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2014

Cell and Developmental Biology

Stellaris FISH

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Gadph Single Molecule Mrna Fish 20141104tue, George Mcnamara Nov 2014

Gadph Single Molecule Mrna Fish 20141104tue, George Mcnamara

George McNamara

GADPH single molecule mRNA FISH 20141104Tue

Raw data on NVidia TITAN GPU card deconvolution using original Bruce and Butte 2013 Optics Express software running inside Fiji ImageJ.

B&B GPU Deconvolution Reference:

http://www.opticsinfobase.org/oe/fulltext.cfm?uri=oe-21-4-4766&id=249375

Acquired at 63x1x using leica plan apo 1.4 NA objective lens on Cooper lab Leica DMI6000 microscope, SOLA (initial release) LED lamp using only "green" LED, 554x Semrock exciter filter (in ASI ex wheel), Leica N3 (Cy3) filter cube, center 600 nm emission wavelength.

Hamamatsu FLASH4.0 sCMOS, 100 ms exposure time so 6.4 seconds ...


More Fish (1 Of 4), George Mcnamara Mar 2014

More Fish (1 Of 4), George Mcnamara

George McNamara

More Stellaris FRISH and GPU Deconvolution, 20140313Thur - part 1 of 4

Please see other content in the FISH Imaging section of

http://0-works.bepress.com.library.simmons.edu/gmcnamara/subject_areas.html

for details on the microscope. Briefly: Leica DMI6000 inverted microscope, SOLA lamp, Hamamatsu FLASH4.0 sCMOS camera, 100 nm XY, 200 nm Z pixels, 32 planes (16 um Z), MetaMorph 7.8 acquisition software.

Four zip files:

http://0-works.bepress.com.library.simmons.edu/gmcnamara/43

http://0-works.bepress.com.library.simmons.edu/gmcnamara/44

http://0-works.bepress.com.library.simmons.edu/gmcnamara/45

http://0-works.bepress.com.library.simmons.edu/gmcnamara/46

with two dataset (001, 002), raw and deconvolved using Bruce & Butte ...


More Fish (2 Of 4), George Mcnamara Mar 2014

More Fish (2 Of 4), George Mcnamara

George McNamara

More Stellaris FISH and GPU Deconvolution, 20140313Thur - part 2 of 4

Please see other content in the FISH Imaging section of

http://0-works.bepress.com.library.simmons.edu/gmcnamara/subject_areas.html

for details on the microscope. Briefly: Leica DMI6000 inverted microscope, SOLA lamp, Hamamatsu FLASH4.0 sCMOS camera, 100 nm XY, 200 nm Z pixels, 32 planes (16 um Z), MetaMorph 7.8 acquisition software.

Four zip files:

http://0-works.bepress.com.library.simmons.edu/gmcnamara/43

http://0-works.bepress.com.library.simmons.edu/gmcnamara/44

http://0-works.bepress.com.library.simmons.edu/gmcnamara/45

http://0-works.bepress.com.library.simmons.edu/gmcnamara/46

with two dataset (001, 002), raw and deconvolved using Bruce & Butte ...


More Fish (3 Of 4), George Mcnamara Mar 2014

More Fish (3 Of 4), George Mcnamara

George McNamara

More Stellaris FISH and GPU Deconvolution, 20140313Thur - part 3 of 4

Please see other content in the FISH Imaging section of

http://0-works.bepress.com.library.simmons.edu/gmcnamara/subject_areas.html

for details on the microscope. Briefly: Leica DMI6000 inverted microscope, SOLA lamp, Hamamatsu FLASH4.0 sCMOS camera, 100 nm XY, 200 nm Z pixels, 32 planes (16 um Z), MetaMorph 7.8 acquisition software.

Four zip files:

http://0-works.bepress.com.library.simmons.edu/gmcnamara/43

http://0-works.bepress.com.library.simmons.edu/gmcnamara/44

http://0-works.bepress.com.library.simmons.edu/gmcnamara/45

http://0-works.bepress.com.library.simmons.edu/gmcnamara/46

with two dataset (001, 002), raw and deconvolved using Bruce & Butte ...


More Fish (4 Of 4), George Mcnamara Mar 2014

More Fish (4 Of 4), George Mcnamara

George McNamara

More Stellaris FISH and GPU Deconvolution, 20140313Thur - part 4 of 4

Please see other content in the FISH Imaging section of

http://0-works.bepress.com.library.simmons.edu/gmcnamara/subject_areas.html

for details on the microscope. Briefly: Leica DMI6000 inverted microscope, SOLA lamp, Hamamatsu FLASH4.0 sCMOS camera, 100 nm XY, 200 nm Z pixels, 32 planes (16 um Z), MetaMorph 7.8 acquisition software.

Four zip files:

http://0-works.bepress.com.library.simmons.edu/gmcnamara/43

http://0-works.bepress.com.library.simmons.edu/gmcnamara/44

http://0-works.bepress.com.library.simmons.edu/gmcnamara/45

http://0-works.bepress.com.library.simmons.edu/gmcnamara/46

with two dataset (001, 002), raw and deconvolved using Bruce & Butte ...