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Food Science

1995

Entomology Publications

Articles 1 - 1 of 1

Full-Text Articles in Life Sciences

Evaluation Of A Liquid Chromatographic Method For The Determination Of Fumonisins In Corn, Poultry Feed, And Fusarium Culture Material., Larry G. Rice, P. Frank Ross, Jan Dejong, Ronald D. Plattner, Joel R. Coats Jul 1995

Evaluation Of A Liquid Chromatographic Method For The Determination Of Fumonisins In Corn, Poultry Feed, And Fusarium Culture Material., Larry G. Rice, P. Frank Ross, Jan Dejong, Ronald D. Plattner, Joel R. Coats

Entomology Publications

The performance of a liquid chromatographic method for determining fumonisins in corn, animal feeds, and culture material was evaluated. Efficiencies of extractions with the following solvent systems were determined: acetonitrile-water (50 + 50, v/v), methanol-water (75 + 25, v/v), and 100% water. The acetonitrile solvent gave both higher extraction efficiencies and faster extraction times than the other 2 solvents. Extraction was followed by C18 solid-phase extraction column cleanup. Fumonisin B1 (FB1), fumonisin B2 (FB2), and fumonisin B3 (FB3) were measured by precolumn derivatization with o-phthalaldehyde followed by isocratic separation on a C18 reversed-phase column with a mobile phase of 50 ...