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Full-Text Articles in Life Sciences

Effect Of Heat Treatment, Irradiation And Storage Conditions On The Survival, Growth And Virulence Of Heat-Shocked Yersinia Enterocolitica In Ground Pork , Kalpana Shenoy Jan 1995

Effect Of Heat Treatment, Irradiation And Storage Conditions On The Survival, Growth And Virulence Of Heat-Shocked Yersinia Enterocolitica In Ground Pork , Kalpana Shenoy

Retrospective Theses and Dissertations

The heat shock response and thermotolerance of Yersinia enterocolitica in BHI broth and ground pork was studied. Further, the survival and growth of the heat-shocked cells following heat treatment at 55° C and stored at 4 or 25° C under air, vacuum, or modified atmosphere was also examined. Lastly, the irradiation sensitivity of the heat-shocked cells when suspended in pork and packaged either under air or vacuum was determined. As part of the research, virulence of the survivors was examined following heat treatment or irradiation;Exposure of Y. enterocolitica to 45° C for 60 mins. in BHI broth resulted in ...


Delayed-Incubation Method For Microbiological Analysis Of Environmental Specimens And Samples, James S. Dickson, M. H. Brodsky, C. A. Davidson, M. J. Pettis, T. L. Tieso Jan 1995

Delayed-Incubation Method For Microbiological Analysis Of Environmental Specimens And Samples, James S. Dickson, M. H. Brodsky, C. A. Davidson, M. J. Pettis, T. L. Tieso

James S. Dickson

Five laboratories compared the quantitative recovery of heterotrophic bacteria, yeasts and molds, coliforms, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus from a variety of naturally contaminated food and water samples, using traditional incubation procedures and a delayed-incubation method. Refrigeration of inoculated media for up to 3 days prior to incubation was shown to be a viable option for many quantitative analyses, but needs to be validated for each application. Some inoculated media withstood refrigeration for up to 7 days prior to incubation without any detrimental effect on the recovery of target cells, while the incubation of other media for similar ...