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Food Science

Food Science and Human Nutrition Publications

Echinacea angustifolia

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Full-Text Articles in Life Sciences

Enrichment Of Echinacea Angustifolia With Bauer Alkylamide 11 And Bauer Ketone 23 Increased Anti-Inflammatory Potential Through Interference With Cox-2 Enzyme Activity, Carlie A. Lalone, Nan Huang, Ludmila Rizshsky, Man-Yu Yum, Navrozedeep Singh, Cathy Hauck, Basil J. Nikolau, Eve S. Wurtele, Marian L. Kohut, Patricia A. Murphy, Diane F. Birt Jan 2010

Enrichment Of Echinacea Angustifolia With Bauer Alkylamide 11 And Bauer Ketone 23 Increased Anti-Inflammatory Potential Through Interference With Cox-2 Enzyme Activity, Carlie A. Lalone, Nan Huang, Ludmila Rizshsky, Man-Yu Yum, Navrozedeep Singh, Cathy Hauck, Basil J. Nikolau, Eve S. Wurtele, Marian L. Kohut, Patricia A. Murphy, Diane F. Birt

Food Science and Human Nutrition Publications

Bauer alkylamide 11 and Bauer ketone 23 were previously found to be partially responsible forEchinacea angustifolia anti-inflammatory properties. This study further tested their importance using the inhibition of prostaglandin E2 (PGE2) and nitric oxide (NO) production by RAW264.7 mouse macrophages in the absence and presence of lipopolysaccharide (LPS) and E. angustifolia extracts, phytochemical enriched fractions, or pure synthesized standards. Molecular targets were probed using microarray, qRT-PCR, Western blot, and enzyme assays. Fractions with these phytochemicals were more potent inhibitors of LPS-induced PGE2 production than E. angustifolia extracts. Microarray did not detect changes in transcripts with ...


Endogenous Levels Of Echinacea Alkylamides And Ketones Are Important Contributors To The Inhibition Of Prostaglandin E2 And Nitric Oxide Production In Cultured Macrophages, Carlie A. Lalone, Ludmila Rizshsky, Kimberly D.P. Hammer, Lankun Wu, Avery K.S. Solco, Man-Yu Yum, Basil J. Nikolau, Eve S. Wurtele, Patricia A. Murphy, Meehye Kim, Diane F. Birt Jan 2009

Endogenous Levels Of Echinacea Alkylamides And Ketones Are Important Contributors To The Inhibition Of Prostaglandin E2 And Nitric Oxide Production In Cultured Macrophages, Carlie A. Lalone, Ludmila Rizshsky, Kimberly D.P. Hammer, Lankun Wu, Avery K.S. Solco, Man-Yu Yum, Basil J. Nikolau, Eve S. Wurtele, Patricia A. Murphy, Meehye Kim, Diane F. Birt

Food Science and Human Nutrition Publications

Because of the popularity of Echinacea as a dietary supplement, researchers have been actively investigating which Echinacea constituent or groups of constituents are necessary for immunemodulating bioactivities. Our prior studies indicate that alkylamides may play an important role in the inhibition of prostaglandin E2 (PGE2) production. High-performance liquid chromatography fractionation, employed to elucidate interacting anti-inflammatory constituents from ethanol extracts of Echinacea purpurea, Echinacea angustifolia, Echinacea pallida, and Echinacea tennesseensis, identified fractions containing alkylamides and ketones as key anti-inflammatory contributors using lipopolysaccharideinduced PGE2 production in RAW264.7 mouse macrophage cells. Nitric oxide (NO) production and parallel cytotoxicity screens were also employed ...


Echinacea Species And Alkamides Inhibit Prostaglandin E2 Production In Raw264.7 Mouse Macrophage Cells, Carlie A. Lalone, Kimberly D.P. Hammer, Lankun Wu, Jaehood Bae, Norma Leyva, Yi Liu, Avery K.S. Solco, George A. Kraus, Patricia A. Murphy, Eve S. Wurtele, Ok-Kyung Kim, Kwon Ii Seo, Mark P. Widrlechner, Diane F. Birt Jan 2007

Echinacea Species And Alkamides Inhibit Prostaglandin E2 Production In Raw264.7 Mouse Macrophage Cells, Carlie A. Lalone, Kimberly D.P. Hammer, Lankun Wu, Jaehood Bae, Norma Leyva, Yi Liu, Avery K.S. Solco, George A. Kraus, Patricia A. Murphy, Eve S. Wurtele, Ok-Kyung Kim, Kwon Ii Seo, Mark P. Widrlechner, Diane F. Birt

Food Science and Human Nutrition Publications

Inhibition of prostaglandin E2 (PGE2) production in lipopolysaccharide-stimulated RAW264.7 mouse macrophage cells was assessed with an enzyme immunoassay following treatments with Echinacea extracts or synthesized alkamides. Results indicated that ethanol extracts diluted in media to a concentration of 15 μg/mL from E. angustifolia, E. pallida, E. simulata, and E. sanguinea significantly inhibited PGE2 production. In further studies, PGE2 production was significantly reduced by all synthesized alkamides assayed at 50 μM, by Bauer alkamides 8, 12A analogue, and 14, Chen alkamide 2, and Chen alkamide 2 analogue at 25 μM and by Bauer alkamide 14 ...