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Food Science

2008

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Articles 1 - 30 of 119

Full-Text Articles in Life Sciences

Oxidative Stability Of Egg And Soy Lecithin As Affected By Transition Metal Ions And Ph In Emulsion, Guang Wang, Tong Wang Dec 2008

Oxidative Stability Of Egg And Soy Lecithin As Affected By Transition Metal Ions And Ph In Emulsion, Guang Wang, Tong Wang

Food Science and Human Nutrition Publications

Oxidative stability of egg and soy lecithin in emulsion was evaluated with two transition metal ions, cupric and ferric ion, at two concentration levels (50 and 500 μM). The effect of pH on lipid oxidation was also examined under these two concentrations for each ion. Egg lecithin (EL) had similar peroxide value (PV) development pattern as soy lecithin (SL) when treated with cupric ion under both acidic and neutral pH. Acidic pH of 3 accelerated oxidation of both EL and SL, especially under high concentration of copper. When treated with ferric ion, EL oxidized much faster than SL did. EL ...


Characterization Of Dna Sequences That Confer Complement Resistance In Leishmania Chagasi, Rebecca R. Dahlin-Laborde, Eric J. Scolaro, Nathan M. Romine, Amanda Ramer-Tait, Soi Meng Lei, Jeffrey K. Beetham Dec 2008

Characterization Of Dna Sequences That Confer Complement Resistance In Leishmania Chagasi, Rebecca R. Dahlin-Laborde, Eric J. Scolaro, Nathan M. Romine, Amanda Ramer-Tait, Soi Meng Lei, Jeffrey K. Beetham

Faculty Publications in Food Science and Technology

Serial passage of axenically cultured Leishmania chagasi promastigotes results in a progressive diminution in resistance to complement-mediated lysis (CML), whereas high CML resistance is seen in infectious metacyclic promastigotes from the sandfly vector as well as metacyclic-like promastigotes within low-passage cultures at stationary growth phase. As we previously reported, in a screen seeking to identify novel genes involved in CML resistance: (1) a genomic cosmid library derived from DNA of CML-resistant L. chagasi promastigotes was transfected into highpassage (constitutively CML-sensitive) L. chagasi promastigotes; (2) transformants were screened for acquisition of CML-resistance; (3) multiple cosmid-transfectants exhibited partial CML resistance; and (4 ...


Novel Method For Recovery Of Escherichia Coli O157:H7 From Beef Surfaces, Matthew Adam Becher Dec 2008

Novel Method For Recovery Of Escherichia Coli O157:H7 From Beef Surfaces, Matthew Adam Becher

Masters Theses

A study was conducted to compare the efficiency of detection of Escherichia coli O157:H7 EDL 933 on external beef trim by commercial recovery systems based upon of the Microbial-Vac System (M-Vac) and excision sampling. Escherichia coli O157:H7 EDL 933 was cultured in tryptic soy broth for 24 hr at 37°C and transferred for three consecutive days before diluting and spot inoculating onto a 684 cm2 piece of beef trim (10 CFU/684 cm2). Beef trim was sampled with the M-Vac by passing the sampling head over the entire surface area in a vertical sampling pattern ...


“Intervention Strategies To Enhance The Safety Of Ready-To-Eat Meat Products By Plant Essential Oils, Chayapa Techathuvanan Dec 2008

“Intervention Strategies To Enhance The Safety Of Ready-To-Eat Meat Products By Plant Essential Oils, Chayapa Techathuvanan

Masters Theses

Components of plant essential oil (PEO) extracts are known to have antimicrobial properties. However, their antimicrobial efficacy in food systems is low due to their hydrophobic nature and association with other food components. Incorporation of PEO components into an appropriate carrier may offer a potential solution to improve their activity in food systems. This study was conducted to determine the effect of PEO components (thymol, eugenol, linalool, carvacrol, and cinnamaldehyde) incorporated into zein coating on inactivation of Listeria monocytogenes on a ready-to-eat meat model, frankfurters (hot dogs). Hot dogs were inoculated with 7 log CFU/sample and dipped into prepared ...


Characterization Of Bioactive Phytochemicals From The Stem Bark Of African Mahogany Khaya Senegalensis (Meliaceae), Huaping Zhang Dec 2008

Characterization Of Bioactive Phytochemicals From The Stem Bark Of African Mahogany Khaya Senegalensis (Meliaceae), Huaping Zhang

All Dissertations

ABSTRACT
African mahogany Khaya senegalensis (Desr.) A. Juss (Meliaceae) is a large tree growing mainly in the sub-Saharan savannah forests from Senegal to Uganda. This plant is one of the most popular medicinal meliaceous plants in traditional African remedies, used as a bitter tonic, folk and popular medicine against malaria, fever, mucous diarrhea, and venereal diseases as well as an anthelmintic and a taeniacide remedy. Its extracts and chemical constituents have been the subject of extensive phytochemical and pharmacological investigations since 1960s. Anti-inflammatory, anti-bacterial, anthelmintic, and antiplasmodial activities of plant extracts have been reported. Limonoids with anti-feeding, feeding deterrent and ...


Optimization Of Biodiesel Production From Crude Cottonseed Oil And Waste Vegetable Oil:Conventional And Ultrasonic Irradiation Methods, Xiaohu Fan Dec 2008

Optimization Of Biodiesel Production From Crude Cottonseed Oil And Waste Vegetable Oil:Conventional And Ultrasonic Irradiation Methods, Xiaohu Fan

All Dissertations

Biodiesel, derived from the transesterification of vegetable oils or animal fats with simple alcohols, has attracted more and more attention recently. As a cleaner burning diesel alternative, biodiesel has many attractive features including: biodegradability, nontoxicity, renewability and low emission profiles.
Although cottonseed oil was the first commercial cooking oil in the U.S, it has progressively lost its market share to some vegetable oils that have larger production and less cost. However, regarding the active researches on biodiesel production from vegetable oils, there is a promising prospective for the cottonseed oil as a feedstock for biodiesel production, which may enhance ...


Mechanisms Of Nutrition Bar Hardening: Effect Of Hydrolyzed Whey Protein And Carbohydrate Source, Shaun P. Adams Dec 2008

Mechanisms Of Nutrition Bar Hardening: Effect Of Hydrolyzed Whey Protein And Carbohydrate Source, Shaun P. Adams

All Graduate Theses and Dissertations

The influence of increasing hydrolyzed protein content on the microstructure and hardness of high protein nutrition bars was investigated to determine the mechanism of hardening during storage. Bars with various hydrolyzed protein levels were manufactured using differing ratios of 0, 25, 50, 75, 100% (wt. /wt.) of partially hydrolyzed whey protein isolate (HWPI) to an intact (non-hydrolyzed) whey protein isolate (WPI) which made up approximately 38% of the total bar composition. High fructose corn syrup (HFCS) (42%) and vegetable shortening (20%) constituted the rest of the ingredients. Accelerated aging was performed by storing bars at 32 ºC for 36 d ...


Mathematical Models Of Zea Mays: Grain Yield And Aboveground Biomass Applied To Ear Flex And Within Row Spacing Variability, Todd Curtis Ballard Dec 2008

Mathematical Models Of Zea Mays: Grain Yield And Aboveground Biomass Applied To Ear Flex And Within Row Spacing Variability, Todd Curtis Ballard

Masters Theses & Specialist Projects

Field studies were conducted during the summers of 2007 and 2008 at the Agricultural Research and Education Complex, Western Kentucky University, Warren County, KY and commercial production fields in Caldwell County, KY, Warrick County IN, and Vanderburgh County, IN. The goals of these studies were to further validate the Duncan grain yield model, the Russell aboveground biomass model, and to study the effect of inconsistent spacing within rows on Zea mays L. yield. Plant spacing other than uniform decreases grain yield and profitability. The population experiments conducted at the Warren County location were a randomized complete block design with three ...


Synthesis And Characterization Of Lactose-Amines With Respect To Oil-In-Water Emulsion Stability, Nidhi Garg Dec 2008

Synthesis And Characterization Of Lactose-Amines With Respect To Oil-In-Water Emulsion Stability, Nidhi Garg

All Graduate Theses and Dissertations

Fatty amines (hexadecyl-amine) can be esterified to lactose via Schiff-base formation at temperatures of 60° C. Extending the time of the reaction results in a darker colored product due to the Maillard reaction. Due to the amphiphilic properties of the lactose-amines, the emulsion stabilization characteristics were investigated.

In this study, synthesis of lactose-amines was done at four different heating and cooling cycles from 4 to 24 hours. Lactose-amines processed for 24 hours and 12 hours of constant heating and cooling cycles are named as 24H and 12H, respectively. Lactose-amines 4H and 8H were processed for 4 and 8 hours of ...


Effect Of Processing And Formulation Conditions On Physicochemical Characteristics Of Food Emulsions, Megan Tippetts Dec 2008

Effect Of Processing And Formulation Conditions On Physicochemical Characteristics Of Food Emulsions, Megan Tippetts

All Graduate Theses and Dissertations

The objective of this research was to systematically study the effect of processing conditions on crystallization behavior and destabilization mechanisms of oil-in-water (o/w) emulsions. The effects of oil content (20 and 40 wt %); crystallization temperature (Tc = 10, 5, 0, -5, -10 °C); homogenization conditions, such as high shear (HS), very low pressure homogenization (VLPH), and high pressure homogenization (HPH); and cooling rate (0.2 and 30 °C/min) on both thermal behavior and destabilization mechanisms were analyzed. Docosahexaenoic acid (DHA) was added to VLPH emulsions and its effect on the physicochemical and oxidative stabilities and flavor was studied. Emulsions ...


Cause And Prevention Of Liver Off-Flavor In Five Beef Chuck Muscles, Ranjeeta Wadhwani Dec 2008

Cause And Prevention Of Liver Off-Flavor In Five Beef Chuck Muscles, Ranjeeta Wadhwani

All Graduate Theses and Dissertations

Liver off-flavor is a sporadic problem that limits the consumer acceptance of several beef chuck muscles, including the infraspinatus (flat iron steak). Residual blood hemoglobin is known to contribute to liver off-flavor development. This study was conducted to evaluate factors affecting development of liver off-flavor after cooking of beef chuck (shoulder) muscles. The study was conducted in three parts. The objective of part 1 was to determine effects of muscle (infraspinatus, longissimus dorsi, serratus ventralis, supraspinatus, teres major) and processing (with or w/o carcass electrical stimulation) on residual blood hemoglobin content and total pigment content of raw muscle and ...


Melamine Contamination Of Infant Formula In China: The Causes, Food Safety Issues And Public Health Implications, Emmanuel Ohene Afoakwa Nov 2008

Melamine Contamination Of Infant Formula In China: The Causes, Food Safety Issues And Public Health Implications, Emmanuel Ohene Afoakwa

Professor Emmanuel Ohene Afoakwa

No abstract provided.


Chemical Modification Of Partially Hydrogenated Vegetable Oil To Improve Its Functional Properties To Replace Petroleum Waxes, Tong Wang, Liping Wang Nov 2008

Chemical Modification Of Partially Hydrogenated Vegetable Oil To Improve Its Functional Properties To Replace Petroleum Waxes, Tong Wang, Liping Wang

Iowa State University Patents

A novel, chemically-modified partially hydrogenated vegetable oil (PHVO) is described. The PHVO is produced by a three-step reaction process that includes epoxidation, a ringopening reaction, followed by esterification. The modified PHVO has improved kneadability and, if mixed with fully hydrogenated fat(s ), hardness comparable to umnodified PHVO.


Effect Of The Corn Breaking Method On Oil Distribution Between Stillage Phases Of Dry-Grind Corn Ethanol Production, Hui Wang, Tong Wang, Lawrence A. Johnson, Anthony L. Pometto Iii Nov 2008

Effect Of The Corn Breaking Method On Oil Distribution Between Stillage Phases Of Dry-Grind Corn Ethanol Production, Hui Wang, Tong Wang, Lawrence A. Johnson, Anthony L. Pometto Iii

Food Science and Human Nutrition Publications

The majority of fuel ethanol in the United States is produced by using the dry-grind corn ethanol process. The corn oil that is contained in the coproduct, distillers’ dried grains with solubles (DDGS), can be recovered for use as a biodiesel feedstock. Oil removal will also improve the feed quality of DDGS. The most economical way to remove oil is considered to be at the centrifugation step for separating thin stillage (liquid) from coarse solids after distilling the ethanol. The more oil there is in the liquid, the more it can be recovered by centrifugation. Therefore, we studied the effects ...


Infant-Feeding Practices Of Low-Income Vietnamese American Women, Yashmi Mistry, Marjorie Freedman, Kathleen Sweeney, Clarie Hollenbeck Nov 2008

Infant-Feeding Practices Of Low-Income Vietnamese American Women, Yashmi Mistry, Marjorie Freedman, Kathleen Sweeney, Clarie Hollenbeck

Faculty Publications

Healthy People 2010 breastfeeding goals include 50% exclusive breastfeeding at 6 months. In California, US, Santa Clara County Women, Infants, and Children (SCCWIC) data indicated Asian participants had low (5.6%) 6-month breastfeeding rates. To examine infant-feeding practices, Vietnamese breastfeeding peer counselors surveyed 133 Vietnamese SCCWIC participants (≥ 18 years old, nonpregnant, < 28 months postpartum) using a structured questionnaire regarding intentions, attitudes, and subjective norms toward breastfeeding. Results indicated that 75% initiated breastfeeding in the hospital (33% exclusively, 42% partially), and 25% exclusively formula fed. At survey time, 49% had terminated breastfeeding (average duration 4.4 months). Feeding intentions during pregnancy predicted feeding method used (P < .001). Most prenatal advice was from SCCWIC employees, friends, and doctors who supported breastfeeding. Most postnatal advice was from nurses who supported breastfeeding and bottle-feeding equally. Stronger control beliefs, peer counseling, and education were correlated with breastfeeding (P < .001). Improved postdelivery hospital advice may further increase breastfeeding initiation and duration.


Local Chef Inspires Healthy And Economical Cooking -- In A Flash, Madeleine K. Charney Nov 2008

Local Chef Inspires Healthy And Economical Cooking -- In A Flash, Madeleine K. Charney

University Librarians Publication Series

Local chef, Leslie Cerier, offers thought-provoking workshops about eating whole foods.


Understanding Consumer Preference For Functional Barley Tortillas Through Sensory, Demographic, And Behavioral Data, Ayako Toma, Maria Botero Omary, Kurt A. Rosentrater, Elizabeth A. Arndt, Naruemon Prasopsunwattana, Sutida Chongcham, Rolando A. Flores Nov 2008

Understanding Consumer Preference For Functional Barley Tortillas Through Sensory, Demographic, And Behavioral Data, Ayako Toma, Maria Botero Omary, Kurt A. Rosentrater, Elizabeth A. Arndt, Naruemon Prasopsunwattana, Sutida Chongcham, Rolando A. Flores

Kurt A. Rosentrater

Consumption of whole grains as part of a health-promoting diet is encouraged among Americans due to beneficial nutrients and phytochemicals. The main objectives of this study were to investigate the effect of whole barley flour particle size on consumer acceptance of whole grain and soluble fiber-enriched tortillas; to examine the effect of demographic and behavioral data on consumer acceptance; and to establish relationships between consumer acceptance and survey results to identify market opportunities among particular groups of consumers. Four experimental tortillas and two representative commercial brands were tested by 95 untrained panelists using a 9-point hedonic scale for appearance, color ...


Local Chef Inspires Healthy And Economical Cooking -- In A Flash, Madeleine K. Charney Nov 2008

Local Chef Inspires Healthy And Economical Cooking -- In A Flash, Madeleine K. Charney

Madeleine K. Charney

Local chef, Leslie Cerier, offers thought-provoking workshops about eating whole foods.


Commercial Evaluation Of A Continuous Micronutrient Fortification Process For Nixtamal Tortillas, Michael L. Dunn, Sergio O. Serna-Saldivar, Diana Sanchez-Hernandez, Robert W. Griffin Nov 2008

Commercial Evaluation Of A Continuous Micronutrient Fortification Process For Nixtamal Tortillas, Michael L. Dunn, Sergio O. Serna-Saldivar, Diana Sanchez-Hernandez, Robert W. Griffin

Faculty Publications

The corn tortilla plays an integral role in the Mexican diet and is an ideal vehicle for micronutrient fortification. Approximately 60% of corn tortillas in Mexico are produced from nixtamal, with the remainder prepared from masa flour. A process for continuous fortification of nixtamal tortillas was evaluated in two commercial mills in Mexico. A commercial powder dosifier was used to add micronutrient premix containing iron, zinc, folic acid, niacin, riboflavin, and thiamin to nixtamal (1 g/kg) as it was milled. After training and preliminary sampling, mills produced fortified tortillas unassisted for four weeks. Masa flow rates over a four-day ...


Infant-Feeding Practices Of Low-Income Vietnamese American Women, Yashmi Mistry, Marjorie R. Freedman, Kathleen Sweeney, Clarie Hollenbeck Oct 2008

Infant-Feeding Practices Of Low-Income Vietnamese American Women, Yashmi Mistry, Marjorie R. Freedman, Kathleen Sweeney, Clarie Hollenbeck

Marjorie R. Freedman

Healthy People 2010 breastfeeding goals include 50% exclusive breastfeeding at 6 months. In California, US, Santa Clara County Women, Infants, and Children (SCCWIC) data indicated Asian participants had low (5.6%) 6-month breastfeeding rates. To examine infant-feeding practices, Vietnamese breastfeeding peer counselors surveyed 133 Vietnamese SCCWIC participants (≥ 18 years old, nonpregnant, < 28 months postpartum) using a structured questionnaire regarding intentions, attitudes, and subjective norms toward breastfeeding. Results indicated that 75% initiated breastfeeding in the hospital (33% exclusively, 42% partially), and 25% exclusively formula fed. At survey time, 49% had terminated breastfeeding (average duration 4.4 months). Feeding intentions during pregnancy ...


The Food Processing Center News, Fall 2008 Oct 2008

The Food Processing Center News, Fall 2008

Food Science & Technology Department Records--Public Access

CONTENTS:
FDST Students Star at IFT
Membrane Filtration Opportunities for the Food Industry
Food Safety Conference a Shared Success
A Message From Dr. Flores
Dr. Hanna “Most Cited” Author of 10 Years
Alumnus Bob Bidrowski
MS Graduate Carolina Leguizamon
Graduates of the B.S. Program
Graduates of the M.S. Program
Graduates of the Ph.D. Program
UNL AWARDS
Undergraduate Interns in Home Country
Accomplishments
Graduate Student Spotlight: Tim Anderson
New M.S. Students
New Ph.D. Students
From Culture-based Methods to DNA Sequencing of Entire Ecostyems: A Major Shift in the Paradigm of Diagnostic Microbiology
Faculty/Staff
Professor of ...


Disseminated Leishmania Infantum Infection In Two Sibling Foxhounds Due To Possible Vertical Transmission, Katherine N. Gibson-Corley, Jesse M. Hostetter, Shannon J. Hostetter, Kathleen Mullin, Amanda Ramer-Tait, Paola M. Boggiatto, Christine A. Petersen Oct 2008

Disseminated Leishmania Infantum Infection In Two Sibling Foxhounds Due To Possible Vertical Transmission, Katherine N. Gibson-Corley, Jesse M. Hostetter, Shannon J. Hostetter, Kathleen Mullin, Amanda Ramer-Tait, Paola M. Boggiatto, Christine A. Petersen

Faculty Publications in Food Science and Technology

Two sibling foxhounds born to a Leishmania seropositive bitch were presented after testing seropositive for Leishmania. Leishmania infantum infection was detected via histopathology, culture, and quantitative polymerase chain reaction (q-PCR). This is the first report of natural infection with Leishmania infantum with the possibility for vertical transmission in North America.

Infection disséminée à Leishmania infantum chez deux chiots Fox hound d’une même portée reliée possiblement à une transmission verticale. Deux chiots Fox hound d’une même portée nés d’une mère séropositive à Leishmania ont été présentés après un contrôle sérologique positif. Une infection à Leishmania infantum a été ...


Food Allergy Alert—What Restaurant Managers Need To Know To Train Staff, Jill Brierly, Catherine Strohbehn, Sam Beattie, Diane Nelson Oct 2008

Food Allergy Alert—What Restaurant Managers Need To Know To Train Staff, Jill Brierly, Catherine Strohbehn, Sam Beattie, Diane Nelson

Human Sciences Extension and Outreach Publications

Allergic reactions to menu items are a growing concern for restaurant managers. This guide will help managers develop in-house training sessions for all staff members.


Got Controversy - Milk Does, Margaret Sova Mccabe Oct 2008

Got Controversy - Milk Does, Margaret Sova Mccabe

Law Faculty Scholarship

This article analyzes ongoing controversy over how to best label rBST-free milk. Recombinant bovine somatotropin is a genetically engineered drug administered by some farmers to their dairy herds to increase milk production. FDA first approved its use in 1994, despite great controversy. The FDA also issued labeling guidelines that allowed voluntary disclosure of rBST-free milk, so long as it carried the disclaimer that no difference could be detected between milk produced with rBST and rBST-free. The controversy continues today as consumers express a preference for rBST-free milk and many rBST-free producers label their milk this way. "Conventional" milk (with rBST ...


Effects Of Filtration Temperature And Heat Treatment On Composition And Rheological Properties Of Whole Milk Ultrafiltration Retentates, John W. Montella Oct 2008

Effects Of Filtration Temperature And Heat Treatment On Composition And Rheological Properties Of Whole Milk Ultrafiltration Retentates, John W. Montella

Master's Theses and Project Reports

ABSTRACT

Effects of Filtration Temperature and Heat Treatment on Composition and Rheological Properties of Whole Milk Ultrafiltration Retentates

John William Montella

For the first part of my thesis, the effects of filtration temperature and heat treatment on the compositional properties of whole milk Ultrafiltration retentate (UF) were studied. Ultrafiltration is primarily run at temperatures in the range of 50-55°C but more and more plants are starting to filter at refrigeration temperatures. In the ultrafiltration of milk, filtration temperature can affect the composition of the retentate by affecting the chemistry of milk components. The application of a pasteurization step can ...


Boiling Water Canning Lemon Or Lime Curd, Brian Nummer Oct 2008

Boiling Water Canning Lemon Or Lime Curd, Brian Nummer

All Current Publications

This publication includes recipe and instructions on canning lemon and lime curd using boiling water method.


Synthesis And Antimicrobial Evaluation Of Carbohydrate And Polyhydroxylated Non-Carbohydrate Fatty Acid Ester And Ether Derivatives, Aoife Smith, Patricia Nobmann, Gary Henehan, Paula Bourke, Julie Dunne Oct 2008

Synthesis And Antimicrobial Evaluation Of Carbohydrate And Polyhydroxylated Non-Carbohydrate Fatty Acid Ester And Ether Derivatives, Aoife Smith, Patricia Nobmann, Gary Henehan, Paula Bourke, Julie Dunne

Articles

A series of fatty acid ester and ether derivatives have been chemically synthesised based on carbohydrate and non-carbohydrate polyhydroxylated scaffolds. The synthesised compounds, along with their corresponding fatty acid monoglyceride antimicrobials, were evaluated for antimicrobial activity against Staphylococcus aureus and Escherichia coli. Of the derivatives synthesised several of the carbohydrate based compounds have antimicrobial efficacy comparable with commercially available antimicrobials. The results suggest that the nature of the carbohydrate core plays a role in the efficacy of carbohydrate fatty acid derivatives as antimicrobials.


Modelling Tempering Behaviour Of Dark Chocolates From Varying Particle Size Distribution And Fat Content Using Response Surface Methodology, Emmanuel Ohene Afoakwa, Alistair Paterson, Mark Fowler, Joselio Vieira Sep 2008

Modelling Tempering Behaviour Of Dark Chocolates From Varying Particle Size Distribution And Fat Content Using Response Surface Methodology, Emmanuel Ohene Afoakwa, Alistair Paterson, Mark Fowler, Joselio Vieira

Professor Emmanuel Ohene Afoakwa

Central Composite Rotatable Design (CCRD) for K=2 was used to study the combined effects of multi-stage heat exchangers for Stages 1 (14–30 °C) and 2 (12–28 °C) coolant temperatures at constant Stage 3 coolant and holding temperatures during tempering of dark chocolates using laboratory-scale mini-temperer. Quantitative data on chocolate temper index (slope) were obtained for products with varying particle size distribution (PSD) (D90 of 18, 25, 35 and 50 μm) and fat (30% and 35%) content. Regression models generated using stepwise regression analyses were used to plot response surface curves, to study the tempering behaviour of products ...


Impact Of Dry Solids And Bile Acid Concentrations On Bile Acid Binding Capacity Of Extruded Oat Cereals, Ni Yao, Pamela J. White, Jean-Luc Jannink, Salid Alavi Sep 2008

Impact Of Dry Solids And Bile Acid Concentrations On Bile Acid Binding Capacity Of Extruded Oat Cereals, Ni Yao, Pamela J. White, Jean-Luc Jannink, Salid Alavi

Food Science and Human Nutrition Publications

Extruded breakfast cereals (EBC), processed from two oat lines, N979-5-2-4 (N979) and “Jim”, with β-glucan concentrations of 8.7 and 4.9%, respectively, were used to determine the impact of dry solids (DS) and bile acid (BA) concentrations on in vitro BA binding efficiency. A full fractional factorial design with levels for BA concentrations of 0.20, 0.47, 0.95, 2.37, and 4.73 μmol/g of total EBC slurry and for DS in the slurries of 0.8, 2, 3, and 4% (w/w) was selected. The absolute amount of BA bound (μmol) was measured for each ...


Next Generation Dna Sequencing And The Birth Of A Golden Era For Microbial Ecology Sep 2008

Next Generation Dna Sequencing And The Birth Of A Golden Era For Microbial Ecology

Core for Applied Genomics and Ecology (CAGE)

In 2005, a breakthrough technology was Announced that provided a quantum leap In DNA sequencing capacity.

This technology differed from the Sanger method In many ways:
1. Eliminates cloning—completely circumvents cloning bias
2. Uses bead technology—DNA templates are attached to sepharose beads
3. Relies on pyrosequencing chemistry—synthesis is sequential not partial
4. Parallelizes the sequencing process—hundreds of thousands of beads can be sequenced in parallel

The bottom line: it makes production-scale sequencing available to the common man.