably modify the circulating cardiometabolic risk factors: triacylglycerol, LDLC, HDL-C, glucose, insulin, uric acid, C-reactive protein, fibrinogen, and homocysteine. We collected data on 224 healthy postmenopausal women at risk for osteoporosis (45.8–65 y, median BMI 24.5) who consumed placebo or soy isoflavones (80 or 120 mg/d) for 36 months and used longitudinal analysis to examine the contribution of isoflavone treatment, androidal fat mass, other biologic factors, and dietary quality to cardiometabolic outcomes. Except ...


The Influence Of Persistent Pathogens On Circulating Levels Of Inflammatory Markers: A Cross-Sectional Analysis From The Multi-Ethnic Study Of Atherosclerosis, Aydin Nazmi, Ana V. Diez-Roux, Nancy S. Jenny, Michael Y. Tsai, Moyses Szklo, Allison E. Aiello Nov 2010

The Influence Of Persistent Pathogens On Circulating Levels Of Inflammatory Markers: A Cross-Sectional Analysis From The Multi-Ethnic Study Of Atherosclerosis, Aydin Nazmi, Ana V. Diez-Roux, Nancy S. Jenny, Michael Y. Tsai, Moyses Szklo, Allison E. Aiello

Food Science and Nutrition

Background: Systemic inflammation is linked to cardiovascular risk, but the influence of persistent pathogens, which are conventionally dichotomously categorized, on circulating levels of inflammatory markers is not clear. Antibody levels of pathogens have not been examined in relation to inflammation.
Methods: Using data from a subsample of the Multi-Ethnic Study of Atherosclerosis, we examined circulating levels of interleukin-6 (IL-6), C-reactive protein (CRP) and fibrinogen in relation to five common persistent pathogens: cytomegalovirus, herpes simplex virus-1, Hepatitis A virus, Helicobacter pylori and Chlamydia pneumoniae. We tested the hypothesis that the number of seropositive pathogens (based on conventional cut-off points) would not ...


Accelerated Shelf Life Determination Of Antioxidant Stabilized High Oleic Sunflower And Canola Oils In Plastic Bottles, Christine Nicole Shearer Nov 2010

Accelerated Shelf Life Determination Of Antioxidant Stabilized High Oleic Sunflower And Canola Oils In Plastic Bottles, Christine Nicole Shearer

Theses and Dissertations

Vegetable oil is an important commodity; however, the majority of commercially available vegetable oils have a fairly short shelf life, which limits their usefulness for long term food storage, disaster relief, space travel, food aid programs, and military rations. Vegetable oils with high oleic acid and reduced linolenic acid contents, especially with added antioxidant combinations, were previously found to have significantly longer oil stability index (OSI) values than traditional vegetable oils. This study used accelerated shelf life testing to estimate the ambient shelf life of high oleic sunflower oil (HOSUN) and high oleic canola oil (HOCAN), each containing 1,000 ...


Deactivation Of Soybean Agglutinin By Enzymatic And Other Physical Treatments, Yating Ma, Tong Wang Nov 2010

Deactivation Of Soybean Agglutinin By Enzymatic And Other Physical Treatments, Yating Ma, Tong Wang

Food Science and Human Nutrition Publications

The main objective of this study was to eliminate the hemagglutination activity of an antinutritional factor in soybeans, soybean agglutinin (SBA). A series of experiments was designed to enzymatically modify SBA structure and to use other physical treatments to reduce activity. SBA extract was prepared from soy flour and used as the substrate for all treatments. Deglycosylation by enzyme decreased activity of SBA by 21%, but not to the level of denaturation by heat or by denaturing reagents (47−77% residual activity). Single enzymes, such as trypsin, chymotrypsin, thermolysin, and endoproteinase Glu-C, did not hydrolyze native SBA, but they hydrolyzed ...


Composition And Antimicrobial Activities Of Lippia Multiflora Moldenke, Mentha X Piperita L. And Ocimum Basilicum L. Essential Oils And Their Major Monoterpene Alcohols Alone And In Combination, Mamoudou H. Dicko Prof., Aline Aline Lamien-Meda, Balé Bayala, Souleymane Tirogo, Chlodwig Chlodwig Franz, Johannes Johannes Novak, Roger Charles Roger Charles Nebié Nov 2010

Composition And Antimicrobial Activities Of Lippia Multiflora Moldenke, Mentha X Piperita L. And Ocimum Basilicum L. Essential Oils And Their Major Monoterpene Alcohols Alone And In Combination, Mamoudou H. Dicko Prof., Aline Aline Lamien-Meda, Balé Bayala, Souleymane Tirogo, Chlodwig Chlodwig Franz, Johannes Johannes Novak, Roger Charles Roger Charles Nebié

Pr. Mamoudou H. DICKO, PhD

Essential oils from leaves of Lippia multiflora, Mentha x piperita and Ocimum basilicum from Burkina Faso were analysed by GC–FID and GC–MS. Major components were p-cymene, thymol, β-caryophyllene, carvacrol and carvone for L. multiflora, menthol and iso-menthone for M. x piperita and, linalool and eugenol for O. basilicum. The essential oils and their major monoterpene alcohols were tested against nine bacterial strains using the disc diffusion and broth microdilution methods. The essential oils with high phenolic contents were the most effective antimicrobials. The checkerboard method was used to quantify the efficacy of paired combinations of essential oils and ...


Water And Fertilizer Influence On Sorghum Grain Quality For Traditional Beer (Dolo) Production In Burkina Faso, S. Palé, S. J.B. Taonda, B. Bougouma, Stephen C. Mason Nov 2010

Water And Fertilizer Influence On Sorghum Grain Quality For Traditional Beer (Dolo) Production In Burkina Faso, S. Palé, S. J.B. Taonda, B. Bougouma, Stephen C. Mason

INTSORMIL Scientific Publications

In the Central Plateau of Burkina Faso, grain sorghum [Sorghum bicolor (L.) Moench] is the major cereal crop used to produce the traditional beer called dolo. Grain sorghum grain samples collected in 2004 and 2005 from experiments combining five water management techniques and four fertilizer treatments in a randomized complete block design with a split plot arrangement of treatments were analyzed for the physicochemical properties of raw grain, and quality of malted grain. Water management techniques were allocated to main plots and fertilizer treatments to subplots. The objective was to determine the best combination of water management technique and fertilizer ...


Identification And Characterization Of Salmonella Serotypes Isolated From Pork And Poultry From Commercial Sources, Yulie Edith Meneses-GonzáLez Nov 2010

Identification And Characterization Of Salmonella Serotypes Isolated From Pork And Poultry From Commercial Sources, Yulie Edith Meneses-GonzáLez

Dissertations, Theses, & Student Research in Food Science and Technology

Salmonella is one of the leading causes of foodborne illness worldwide, and it is estimated that 1.4 million infections occur annually in the U.S. alone. The Premi®Test Salmonella (PTS), is a potential tool for rapid detection and identification of Salmonella serovars. The objective of this project was to evaluate the use of the PTS system as a serotyping tool to identify pork and poultry isolates obtained from vertically integrated operations and to characterize their antibiotic resistance. Two hundred isolates were evaluated. All isolates were serotyped using the traditional Kaufmann-White scheme and the PTS system. Among the isolates ...


Development Of An Enzyme-Linked Immunosorbent Assay (Elisa) For The Detection Of Pistachio Residues In Processed Foods, Pei Wen Lim Nov 2010

Development Of An Enzyme-Linked Immunosorbent Assay (Elisa) For The Detection Of Pistachio Residues In Processed Foods, Pei Wen Lim

Dissertations, Theses, & Student Research in Food Science and Technology

Pistachios (Pistacia vera) are popular snacks and consumption of pistachios is notably increasing due to their usage as ingredients for confections, ice cream, and baked goods. The increased consumption of pistachios may lead to a higher frequency of allergic sensitization and an increased prevalence of allergic reactions to pistachios. Trace amounts of undeclared pistachio allergens can pose serious health risks for food-allergic consumers, including severe anaphylactic reactions. A highly sensitive analytical method, sandwich-type enzyme-linked immunosorbent assay (ELISA) is applied as a critical tool for food manufacturers and food scientists to detect minute amounts of allergenic food residues in processed foods ...


Functional And Bioactive Components From Mackerel (Scomber Scombrus) And Blue Whiting (Micromesistius Poutassou) Processing Waste, Zied Khiari Nov 2010

Functional And Bioactive Components From Mackerel (Scomber Scombrus) And Blue Whiting (Micromesistius Poutassou) Processing Waste, Zied Khiari

Doctoral

The use of fish processing waste from an oily fish model (mackerel, Scomber
scombrus) and a white fish model (blue whiting, Micromesistius poutassou) as a source of functional and bioactive compounds was investigated.
Gelatines were extracted from fish heads and skins using pre-treatment with
different organic acids (acetic, citric, lactic, tartaric and malic acids). Gelatines from fish bones were extracted after pre-treating the bones either chemically or enzymatically. The type of pre-treatment affected the yield, colour, turbidity, amino acid profiles as well as functional properties of fish gelatines. Mackerel gelatines formed stronger and more stable gels than blue whiting. Acetic ...


Diabetes And The Health Care Team, Ingrid Adams Nov 2010

Diabetes And The Health Care Team, Ingrid Adams

Family and Consumer Sciences Publications

Diabetes is a disease that affects many parts of the body, such as the eyes, kidneys, blood vessels, heart, legs and feet. As a result, a team approach to taking care of the disease can be very helpful. When a team of individuals works together problems are identified earlier, and it is easier to reduce or prevent diabetes complications.

You, your doctor and family members may be the initial members of your team. Other team members may include a registered dietitian, certified diabetes educator, diabetes nurse, pharmacist, dentist, eye doctor, foot doctor, social worker and a mental health counselor.

You ...


Monitoring Blood Glucose, Ingrid Adams Nov 2010

Monitoring Blood Glucose, Ingrid Adams

Family and Consumer Sciences Publications

Blood glucose monitoring lets you know what your blood glucose level is at the time of testing. The reading provides helpful information on how well you are managing your diabetes. Keeping your blood glucose level within the target range set by you and your doctor reduces the risk of diabetes complications. It is important to check your blood glucose regularly so you can see how certain foods, activities and medicine affect your blood glucose level.


Simple Sorghum Grain Quality Evaluation Procedures, Janet Taylor, John R.N. Taylor Nov 2010

Simple Sorghum Grain Quality Evaluation Procedures, Janet Taylor, John R.N. Taylor

INTSORMIL Presentations

Most Appropriate Sorghum Grain Quality Criteria Identified

•High tannin/non-tannin

•Hardness

•Germinability

•Grain purity

•Grain color

Tannin, hardness, germinability and grain purity have been accepted as draft ICC (International Association for Cereal Science and Technology) standard methods.


Cross-Sectional And Longitudinal Associations Of Neighborhood Characteristics With Inflammatory Markers: Findings From The Multi-Ethnic Study Of Atherosclerosis, Aydin Nazmi, Ana Diez Roux, Nalini Ranjit, Teresa E. Seeman, Nancy S. Jenny Nov 2010

Cross-Sectional And Longitudinal Associations Of Neighborhood Characteristics With Inflammatory Markers: Findings From The Multi-Ethnic Study Of Atherosclerosis, Aydin Nazmi, Ana Diez Roux, Nalini Ranjit, Teresa E. Seeman, Nancy S. Jenny

Food Science and Nutrition

We investigated cross-sectional associations of neighborhood deprivation, problems, safety, and cohesion with circulating levels of fibrinogen, interleukin-6, and C-reactive protein (n=5370) and longitudinal associations with changes in IL-6 over a 3–4 year period (n=946). In cross-sectional analyses, higher levels of neighborhood deprivation and problems were associated with higher levels of all three inflammatory markers, whereas higher levels of safety were associated with lower levels. Fibrinogen remained associated with all neighborhood characteristics except cohesion and IL-6 remained associated with safety after adjustment for race and SES. In longitudinal analyses, higher levels of neighborhood deprivation and problems, and lower ...


Enhancing Chocolate Flavour Quality – Effects Of Technological Modifications During Fermentation Of Ghanaian Cocoa Beans, Dr. Emmanuel Ohene Afoakwa (Phd) Oct 2010

Enhancing Chocolate Flavour Quality – Effects Of Technological Modifications During Fermentation Of Ghanaian Cocoa Beans, Dr. Emmanuel Ohene Afoakwa (Phd)

Professor Emmanuel Ohene Afoakwa

No abstract provided.


Can Grain Be Disinfested In 5-Gal Hdpe Buckets Containing Oxygen Absorber Packets?, Lynn V. Ogden, C. Griffin Oct 2010

Can Grain Be Disinfested In 5-Gal Hdpe Buckets Containing Oxygen Absorber Packets?, Lynn V. Ogden, C. Griffin

Faculty Publications

Effective methods of grain disinfestation include freezing, the use of carbon dioxide gas, and the use of pesticides. Oxygen deprivation has also been shown to be an effective method of disinfestation when the oxygen content is held below 1% for at least 12 days (d). High Density Polyethylene (HDPE) 5 gallon (gal) buckets have become a popular and convenient storage container for dry foods such as wheat, rice and beans. Oxygen absorber packets are an effective way to remove the oxygen in high-barrier sealed containers to prolong the life of many of these foods. It is hypothesized that sealed HDPE ...


Feasibility Of Re-Using Pete Soda Bottles To Exclude Oxygen During Storage Of Low Moisture Foods, Sarah Broderick, Michelle A. Lloyd, Lynn V. Ogden, Oscar A. Pike Oct 2010

Feasibility Of Re-Using Pete Soda Bottles To Exclude Oxygen During Storage Of Low Moisture Foods, Sarah Broderick, Michelle A. Lloyd, Lynn V. Ogden, Oscar A. Pike

Faculty Publications

Many consumers are beginning to store grain on their own creating a need for easy disinfestation methods that can be accomplished in the home. Oxygen deprivation has proven to be an effective method for grain disinfestation when kept below 1% for 12 days. Re-using PETE soda bottles is an easy way many consumers can store their grains. The purpose of this study was to determine if using oxygen absorber packets in used PETE soda bottles will keep the oxygen percent low enough to disinfest the grain stored in them. Experiments were carried out to determine how long oxygen absorber packets ...


Combination Of Adhesive-Tape-Based Sampling And Fluorescence In Situ Hybridization For Rapid Detection Of Salmonella On Fresh Produce, Bledar Bisha, Byron F. Brehm-Stecher Oct 2010

Combination Of Adhesive-Tape-Based Sampling And Fluorescence In Situ Hybridization For Rapid Detection Of Salmonella On Fresh Produce, Bledar Bisha, Byron F. Brehm-Stecher

Food Science and Human Nutrition Publications

This protocol describes a simple approach for adhesive-tape-based sampling of tomato and other fresh produce surfaces, followed by on-tape fluorescence in situ hybridization (FISH) for rapid culture-independent detection of Salmonella spp. Cell-charged tapes can also be placed facedown on selective agar for solid-phase enrichment prior to detection. Alternatively, low-volume liquid enrichments (liquid surface miniculture) can be performed on the surface of the tape in non-selective broth, followed by FISH and analysis via flow cytometry. To begin, sterile adhesive tape is brought into contact with fresh produce, gentle pressure is applied, and the tape is removed, physically extracting microbes present on ...