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More Fish (1 Of 4), George Mcnamara Mar 2014

More Fish (1 Of 4), George Mcnamara

George McNamara

More Stellaris FRISH and GPU Deconvolution, 20140313Thur - part 1 of 4

Please see other content in the FISH Imaging section of

http://0-works.bepress.com.library.simmons.edu/gmcnamara/subject_areas.html

for details on the microscope. Briefly: Leica DMI6000 inverted microscope, SOLA lamp, Hamamatsu FLASH4.0 sCMOS camera, 100 nm XY, 200 nm Z pixels, 32 planes (16 um Z), MetaMorph 7.8 acquisition software.

Four zip files:

http://0-works.bepress.com.library.simmons.edu/gmcnamara/43

http://0-works.bepress.com.library.simmons.edu/gmcnamara/44

http://0-works.bepress.com.library.simmons.edu/gmcnamara/45

http://0-works.bepress.com.library.simmons.edu/gmcnamara/46

with two dataset (001, 002), raw and deconvolved using Bruce & Butte ...


More Fish (2 Of 4), George Mcnamara Mar 2014

More Fish (2 Of 4), George Mcnamara

George McNamara

More Stellaris FISH and GPU Deconvolution, 20140313Thur - part 2 of 4

Please see other content in the FISH Imaging section of

http://0-works.bepress.com.library.simmons.edu/gmcnamara/subject_areas.html

for details on the microscope. Briefly: Leica DMI6000 inverted microscope, SOLA lamp, Hamamatsu FLASH4.0 sCMOS camera, 100 nm XY, 200 nm Z pixels, 32 planes (16 um Z), MetaMorph 7.8 acquisition software.

Four zip files:

http://0-works.bepress.com.library.simmons.edu/gmcnamara/43

http://0-works.bepress.com.library.simmons.edu/gmcnamara/44

http://0-works.bepress.com.library.simmons.edu/gmcnamara/45

http://0-works.bepress.com.library.simmons.edu/gmcnamara/46

with two dataset (001, 002), raw and deconvolved using Bruce & Butte ...


More Fish (3 Of 4), George Mcnamara Mar 2014

More Fish (3 Of 4), George Mcnamara

George McNamara

More Stellaris FISH and GPU Deconvolution, 20140313Thur - part 3 of 4

Please see other content in the FISH Imaging section of

http://0-works.bepress.com.library.simmons.edu/gmcnamara/subject_areas.html

for details on the microscope. Briefly: Leica DMI6000 inverted microscope, SOLA lamp, Hamamatsu FLASH4.0 sCMOS camera, 100 nm XY, 200 nm Z pixels, 32 planes (16 um Z), MetaMorph 7.8 acquisition software.

Four zip files:

http://0-works.bepress.com.library.simmons.edu/gmcnamara/43

http://0-works.bepress.com.library.simmons.edu/gmcnamara/44

http://0-works.bepress.com.library.simmons.edu/gmcnamara/45

http://0-works.bepress.com.library.simmons.edu/gmcnamara/46

with two dataset (001, 002), raw and deconvolved using Bruce & Butte ...


More Fish (4 Of 4), George Mcnamara Mar 2014

More Fish (4 Of 4), George Mcnamara

George McNamara

More Stellaris FISH and GPU Deconvolution, 20140313Thur - part 4 of 4

Please see other content in the FISH Imaging section of

http://0-works.bepress.com.library.simmons.edu/gmcnamara/subject_areas.html

for details on the microscope. Briefly: Leica DMI6000 inverted microscope, SOLA lamp, Hamamatsu FLASH4.0 sCMOS camera, 100 nm XY, 200 nm Z pixels, 32 planes (16 um Z), MetaMorph 7.8 acquisition software.

Four zip files:

http://0-works.bepress.com.library.simmons.edu/gmcnamara/43

http://0-works.bepress.com.library.simmons.edu/gmcnamara/44

http://0-works.bepress.com.library.simmons.edu/gmcnamara/45

http://0-works.bepress.com.library.simmons.edu/gmcnamara/46

with two dataset (001, 002), raw and deconvolved using Bruce & Butte ...


Time Series Data For 3b Image Processing, George Mcnamara Jan 2014

Time Series Data For 3b Image Processing, George Mcnamara

George McNamara

Time series data for 3B image processing

Four time series data sets of Stellaris single molecules RNA FISH (fluorescence in situ hybridization). All four datasets are 301 imaqe planes. FISH probes are to TOP1 mRNA (Topoisomerase I)in Saos osterosarcoma cells.

One dataset is 10 millisecond exposures (very dim), acquired in Streaming acquisition mode (no hardware overhead).

The other three datasets are a three consecutive planes of the same XY field of view.

I have included image processing results for:

PiMP - a fast method developed by Sebastian Munck et al 2012, http://www.ncbi.nlm.nih.gov/pubmed/?term=22357945 ...