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Timelapse Data - Gm Imaging Cytometer Needs Stitching Part 2 Of 2, George Mcnamara Jul 2014

Timelapse Data - Gm Imaging Cytometer Needs Stitching Part 2 Of 2, George Mcnamara

George McNamara

Timelapse data - GM imaging cytometer needs stitching part 2 of 2

Imaging challenge: align multiple time series over time to overcome "stage slop" in the imaging cytometer used in this experiment.

http://0-works.bepress.com.library.simmons.edu/gmcnamara/53 (previous) and http://0-works.bepress.com.library.simmons.edu/gmcnamara/54 (this)

are zip files containing 8-bit stacks (MetaMorph multiplane TIFF files) acquired on an imaging cytometer.

"53" is the brightfield data, raw and automatic aligned stacks in MetaMorph (Apps menu, MM7.8.6). "54" is GFP fluorescence, raw and automatic aligned stacks in MetaMorph (Apps menu, MM7.8.6). The GFP fluorescence decreases over time ...


An Interview At Toward A Science Of Consciousness In Tuscon, Arizona. Usa 2014, Soam Prakash Jun 2014

An Interview At Toward A Science Of Consciousness In Tuscon, Arizona. Usa 2014, Soam Prakash

Soam Prakash

No abstract provided.


Pubspectra To Do Data, George Mcnamara May 2014

Pubspectra To Do Data, George Mcnamara

George McNamara

PubSpectra raw data that I had in my "to do" folder to add to the PubSpectra data set but never did.

PubSpectra has over 2000 spectra in it (last updated 2006) in Excel "big file" format - the XLSX file inside the zip file at

http://0-works.bepress.com.library.simmons.edu/gmcnamara/9/

Anyone is welcome to add and organize new data yourselves. I used Un-Scan-It from Silk Scientific (www.silkscientific.com).

More importantly, I strongly adopting my PubSpectra format of 1 nm wavelength intervals, with the row number corresponding to nm. For example, 400 nm data appears in row 400.

Much of ...


Jibin Zhang's Ppt For Midwest Meeting.Pdf, Jibin Zhang, Yeunsu Suh, Young Min Choi, Michael E. Davis, Kichoon Lee Mar 2014

Jibin Zhang's Ppt For Midwest Meeting.Pdf, Jibin Zhang, Yeunsu Suh, Young Min Choi, Michael E. Davis, Kichoon Lee

Jibin Zhang

Increasing numbers of fat cells in adipose tissue are attributed to proliferation of preadipocytes or immature adipocytes in the early stages as well as adipogenic differentiation in the later stages of adipogenesis. Although both events are involved in the increase in fat cell numbers, they are contrary to each other in that the former requires cell cycle activity whereas the latter requires cell cycle withdrawal. Therefore, appropriate regulation of cell cycle inhibition is critical to adipogenesis.
To explore the important cell cycle inhibitors and study their expression in adipogenesis, a strategy was adopted that combined the Gene Expression Omnibus (GEO ...


Dengue Virus-Specific Human Cd4+ T-Lymphocyte Responses In A Recipient Of An Experimental Live-Attenuated Dengue Virus Type 1 Vaccine: Bulk Culture Proliferation, Clonal Analysis, And Precursor Frequency Determination, Sharone Green, Ichiro Kurane, Robert Edelman, Carol 0. Tacket, Kenneth H. Eckels, David W. Vaughn, Charles H. Hoke, Francis A. Ennis Jan 2014

Dengue Virus-Specific Human Cd4+ T-Lymphocyte Responses In A Recipient Of An Experimental Live-Attenuated Dengue Virus Type 1 Vaccine: Bulk Culture Proliferation, Clonal Analysis, And Precursor Frequency Determination, Sharone Green, Ichiro Kurane, Robert Edelman, Carol 0. Tacket, Kenneth H. Eckels, David W. Vaughn, Charles H. Hoke, Francis A. Ennis

Sharone Green

We analyzed the CD4+ T-lymphocyte responses to dengue, West Nile, and yellow fever viruses 4 months after immunization of a volunteer with an experimental live-attenuated dengue virus type 1 vaccine (DEN-1 45AZ5). We examined bulk culture proliferation to noninfectious antigens, determined the precursor frequency of specific CD4+ T cells by limiting dilution, and established and analyzed CD4+ T-cell clones. Bulk culture proliferation was predominantly dengue virus type 1 specific with a lesser degree of cross-reactive responses to other dengue virus serotypes, West Nile virus, and yellow fever virus. Precursor frequency determination by limiting dilution in the presence of noninfectious dengue ...