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Full-Text Articles in Life Sciences

Isolation, Antibiotic Resistance, And Molecular Characterization Of Campylobacter From Poultry, Swine And Dairy Cows, Willie James Taylor Dec 2012

Isolation, Antibiotic Resistance, And Molecular Characterization Of Campylobacter From Poultry, Swine And Dairy Cows, Willie James Taylor

Doctoral Dissertations

This study was conducted to determine presence, antibiotic resistance, and genetic relatedness of Campylobacter on diverse farm types. Cloacal fecal samples from poultry (broilers, layers, and turkey breeders), and fecal rectal samples from non-poultry animals (farrowing sows, finisher pigs, and lactating dairy cows) were tested for Campylobacter using BAM protocols. Agar disk diffusion method was used to determine antibiotic resistance, and PFGE analysis to determine genetic relatedness of isolates recovered within the different farm types. Campylobacter was detected in 34.8% (range of 6.7 to 62.0%, P < 0.001) of cloacal samples from poultry, in 61.4% (range of 55.0 to 69.0%, P < 0.05), and 26.0% (range of 19.0 to 34.0%, P < 0.05) of rectal samples from pigs, and dairy cows, respectively. Antibiotic resistance was detected in 30.2% (range of ...


Casein Micelles From Bovine Milk: Native Structure, Interactions, And Practical Applications Of Their Structural Modification, Raymundo Trejo Dec 2012

Casein Micelles From Bovine Milk: Native Structure, Interactions, And Practical Applications Of Their Structural Modification, Raymundo Trejo

Doctoral Dissertations

A definitive structure of the native casein micelle structure continues to elude researchers. Data and images obtained via cryo - transmission electron microscopy of isolated native casein micelles allowed for the reconstruction of a three dimensional model of the micelle; which contains water filled cavities (ca. 20 to 30 nm diameter), channels (diameter larger than ca. 5 nm), and several hundred high density nanoclusters (6-12 nm diameter) within the interior of the micelles.

The SDS polyacrylamide gel electrophoresis of isolated micelles (pH 6.8, 5.0) showed that whey proteins were found associated with the isolated micelles regardless of the pH ...


Impact Of Rearing Conditions On The Microbiological Quality Of Retail Poultry Meat, Bridgshe Hardy Dec 2012

Impact Of Rearing Conditions On The Microbiological Quality Of Retail Poultry Meat, Bridgshe Hardy

Masters Theses

Poultry and poultry products are a leading source of foodborne pathogens and illnesses. The rearing conditions of poultry can be an influential factor on the presence of foodborne pathogens including Campylobacter and Salmonella because some types of rearing practices have increased risks in terms of biosecurity. However, there is a gap in knowledge of food safety in raw chicken products and no studies have reported the microbiological quality of turkeys produced under different rearing environments. Thus, the aim of this study was to compare conventionally and organically-reared whole chicken and turkey carcasses purchased from three retail outlets in Knoxville, TN ...


Sp746-B Good Agricultural Practices (Gap) Certification In Tennessee, Faith Critzer, Annette Wszelaki Nov 2012

Sp746-B Good Agricultural Practices (Gap) Certification In Tennessee, Faith Critzer, Annette Wszelaki

Food, Nutrition and Food Safety

No abstract provided.


Sp737 Efnep Partner Brochure, Janie Burney Nov 2012

Sp737 Efnep Partner Brochure, Janie Burney

Food, Nutrition and Food Safety

Expanded Food and Nutrition Education Program information for program partners


Changes In Carbohydrates Of Navy Beans During Hydration And Subsequent Thermal Processing, Robert Blake Lowe Aug 2012

Changes In Carbohydrates Of Navy Beans During Hydration And Subsequent Thermal Processing, Robert Blake Lowe

Masters Theses

Navy beans were subjected to six different hydration protocols that varied in time, temperature, and methodology after which they were thermally processed in both a brine solution and a typical baked bean sauce. Beans, isolated starch, and hydration fluid were analyzed immediately after the completion of hydration protocols. Significant differences were noted between those protocols utilizing low heat (1 and 2) and those utilizing high heat methods (3-6). Bean from low heat protocols were firmer overall. Starch granules from low heat beans were smaller in overall size, experienced less surface damage retained birefringence, and could absorb more water (higher swell ...


Volume 9, Number 1 (Spring/Summer 2012), Ut Institute Of Agriculture Jul 2012

Volume 9, Number 1 (Spring/Summer 2012), Ut Institute Of Agriculture

Tennessee Land, Life and Science Magazine

Issue Highlights:

  • UT Veterinary Medicine Center Treats Smokey
  • Farm Credit Services Creates Top Scholars Program
  • Perspective on the Importance and Future of the Institute


Novel Rapid Molecular Detection And Processing Approaches For The Control Of Salmonella Enterica Serovars In The Food Environment, Chayapa Techathuvanan May 2012

Novel Rapid Molecular Detection And Processing Approaches For The Control Of Salmonella Enterica Serovars In The Food Environment, Chayapa Techathuvanan

Doctoral Dissertations

The increase in Salmonella enterica outbreaks calls for an urgent need to rapidly detect and control Salmonella-associated contamination. Loop-mediated isothermal amplification (LAMP) assay is a novel method that can be completed within 90 min in a simple waterbath. Detection is by simple turbidity, fluorescence, or gel electrophoresis and is more specific than PCR. Reverse-transcriptase LAMP (RT-LAMP) targeting mRNA for the potential detection of live infectious Salmonella or recent contamination was used in this study and detection sensitivity to culture-based detection and RT-PCR assays was compared in pure culture, food products, and food processing environments. Our results showed detection limits ...


Plant Protein-Based Nanocomposite Materials: Modification Of Layered Nanoclay By Surface Coating And Enhanced Interactions By Enzymatic And Chemical Cross-Linking, Minfeng Jin May 2012

Plant Protein-Based Nanocomposite Materials: Modification Of Layered Nanoclay By Surface Coating And Enhanced Interactions By Enzymatic And Chemical Cross-Linking, Minfeng Jin

Doctoral Dissertations

Highly intercalated or exfoliated nanoclay montmorillonite (MMT) has promises to improve mechanical and barrier properties of nanocomposite materials that may be further improved by strengthening interactions between matrix polymers and nanofillers. In this work, water-soluble proteins extracted from hominy feed and soy flour were utilized to modify the structures of MMT layers by surface-coating. Following coating at 60 °C using different MMT:protein mass ratios (49:1-2:1) and pH (2.0-10.0), the nanoclay was triple-washed for zeta potential analysis and lyophilized for X-ray diffraction and Fourier transform infrared spectroscopy analyses. Results showed that protein adsorption on MMT occurred ...


Chenopodium Berlandieri And The Cultural Origins Of Agriculture In The Eastern Woodlands, Daniel Shelton Robinson May 2012

Chenopodium Berlandieri And The Cultural Origins Of Agriculture In The Eastern Woodlands, Daniel Shelton Robinson

Masters Theses

The development of agriculture in the New World has been a topic of prominent historic interest, but one that has ignored some regions in favor of others. The woodlands of Eastern North America have felt this bias in the investigation of agricultural origins, but this has not prevented the development of theories to explain the emergence of a complex of indigenous agricultural plants in the region. Data collection and technological advances have in large part validated these theories, creating a model for domestication. By emphasizing farming over other cultural practices, however, these theories lack explanatory power with regards to the ...


Chitosan-Gallic Acid Films As Multifunctional Food Packaging, Stephanie Beth Schreiber May 2012

Chitosan-Gallic Acid Films As Multifunctional Food Packaging, Stephanie Beth Schreiber

Masters Theses

Chitosan is a good candidate for multifunctional food packaging because of its biocompatibility, biodegradability, antibacterial properties, secondary antioxidant activity, film forming ability, resistance to lipids and because of its structure which is very desirable for grafting various compounds to it. For this research, we took advantage of chitosan’s amino group that has nuceleophilic character at a pH above its pKa, which is 6.3. Gallic acid, a phenolic compound with primary antioxidant properties was grafted to chitosan using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide. Grafting was evaluated using FTIR-ATR and 1H and 13CNMR. FTIR showed evidence of grafting ...


Volume 9, Number 2 (Fall/Winter 2012), Ut Institute Of Agriculture Jan 2012

Volume 9, Number 2 (Fall/Winter 2012), Ut Institute Of Agriculture

Tennessee Land, Life and Science Magazine

Issue Highlights:

  • The Plight of Pollinators: Working to Sustain Our Food Supply
  • Celebrating a Land-Grant Milestone
  • UT Researchers Devise New Precision Ag Technology