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Full-Text Articles in Life Sciences

Effect Of Crust Freezing On The Quality And Survival Of Escherichia Coli And Salmonella Typhimurium In Raw Poultry Products, Byron Chaves-Elizondo Dec 2010

Effect Of Crust Freezing On The Quality And Survival Of Escherichia Coli And Salmonella Typhimurium In Raw Poultry Products, Byron Chaves-Elizondo

All Theses

Industry has widely used freezing as a strategy to halt pathogen growth and more recently, crust freezing has been suggested as a means to improve mechanical operations, quality, and safety of poultry products. Two separate studies were conducted to evaluate the effect of crust freezing (20 min, -85 ¡C) on the survival of Escherichia coli and Salmonella Typhimurium and on quality and shelf life of raw chicken breasts, with or without skin. For the first study, ampicillin-resistant E. coli JM 109 and nalidixic acid-resistant S. Typhimurium were used in the experiments. A set of cultures was subjected to cold-shock stress ...


Identification And Characterization Of Salmonella Serotypes Isolated From Pork And Poultry From Commercial Sources, Yulie Edith Meneses-GonzáLez Nov 2010

Identification And Characterization Of Salmonella Serotypes Isolated From Pork And Poultry From Commercial Sources, Yulie Edith Meneses-GonzáLez

Dissertations, Theses, & Student Research in Food Science and Technology

Salmonella is one of the leading causes of foodborne illness worldwide, and it is estimated that 1.4 million infections occur annually in the U.S. alone. The Premi®Test Salmonella (PTS), is a potential tool for rapid detection and identification of Salmonella serovars. The objective of this project was to evaluate the use of the PTS system as a serotyping tool to identify pork and poultry isolates obtained from vertically integrated operations and to characterize their antibiotic resistance. Two hundred isolates were evaluated. All isolates were serotyped using the traditional Kaufmann-White scheme and the PTS system. Among the isolates ...


Combination Of Adhesive-Tape-Based Sampling And Fluorescence In Situ Hybridization For Rapid Detection Of Salmonella On Fresh Produce, Bledar Bisha, Byron F. Brehm-Stecher Oct 2010

Combination Of Adhesive-Tape-Based Sampling And Fluorescence In Situ Hybridization For Rapid Detection Of Salmonella On Fresh Produce, Bledar Bisha, Byron F. Brehm-Stecher

Food Science and Human Nutrition Publications

This protocol describes a simple approach for adhesive-tape-based sampling of tomato and other fresh produce surfaces, followed by on-tape fluorescence in situ hybridization (FISH) for rapid culture-independent detection of Salmonella spp. Cell-charged tapes can also be placed facedown on selective agar for solid-phase enrichment prior to detection. Alternatively, low-volume liquid enrichments (liquid surface miniculture) can be performed on the surface of the tape in non-selective broth, followed by FISH and analysis via flow cytometry. To begin, sterile adhesive tape is brought into contact with fresh produce, gentle pressure is applied, and the tape is removed, physically extracting microbes present on ...


The Effects Of High Pressure Processing On Peanut Sauce Inoculated With Salmonella, Tara K. Stiles Jul 2010

The Effects Of High Pressure Processing On Peanut Sauce Inoculated With Salmonella, Tara K. Stiles

Dissertations, Theses, & Student Research in Food Science and Technology

Recent Salmonella outbreaks have prompted the need for new processing options for peanut products. Traditional heating kill-steps have shown to be ineffective in lipid-rich matrices such as peanut products. High pressure processing is one such option for peanut sauce because it has a high water activity, which has proved to be a large contributing factor in microbial lethality due to high pressure processing. Four different formulations of peanut sauce were inoculated with a five strain Salmonella cocktail and high pressure processed. Results indicate that increasing pressure or increasing hold time increases log10 reductions. The Weibull model was fitted to ...


Dynamic Predictive Model For The Growth Of Salmonella Spp. In Liquid Whole Egg And Risk Evaluation Of Egg White Hydrolysates Manufacturing Process For Spore Formers: Bacillus Cereus And Clostridium Perfringens., Aikansh Singh May 2010

Dynamic Predictive Model For The Growth Of Salmonella Spp. In Liquid Whole Egg And Risk Evaluation Of Egg White Hydrolysates Manufacturing Process For Spore Formers: Bacillus Cereus And Clostridium Perfringens., Aikansh Singh

Dissertations, Theses, & Student Research in Food Science and Technology

A dynamic model for the growth of Salmonella spp. in liquid whole egg under continuously varying temperature was developed and validated. A primary model (Baranyi model) was fitted to each temperature growth data and corresponding maximum growth rates were estimated. Pseudo-R2 values were greater than 0.97 for primary models. The maximum growth rates obtained from each primary model were then plotted against temperature and modeled using the modified Ratkowsky model. The pseudo-R2 and root mean square error were 0.99 and 0.06 h-1, respectively for the secondary model. A dynamic model for the prediction of ...


An Hsi Report: Food Safety And Cage Egg Production, Humane Society International Jan 2010

An Hsi Report: Food Safety And Cage Egg Production, Humane Society International

HSI REPORTS

Governments have begun legislating against cage egg production and a growing number of major food retailers, restaurant chains, and foodservice providers worldwide are switching to cage-free eggs. Extensive scientific evidence strongly suggests this trend will improve food safety. All fifteen scientific studies published in the last five years comparing Salmonella contamination between caged and cage-free operations found that those confining hens in cages had higher rates of Salmonella, a leading cause of food poisoning worldwide. This has led prominent consumer advocacy organizations, such as the Center for Food Safety, to oppose the use of cages to confine egg-laying hens.