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GPU deconvolution

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Gadph Single Molecule Mrna Fish 20141104tue, George Mcnamara Nov 2014

Gadph Single Molecule Mrna Fish 20141104tue, George Mcnamara

George McNamara

GADPH single molecule mRNA FISH 20141104Tue

Raw data on NVidia TITAN GPU card deconvolution using original Bruce and Butte 2013 Optics Express software running inside Fiji ImageJ.

B&B GPU Deconvolution Reference:

http://www.opticsinfobase.org/oe/fulltext.cfm?uri=oe-21-4-4766&id=249375

Acquired at 63x1x using leica plan apo 1.4 NA objective lens on Cooper lab Leica DMI6000 microscope, SOLA (initial release) LED lamp using only "green" LED, 554x Semrock exciter filter (in ASI ex wheel), Leica N3 (Cy3) filter cube, center 600 nm emission wavelength.

Hamamatsu FLASH4.0 sCMOS, 100 ms exposure time so 6.4 seconds ...


More Fish (1 Of 4), George Mcnamara Mar 2014

More Fish (1 Of 4), George Mcnamara

George McNamara

More Stellaris FRISH and GPU Deconvolution, 20140313Thur - part 1 of 4

Please see other content in the FISH Imaging section of

http://0-works.bepress.com.library.simmons.edu/gmcnamara/subject_areas.html

for details on the microscope. Briefly: Leica DMI6000 inverted microscope, SOLA lamp, Hamamatsu FLASH4.0 sCMOS camera, 100 nm XY, 200 nm Z pixels, 32 planes (16 um Z), MetaMorph 7.8 acquisition software.

Four zip files:

http://0-works.bepress.com.library.simmons.edu/gmcnamara/43

http://0-works.bepress.com.library.simmons.edu/gmcnamara/44

http://0-works.bepress.com.library.simmons.edu/gmcnamara/45

http://0-works.bepress.com.library.simmons.edu/gmcnamara/46

with two dataset (001, 002), raw and deconvolved using Bruce & Butte ...


More Fish (2 Of 4), George Mcnamara Mar 2014

More Fish (2 Of 4), George Mcnamara

George McNamara

More Stellaris FISH and GPU Deconvolution, 20140313Thur - part 2 of 4

Please see other content in the FISH Imaging section of

http://0-works.bepress.com.library.simmons.edu/gmcnamara/subject_areas.html

for details on the microscope. Briefly: Leica DMI6000 inverted microscope, SOLA lamp, Hamamatsu FLASH4.0 sCMOS camera, 100 nm XY, 200 nm Z pixels, 32 planes (16 um Z), MetaMorph 7.8 acquisition software.

Four zip files:

http://0-works.bepress.com.library.simmons.edu/gmcnamara/43

http://0-works.bepress.com.library.simmons.edu/gmcnamara/44

http://0-works.bepress.com.library.simmons.edu/gmcnamara/45

http://0-works.bepress.com.library.simmons.edu/gmcnamara/46

with two dataset (001, 002), raw and deconvolved using Bruce & Butte ...


More Fish (3 Of 4), George Mcnamara Mar 2014

More Fish (3 Of 4), George Mcnamara

George McNamara

More Stellaris FISH and GPU Deconvolution, 20140313Thur - part 3 of 4

Please see other content in the FISH Imaging section of

http://0-works.bepress.com.library.simmons.edu/gmcnamara/subject_areas.html

for details on the microscope. Briefly: Leica DMI6000 inverted microscope, SOLA lamp, Hamamatsu FLASH4.0 sCMOS camera, 100 nm XY, 200 nm Z pixels, 32 planes (16 um Z), MetaMorph 7.8 acquisition software.

Four zip files:

http://0-works.bepress.com.library.simmons.edu/gmcnamara/43

http://0-works.bepress.com.library.simmons.edu/gmcnamara/44

http://0-works.bepress.com.library.simmons.edu/gmcnamara/45

http://0-works.bepress.com.library.simmons.edu/gmcnamara/46

with two dataset (001, 002), raw and deconvolved using Bruce & Butte ...


More Fish (4 Of 4), George Mcnamara Mar 2014

More Fish (4 Of 4), George Mcnamara

George McNamara

More Stellaris FISH and GPU Deconvolution, 20140313Thur - part 4 of 4

Please see other content in the FISH Imaging section of

http://0-works.bepress.com.library.simmons.edu/gmcnamara/subject_areas.html

for details on the microscope. Briefly: Leica DMI6000 inverted microscope, SOLA lamp, Hamamatsu FLASH4.0 sCMOS camera, 100 nm XY, 200 nm Z pixels, 32 planes (16 um Z), MetaMorph 7.8 acquisition software.

Four zip files:

http://0-works.bepress.com.library.simmons.edu/gmcnamara/43

http://0-works.bepress.com.library.simmons.edu/gmcnamara/44

http://0-works.bepress.com.library.simmons.edu/gmcnamara/45

http://0-works.bepress.com.library.simmons.edu/gmcnamara/46

with two dataset (001, 002), raw and deconvolved using Bruce & Butte ...


Stellaris Fish Workshop Gadph And Dapi Z-Series, George Mcnamara Dec 2013

Stellaris Fish Workshop Gadph And Dapi Z-Series, George Mcnamara

George McNamara

Stellaris FISH workshop GADPH and DAPI Z-series at MD Anderson Cancer Center, Houston, TX.

The zip file contains raw and GPU deconvolved image data from a workshop Biosearch Technologies conducted for MDACC researchers in December 2013. Image data was acquired on a Leica DMI6000 microscope with Lumencor SOLA light engine, DAPI and Cy5 filter cubes, Hamamatsu ORCA FLASH4.0 sCMOS camera (500 ms exposure time per plane for Quasar 670).

Pixel size 100 nm XY.

Z-step size 200 nm.

32 planes (power of 2 is optimal for GPU deconvolution). With 500 ms exposure time, the Quasar 670 GADPH FISH probes ...