Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 18 of 18

Full-Text Articles in Life Sciences

Phage Based Electrochemical Detection Of Escherichia Coli In Drinking Water Using Affinity Reporter Probes, Danhui Wang, Troy Hinkley, Juhong Chen, Joey Talbert, Sam R. Nugen Dec 2018

Phage Based Electrochemical Detection Of Escherichia Coli In Drinking Water Using Affinity Reporter Probes, Danhui Wang, Troy Hinkley, Juhong Chen, Joey Talbert, Sam R. Nugen

Food Science and Human Nutrition Publications

The monitoring of drinking water for indicators of fecal contamination is crucial for ensuring a safe supply. In this study, a novel electrochemical method was developed for the rapid and sensitive detection of Escherichia coli (E. coli) in drinking water. This strategy is based on the use of engineered bacteriophages (phages) to separate and concentrate target E. coli when conjugated with magnetic beads, and to facilitate the detection by expressing gold binding peptides fused alkaline phosphatase (GBPs-ALP). The fusion protein GBPs-ALP has both the enzymatic activity and the ability to directly bind onto a gold surface. This binding-peptide mediated immobilization ...


Development Of A Food Safety Training For A Prison Farm: Challenges And Solutions, Angela M. Shaw, Kara Helterbran Dec 2018

Development Of A Food Safety Training For A Prison Farm: Challenges And Solutions, Angela M. Shaw, Kara Helterbran

Food Science and Human Nutrition Publications

Workers on prison farms in the United States commonly grow produce and row crops and raise livestock. Teaching good agricultural practices to prisoners and prison staff increases the safety of the food produced and reduces the likelihood of an illness outbreak. We developed a food safety curriculum for use on prison farms in Iowa. In developing the curriculum, we encountered many challenges that resulted in modifications in the content and delivery method. We present the content of the curriculum and provide educators with a better understanding of challenges and potential solutions related to working with prison farms.


Determining What Growers Need To Comply With The Food Safety Modernization Act Produce Safety Rule, Catherine H. Strohbehn, Arlene E. Enderton, Angela M. Shaw, Bridget J. Perry, Jacques Overdiep, Linda Naeve Dec 2018

Determining What Growers Need To Comply With The Food Safety Modernization Act Produce Safety Rule, Catherine H. Strohbehn, Arlene E. Enderton, Angela M. Shaw, Bridget J. Perry, Jacques Overdiep, Linda Naeve

Food Science and Human Nutrition Publications

Extension educators have been enlisted to assist farmers in meeting requirements of the Food Safety Modernization Act Produce Safety Rule (PSR). Although food safety is a familiar topic for Extension educators, helping farmers learn how to prepare for PSR regulations is new. In this article, we describe a needs assessment conducted in the north central United States according to a modified Delphi approach. Results revealed unique characteristics of farmers in the region, least understood components of the PSR, preferences regarding educational tools, and the need for materials for varied audiences. Our process can be adapted for the purpose of determining ...


Capture, Concentration And Detection Of Salmonella In Foods Using Magnetic Ionic Liquids And Recombinase Polymerase Amplification, Stephanie A. Hice, Kevin D. Clark, Jared L. Anderson, Byron F. Brehm-Stecher Nov 2018

Capture, Concentration And Detection Of Salmonella In Foods Using Magnetic Ionic Liquids And Recombinase Polymerase Amplification, Stephanie A. Hice, Kevin D. Clark, Jared L. Anderson, Byron F. Brehm-Stecher

Food Science and Human Nutrition Publications

We have previously investigated the extraction and concentration of bacteria from model systems using magnetic ionic liquid (MIL) solvents, while retaining their viability. Here, we combine MIL-based sample preparation with isothermal amplification and detection of Salmonella-specific DNA using Recombinase Polymerase Amplification (RPA). After initial developmental work with Serratia marcescens in water, Salmonella Typhimurium ATCC 14028 was inoculated in water, 2% milk, almond milk or liquid egg samples and extracted using one of two MILs, including: trihexyl(tetradecyl)phosphonium cobalt(II) hexafluoroacetylacetonate ([P66614+][Co(hfacac)3–]) and trihexyl(tetradecyl)phosphonium nickel(II) hexafluoroacetylacetonate ([P66614+][Ni(hfacac)3–]). Viable cells were ...


Perceptions Of Heart-Healthy Behaviors Among African American Adults: A Mixed Methods Study, Cheryl Der Ananian, Donna M. Winham, Sharon V. Thompson, Megan E. Tisue Nov 2018

Perceptions Of Heart-Healthy Behaviors Among African American Adults: A Mixed Methods Study, Cheryl Der Ananian, Donna M. Winham, Sharon V. Thompson, Megan E. Tisue

Food Science and Human Nutrition Publications

African Americans have a disproportionately higher risk of chronic conditions such as cardiovascular disease (CVD), type 2 diabetes, and hypertension than other ethnic or racial groups. Data regarding CVD-related perceptions and beliefs among African Americans are limited, particularly in the Southwest US. Assessment of current views regarding health and health behaviors is needed to tailor interventions to meet the unique needs of specific populations. We sought to examine knowledge, attitudes, and perceptions of African Americans living in Arizona toward CVD and etiological factors associated with health behaviors and chronic disease development to inform state health agency program development. Transcripts from ...


A Phage-Based Assay For The Rapid, Quantitative, And Single Cfu Visualization Of E. Coli (Ecor #13) In Drinking Water, Troy C. Hinkely, S. Singh, Spencer Garing, Anne-Laure M. Le Ny, Kevin P. Nichols, Joseph E. Peters, Joey N. Talbert, Sam R. Nugen Oct 2018

A Phage-Based Assay For The Rapid, Quantitative, And Single Cfu Visualization Of E. Coli (Ecor #13) In Drinking Water, Troy C. Hinkely, S. Singh, Spencer Garing, Anne-Laure M. Le Ny, Kevin P. Nichols, Joseph E. Peters, Joey N. Talbert, Sam R. Nugen

Food Science and Human Nutrition Publications

Drinking water standards in the United States mandate a zero tolerance of generic E. coli in 100mL of water. The presence of E. coli in drinking water indicates that favorable environmental conditions exist that could have resulted in pathogen contamination. Therefore, the rapid and specifc enumeration of E. coli in contaminated drinking water is critical to mitigate signifcant risks to public health. To meet this challenge, we developed a bacteriophage-based membrane fltration assay that employs novel fusion reporter enzymes to fully quantify E. coli in less than half the time required for traditional enrichment assays. A luciferase and an alkaline ...


Socio-Ecological Barriers To Dry Grain Pulse Consumption Among Low-Income Women: A Mixed Methods Approach, Shelly M. Palmer, Donna M. Winham, Ann M. Oberhauser, Ruth E. Litchfield Aug 2018

Socio-Ecological Barriers To Dry Grain Pulse Consumption Among Low-Income Women: A Mixed Methods Approach, Shelly M. Palmer, Donna M. Winham, Ann M. Oberhauser, Ruth E. Litchfield

Food Science and Human Nutrition Publications

The purpose of this study was to determine the socio-ecological influences on dry grain pulse consumption (beans, peas, lentils, chickpeas) among low-socioeconomic women in Iowa. Seven focus groups were conducted, with 36 women who qualified for income-based federal assistance. Data were collected from October 2017 to January 2018. Participants completed a survey that gathered individual demographics, assessed perceptions of dry grain pulses, and level of food security. Fifty-eight percent of the women were non-Hispanic white, and 39% were African American, all with an average age of 34.7 years. Thirty-three percent of the women consumed pulses less than once per ...


Extraction Of Phospholipids From A Dairy By-Product (Whey Protein Phospholipid Concentrate) Using Ethanol, Nathan R. Price, Tao Fei, Stephanie Clark, Tong Wang Aug 2018

Extraction Of Phospholipids From A Dairy By-Product (Whey Protein Phospholipid Concentrate) Using Ethanol, Nathan R. Price, Tao Fei, Stephanie Clark, Tong Wang

Food Science and Human Nutrition Publications

There has been a great interest in the phospholipids (PL) found in dairy products because of their health and functional properties. In this study, a technology that was originally developed for egg yolk PL extraction was applied to whey protein phospholipid concentrate (WPPC). This method successfully precipitated the proteins present in WPPC and extracted the lipids with a renewable alcoholic solvent, ethanol. The effect of ethanol concentration, extraction temperature, and extraction number on the recovery of total lipid, total PL, and individual PL class was evaluated. The optimum processing conditions for a combined 5-stage sequential extraction for producing a PL-enriched ...


Reporter Bacteriophage T7nlc Utilizes A Novel Nanoluc::Cbm Fusion For The Ultrasensitive Detection Of Escherichia Coli In Water., T.C. Hinkley, S. Garing, S. Singh, A-L. M. Le Ny, K.P. Nichols, J.E. Peters, Joey N. Talbert, S.R. Nugen Aug 2018

Reporter Bacteriophage T7nlc Utilizes A Novel Nanoluc::Cbm Fusion For The Ultrasensitive Detection Of Escherichia Coli In Water., T.C. Hinkley, S. Garing, S. Singh, A-L. M. Le Ny, K.P. Nichols, J.E. Peters, Joey N. Talbert, S.R. Nugen

Food Science and Human Nutrition Publications

Rapid detection of bacteria responsible for foodborne diseases is a growing necessity for public health. Reporter bacteriophages (phages) are robust biorecognition elements uniquely suited for the rapid and sensitive detection of bacterial species. The advantages of phages include their host specificity, ability to distinguish viable and non-viable cells, low cost, and ease of genetic engineering. Upon infection with reporter phages, target bacteria express reporter enzymes encoded within the phage genome. In this study, the T7 coliphage was genetically engineered to express the newly developed luceriferase, NanoLuc (NLuc), as an indicator of bacterial contamination. While several genetic approaches were employed to ...


Latinos Living Well—A Diabetes Education Program For Rural-Residing Latinos, Michelle N. Keane, Sarah L. Francis Aug 2018

Latinos Living Well—A Diabetes Education Program For Rural-Residing Latinos, Michelle N. Keane, Sarah L. Francis

Food Science and Human Nutrition Publications

Diabetes is a health issue for many Latinos. Extension can help provide diabetes self-management education for rural-residing Latinos. In a pilot study, we evaluated the Extension-delivered diabetes program Latinos Living Well (LLW), as provided to 76 rural-residing Latino adults in a midwestern state. LLW consists of four weekly lessons that include hands-on activities and cooking practicums. Participants completed diabetes self-management, self-efficacy, and knowledge questionnaires; significant (p ≤ .05) changes from before to after the program were detected for each outcome measure. Additionally, participants viewed LLW favorably. Results suggest that LLW is an effective, culturally relevant diabetes self-management education program for Latinos ...


Changes And Degradation Kinetics Of Some Bioactive Compounds In Dried Orthosiphon Aristatus (Java Tea) Leaves During Elevated Temperature Storage, Vorapong Klungboonkrong, Buddhi P. Lamsal, Singhanat Phoungchandang Jul 2018

Changes And Degradation Kinetics Of Some Bioactive Compounds In Dried Orthosiphon Aristatus (Java Tea) Leaves During Elevated Temperature Storage, Vorapong Klungboonkrong, Buddhi P. Lamsal, Singhanat Phoungchandang

Food Science and Human Nutrition Publications

BACKGROUND Orthosiphon aristatus is a traditional medicinal herb mostly used in South East Asia, and has many health benefits. The packaging types and storage temperatures were investigated in order to select the best condition to produce high bioactive compounds (BC) of two kinds of dried Orthosiphon aristatus leaves.

RESULTS Blanched leaves were vacuum packed in polypropylene (PP) and aluminum foil laminated with polyethylene terephthalate and polyethylene (PET/Al/PE) and dried in a freeze dryer (B_FD) or heat pump‐assisted dehumidified dryer (B_HPD60) at 60°C prior to storage at 15°, 25° and 35°C for 6 months. Leaves in ...


Session 3 Discussion: The Microstructure Of Eating, Cordelia Running, Bryony James, James Hollis, Kelly Higgins May 2018

Session 3 Discussion: The Microstructure Of Eating, Cordelia Running, Bryony James, James Hollis, Kelly Higgins

Food Science and Human Nutrition Publications

The Microstructure of Eating.


Health Behaviors Among Low-Income Hispanic And Non-Hispanic White Women, Donna M. Winham, Shelly M. Palmer, Traci L. Armstrong Florian, Mack C. Shelley May 2018

Health Behaviors Among Low-Income Hispanic And Non-Hispanic White Women, Donna M. Winham, Shelly M. Palmer, Traci L. Armstrong Florian, Mack C. Shelley

Food Science and Human Nutrition Publications

Objectives: We determined relationships between food behaviors and health-risk factors by acculturation among limited-income Hispanic and non-Hispanic white women. Methods: Women aged 18-49 years were recruited from income-based programs in metro-Phoenix, Arizona. Self-administered surveys in English or Spanish included demographics, a 10-item food behavior checklist, health-risk factors, food security, and acculturation. Differences by 4 acculturation/ ethnicity categories were assessed with chi-square and analysis of variance (ANOVA). We created a food behavior scale. Results: Eighty-two percent self-identified as Hispanic (N = 358), with 45% Hispanic-dominant, 25% bicultural, 12% English-dominant, and 18% non-Hispanic white for acculturation status. Food behavior checklist results showed that ...


Arizona Registered Dietitians Show Gaps In Knowledge Of Bean Health Benefits, Donna M. Winham, Andrea M. Hutchins, Sharon V. Thompson, Mariah K. Dougherty Jan 2018

Arizona Registered Dietitians Show Gaps In Knowledge Of Bean Health Benefits, Donna M. Winham, Andrea M. Hutchins, Sharon V. Thompson, Mariah K. Dougherty

Food Science and Human Nutrition Publications

Registered Dietitians (RDs) promote nutrition practices and policies and can influence food consumption patterns to include nutrient dense foods such as beans. Although many evidence-based health benefits of bean consumption (e.g., cholesterol reduction, glycemic control) have been demonstrated, there is limited research on the knowledge, attitudes, and perceptions of RDs regarding the inclusion of beans in a healthy diet. To fill this existing research gap, this cross-sectional survey explored the perceptions, knowledge, and attitudes of 296 RDs in Arizona, USA, toward beans. The RDs largely held positive attitudes toward the healthfulness of beans and were aware of many health ...


Knowledge Gaps Of The Health Benefits Of Beans Among Low-Income Women, Shelly M. Palmer, Donna Winham, Christine A. Hradek Mph Jan 2018

Knowledge Gaps Of The Health Benefits Of Beans Among Low-Income Women, Shelly M. Palmer, Donna Winham, Christine A. Hradek Mph

Food Science and Human Nutrition Publications

Objectives: We determined knowledge of the health benefits of consuming beans, and assessed if awareness varied by acculturation status among Hispanic and non-Hispanic low-income women. Methods: We used a self-administered survey with Iowa women aged 18-65 years who were eligible to receive income-based services through 2 healthcare clinics, a WIC clinic, and Extension Outreach. Chi-square and ANOVA were used to compare bean health benefit knowledge, demographics, health-risk factors, nutrition information seeking, and self-efficacy by acculturation categories. Results: Of the 158 women who completed the survey, 58% were Hispanic, with a mean age of 36 years. In terms of acculturation, 24 ...


Ethanol Production By Soy Fiber Treatment And Simultaneous Saccharification And Co-Fermentation In An Integrated Corn-Soy Biorefinery, Jasreen K. Sekhon, Devin Maurer, Tong Wang, Stéphanie Jung, Kurt A. Rosentrater Jan 2018

Ethanol Production By Soy Fiber Treatment And Simultaneous Saccharification And Co-Fermentation In An Integrated Corn-Soy Biorefinery, Jasreen K. Sekhon, Devin Maurer, Tong Wang, Stéphanie Jung, Kurt A. Rosentrater

Food Science and Human Nutrition Publications

Insoluble fiber (IF) recovered from the enzyme-assisted aqueous extraction process (EAEP) of soybeans is a fraction rich in carbohydrates and proteins. It can be used to enhance ethanol production in an integrated corn-soy biorefinery, which combines EAEP with traditional corn-based ethanol processing. The present study evaluated IF as a substrate for ethanol production. The effects of treatment of IF (soaking in aqueous ammonia (SAA), liquid hot water (LHW), and enzymatic hydrolysis), primarily simultaneous saccharification and co-fermentation (SSCF), as well as scaling up (250 mL to 60 L) on ethanol production from IF alone or a corn and IF slurry were ...


Use Of Surfactant And Enzymes In Dry-Grind Corn Ethanol Fermentation Improves Yield Of Ethanol And Distillers Corn Oil, Lei Fang, Tong Wang Professor, Buddhi P. Lamsal Jan 2018

Use Of Surfactant And Enzymes In Dry-Grind Corn Ethanol Fermentation Improves Yield Of Ethanol And Distillers Corn Oil, Lei Fang, Tong Wang Professor, Buddhi P. Lamsal

Food Science and Human Nutrition Publications

Distillers corn oil (DCO) is a valuable co-product of dry-grind corn ethanol process. It can be used for bio-fuel production and in animal feed. DCO can be present in different forms in the fermentation matrix, including oil adhering to solid surfaces such as cell wall and protein matrix, and oil contained in unbroken cells, which is difficult to partition to thin stillage by decanting. Effects of using surfactant (Tween® 80) and enzymes during fermentation on DCO partition to thin stillage and DCO recovery from the condensed corn distillers with solubles (CCDS) were investigated. There was more than 8 ∼ 10% DCO ...


Characteristics, Functional Properties, And Antioxidant Activities Of Water-Soluble Proteins Extracted From Grasshoppers, Patanga Succincta And Chondracris Roseapbrunner, Niphattha Chatsuwan, Sitthipong Nalinanon, Yuporn Puechkamut, Buddhi P. Lamsal, Praphan Pinsirodom Jan 2018

Characteristics, Functional Properties, And Antioxidant Activities Of Water-Soluble Proteins Extracted From Grasshoppers, Patanga Succincta And Chondracris Roseapbrunner, Niphattha Chatsuwan, Sitthipong Nalinanon, Yuporn Puechkamut, Buddhi P. Lamsal, Praphan Pinsirodom

Food Science and Human Nutrition Publications

Water-soluble proteins extracted from two species of grasshoppers, Patanga succincta (WSPP) and Chondracris roseapbrunner (WSPC), were characterized as well as their functional properties and antioxidant activities were investigated. The extraction yield, on a wet weight basis, was 7.35% and 7.46% for WSPP and WSPC, respectively. The most abundant amino acid in both proteins was glutamic acid, followed by aspartic, alanine, and leucine, in that order. The electrophoretic study revealed that proteins with MW of 29, 42, 50, 69, and 146 kDa were the major protein components in WSPP and WSPC. FTIR analysis showed that those proteins remained their ...