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Food Science

1995

Animal science

Articles 1 - 3 of 3

Full-Text Articles in Life Sciences

Myofibrillar Protein Degradation Patterns And Structural Changes In Skeletal Muscle From Electrically Stimulated Bos Taurus And Bos Indicus Crossbred Cattle , Chiung-Ying Ho Jan 1995

Myofibrillar Protein Degradation Patterns And Structural Changes In Skeletal Muscle From Electrically Stimulated Bos Taurus And Bos Indicus Crossbred Cattle , Chiung-Ying Ho

Retrospective Theses and Dissertations

Degradation of titin, nebulin, desmin and troponin-T, structural changes in nonstimulated (NS) and electrically stimulated (ES) bovine skeletal muscle and comparison of these changes in Angus x Jersey (AxJ) cattle (Bos taurus cross) with the changes in Brahman x Simmental (BxS) cattle (Bos indicus cross) were determined. Myofibrils for SDS-PAGE and Western blots and intact muscle samples for transmission electron microscopy were prepared at 0, 1, 3, 7, 14 and 28 days postmortem (PM). In SDS-PAGE, ES slightly accelerated the degradation of intact titin (T1 band), nebulin, desmin and troponin-T in AxJ samples. In Western blots of AxJ samples, ES ...


The Relationship Between The Calpain Enzyme System And The Postmortem Degradation Of Selected Myofibrillar Proteins , Elisabeth Jane Huff Lonergan Jan 1995

The Relationship Between The Calpain Enzyme System And The Postmortem Degradation Of Selected Myofibrillar Proteins , Elisabeth Jane Huff Lonergan

Retrospective Theses and Dissertations

The protease [mu]-calpain degrades the myofibrillar proteins titin, nebulin, filamin, desmin and troponin-T. Titin and nebulin have been reported to be affected by sample preparation. This study showed that there was little difference in the rate of degradation of the intact forms due to sample preparation (whole muscle preparations vs. purified myofibrils). The postmortem degradation of titin, nebulin, filamin, desmin, and troponin-T was monitored using SDS-PAGE and Western blotting techniques at 0, 1, 3, 7, 14, 28, and 56 days postmortem. Postmortem aged samples with lower shear force values exhibited faster degradation of the five myofibrillar proteins. Similar degradation ...


Use Of Eubacterium Coprostanoligenes To Decrease Plasma Cholesterol Concentration In Hypercholesterolemic Rabbits And The Cholesterol Content Of Fermented Meats , Uford Augustus Madden Jan 1995

Use Of Eubacterium Coprostanoligenes To Decrease Plasma Cholesterol Concentration In Hypercholesterolemic Rabbits And The Cholesterol Content Of Fermented Meats , Uford Augustus Madden

Retrospective Theses and Dissertations

The effects of Eubacterium coprostanoligenes, a cholesterol reducing bacteria, on plasma cholesterol homeostasis in hypercholesterolemic rabbits and on the cholesterol content of pork and mutton sausages were investigated. Daily oral doses of the bacteria were administered to hypercholesterolemic rabbits, and meat samples were inoculated with different amounts of bacteria and incubated for 12-15 hours at 37° C. These studies addressed the problem of human hypercholesterolemia by investigating the possibility of using E. coprostanoligenes as a model for the treatment of human hypercholesterolemia and to develop technology to decrease the cholesterol content of foods;Eighteen New Zealand White male adult rabbits ...