Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

PDF

Food Science

1995

Faculty Publications in Food Science and Technology

Articles 1 - 2 of 2

Full-Text Articles in Life Sciences

Cloning And Characterization Of The Galactokinase Gene From Streptococcus Thermophilus, Azlin Mustapha, Robert W. Hutkins, Gerald W. Hirnstein Jan 1995

Cloning And Characterization Of The Galactokinase Gene From Streptococcus Thermophilus, Azlin Mustapha, Robert W. Hutkins, Gerald W. Hirnstein

Faculty Publications in Food Science and Technology

The objective of this research was to clone and characterize the galactokinase gene (gal0 from Streptococcus therrnophilus F4 10. Partially digested genomic DNA was cloned into pBR322 and transformed into galK Escherichia coli, and a galactose-fermenting transformant was isolated. Restriction analysis revealed that the transformant resulted from a Sau3A-HindIIl 4.0-kb fragment. Galactokinase activity in the recombinant was 10 times that of the parent strain. Analysis of the DNA squence showed the presence of a 1.3-kb open reading frame that had high homology with the galK gene from other organisms. A putative ribosome-binding site, start and stop codons, and ...


Global Regulation Of A Σ54-Dependent Flagellar Gene Family In Caulobacter Crescentus By The Transcriptional Activator Flbd, Jianguo Wu, Andrew K. Benson, Austin Newton Jan 1995

Global Regulation Of A Σ54-Dependent Flagellar Gene Family In Caulobacter Crescentus By The Transcriptional Activator Flbd, Jianguo Wu, Andrew K. Benson, Austin Newton

Faculty Publications in Food Science and Technology

Biosynthesis of the Caulobacter crescentus polar flagellum requires the expression of a large number of flagellar (fla) genes that are organized in a regulatory hierarchy of four classes (I to IV). The timing of fla gene expression in the cell cycle is determined by specialized forms of RNA polymerase and the appearance and/or activation of regulatory proteins. Here we report an investigation of the role of the C. crescentus transcriptional regulatory protein FlbD in the activation of σ54-dependent class III and class IV fla genes of the hierarchy by reconstituting transcription from these promoters in vitro. Our ...