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Bioresource and Agricultural Engineering

Selected Works

2008

Chenxu Yu

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Full-Text Articles in Life Sciences

Raman Multiplexers For Alternative Gene Splicing, Lan Sun, Chenxu Yu, Joseph Irudayaraj May 2008

Raman Multiplexers For Alternative Gene Splicing, Lan Sun, Chenxu Yu, Joseph Irudayaraj

Chenxu Yu

Nonfluorescent labels were used for an array-format multiplex detection of alternative splice junctions of breast cancer susceptibility gene 1 (BRCA1) by surface-enhanced Raman scattering (SERS). A four-plex detection scheme using nonfluorescent labels was demonstrated for DNA sequences specific to four BRCA1 alternative splice variants:  Δ(11q) (the last 3309 nt deleted from exon 11), Δ(9, 10) (exon 9 and 10 deleted), Δ(5) (exon 5 deleted), and Δ(5q, 6) (the last 22 nt of exon 5 and the entire exon 6 is deleted). This is the first proof-of-concept study to apply SERS-based detection using nonfluorescent labels to investigate ...