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Full-Text Articles in Life Sciences

A Partnership Approach To Protecting And Improving Water Quality In The Shellfish Watersheds Of Pierce County, Cindy Callahan, Erin Ewald, Barbara Ann Smolko, Ray Hanowell May 2014

A Partnership Approach To Protecting And Improving Water Quality In The Shellfish Watersheds Of Pierce County, Cindy Callahan, Erin Ewald, Barbara Ann Smolko, Ray Hanowell

Salish Sea Ecosystem Conference

Commercial shellfish growing is a significant economic driver on the Key Peninsula of Pierce County, Washington. In response to the downgrades of several shellfish areas in the 1990’s and early 2000’s and the establishment of Shellfish Protection Districts and Closure Response Plans, it was recognized that a more proactive approach was needed. In an effort to build cooperation and a partnership across Pierce County, and address bacterial contamination in the shellfish growing areas, a Pierce County Shellfish Partners Strategic Plan was developed and formalized in March of 2013. The partnership initially included Pierce County Surface Water Management (SWM ...


P-19 Hcnabc Operon Transcription Of Pseudomonas Putida Under Varying Iron And Oxygen Concentrations And Culture Age, Christine Lee Mar 2014

P-19 Hcnabc Operon Transcription Of Pseudomonas Putida Under Varying Iron And Oxygen Concentrations And Culture Age, Christine Lee

Honors Scholars & Undergraduate Research Poster Symposium Programs

The species Pseudomonas putida produces hydrogen cyanide (HCN) through the transcription of the hcnABC operon. The purpose of this experiment is to determine how the hcnABC operon is affected by varying levels of iron and oxygen, and age of bacteria culture. To test this, P. putida is grown under five conditions: the presence of iron, the absence of iron, with aeration, without aeration, and without iron and aeration. At 8, 18, and 30 hours, the cultures are assessed for HCN production and cell density. HCN production is measured via bioluminescence; light emission occurs whenever HCN is produced because the modified ...