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2014

Andrews University

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Full-Text Articles in Life Sciences

P-19 Hcnabc Operon Transcription Of Pseudomonas Putida Under Varying Iron And Oxygen Concentrations And Culture Age, Christine Lee Mar 2014

P-19 Hcnabc Operon Transcription Of Pseudomonas Putida Under Varying Iron And Oxygen Concentrations And Culture Age, Christine Lee

Honors Scholars & Undergraduate Research Poster Symposium Programs

The species Pseudomonas putida produces hydrogen cyanide (HCN) through the transcription of the hcnABC operon. The purpose of this experiment is to determine how the hcnABC operon is affected by varying levels of iron and oxygen, and age of bacteria culture. To test this, P. putida is grown under five conditions: the presence of iron, the absence of iron, with aeration, without aeration, and without iron and aeration. At 8, 18, and 30 hours, the cultures are assessed for HCN production and cell density. HCN production is measured via bioluminescence; light emission occurs whenever HCN is produced because the modified ...