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Food Science

University of Tennessee, Knoxville

Detection

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Full-Text Articles in Life Sciences

Novel Rapid Molecular Detection And Processing Approaches For The Control Of Salmonella Enterica Serovars In The Food Environment, Chayapa Techathuvanan May 2012

Novel Rapid Molecular Detection And Processing Approaches For The Control Of Salmonella Enterica Serovars In The Food Environment, Chayapa Techathuvanan

Doctoral Dissertations

The increase in Salmonella enterica outbreaks calls for an urgent need to rapidly detect and control Salmonella-associated contamination. Loop-mediated isothermal amplification (LAMP) assay is a novel method that can be completed within 90 min in a simple waterbath. Detection is by simple turbidity, fluorescence, or gel electrophoresis and is more specific than PCR. Reverse-transcriptase LAMP (RT-LAMP) targeting mRNA for the potential detection of live infectious Salmonella or recent contamination was used in this study and detection sensitivity to culture-based detection and RT-PCR assays was compared in pure culture, food products, and food processing environments. Our results showed detection limits ...


Novel Approaches For The Efficient Sampling And Detection Of Listeria Monocytogenes And Brochothrix Thermosphacta On Food Contact Surfaces, Jessica Anne Clemons Dec 2010

Novel Approaches For The Efficient Sampling And Detection Of Listeria Monocytogenes And Brochothrix Thermosphacta On Food Contact Surfaces, Jessica Anne Clemons

Masters Theses

The primary step in the microbiological assessment of highly dynamic and complex food processing conditions is environmental sampling. The objectives of this study were to: (1) compare the efficacy of four sampling devices including Microbial-Vac system (MV), cellulose sponge (SP), polyester swab (SW) and composite tissue (CT), for the recovery of Listeria monocytogenes and Brochothrix thermosphacta on five surfaces and (2) to determine if there was a significant difference between the recovery of low (10 CFU/900cm2) and high (100 CFU/900cm2) L. monocytogenes inoculum levels using the sampling devices in a simulated food processing environment. Surfaces used ...