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Food Science

Food Science and Human Nutrition Publications

Department of Genetics

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Full-Text Articles in Life Sciences

Genetic Modification Of Low Phytic Acid 1-1 Maize To Enhance Iron Content And Bioavailability, Maneesha R. Aluru, Steve R. Rodermel, Manju B. Reddy Nov 2011

Genetic Modification Of Low Phytic Acid 1-1 Maize To Enhance Iron Content And Bioavailability, Maneesha R. Aluru, Steve R. Rodermel, Manju B. Reddy

Food Science and Human Nutrition Publications

High phytate content in staple food crops is a major barrier to successful iron biofortification. We have exploited the low phytic acid 1-1 (lpa1-1) mutant of maize to generate transgenic plants with up-to 70 μg/g seed iron through the endosperm-specific overexpression of soybean ferritin, resulting in more than 2-fold improvement in iron bioavailability. The levels of bioavailable seed iron achieved in this study greatly exceed any achieved thus far and closely approach values estimated to have a nutritional impact on target populations. Gene expression studies reveal a large induction of the YS1 transporter in leaves and severe repression of ...


Quantification Of Ferritin From Staple Food Crops, Rebbecca J. Lukac, Maneesha R. Aluru, Manju B. Reddy Mar 2009

Quantification Of Ferritin From Staple Food Crops, Rebbecca J. Lukac, Maneesha R. Aluru, Manju B. Reddy

Food Science and Human Nutrition Publications

Ferritin-iron has been shown to be as bioavailable as ferrous sulfate in humans. Thus, biofortification to breed crops with high ferritin content is a promising strategy to alleviate the global iron deficiency problem. Although ferritin is present in all food crops, its concentration varies between species and varieties. Therefore, a successful ferritin biofortification strategy requires a method to rapidly measure ferritin concentrations in food crops. The objective of this study was to develop a simple and reliable ELISA using an anti-ferritin polyclonal antibody to detect ferritin in various crops. Crude seed extracts were found to have 10.2 +/- 1.0 ...


Oxalate And Phytate Concentrations In Seeds Of Soybean Cultivars, Harry T. Horner, Teresa Cervantes-Martinez, Rosanne Healy, Manju B. Reddy, Betsy L. Deardorff, Theodore B. Bailey, Ismail Al-Wahsh, Linda K. Massey, Reid G. Palmer Sep 2005

Oxalate And Phytate Concentrations In Seeds Of Soybean Cultivars, Harry T. Horner, Teresa Cervantes-Martinez, Rosanne Healy, Manju B. Reddy, Betsy L. Deardorff, Theodore B. Bailey, Ismail Al-Wahsh, Linda K. Massey, Reid G. Palmer

Food Science and Human Nutrition Publications

This study analyzed soybean seeds from 116 cultivars for total, insoluble, and soluble oxalate (Ox), phytate (InsP6), calcium (Ca), and magnesium (Mg) because of their potential beneficial or harmful effects on human nutrition. These cultivars were divided into four groups (A-D) on the basis of the year and geographic location where they were grown. Oxalate concentration ranged from about 82 to 285 mg/100 g of dry seed. The InsP6 concentration ranged from 0.22 to 2.22 g/100 g of dry seed. There was no correlation between Ox and InsP6 within or among the four groups of cultivars ...