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2012

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Articles 1 - 13 of 13

Full-Text Articles in Life Sciences

Regulation Of Atg4b Stability By Rnf5 Limits Basal Levels Of Autophagy And Influences Susceptibility To Bacterial Infection, E. Kuang, Cheryl Okumura, S. Sheffy-Levin, T. Varsano, V. Shu, J. Qi, I. Niesman, H. Yang, C. LóPez-OtíN, W. Yang, J. Reed, L. Broday, V. Nizet, Z. Ronai Oct 2012

Regulation Of Atg4b Stability By Rnf5 Limits Basal Levels Of Autophagy And Influences Susceptibility To Bacterial Infection, E. Kuang, Cheryl Okumura, S. Sheffy-Levin, T. Varsano, V. Shu, J. Qi, I. Niesman, H. Yang, C. LóPez-OtíN, W. Yang, J. Reed, L. Broday, V. Nizet, Z. Ronai

Cheryl Okumura

Autophagy is the mechanism by which cytoplasmic components and organelles are degraded by the lysosomal machinery in response to diverse stimuli including nutrient deprivation, intracellular pathogens, and multiple forms of cellular stress. Here, we show that the membrane-associated E3 ligase RNF5 regulates basal levels of autophagy by controlling the stability of a select pool of the cysteine protease ATG4B. RNF5 controls the membranal fraction of ATG4B and limits LC3 (ATG8) processing, which is required for phagophore and autophagosome formation. The association of ATG4B with—and regulation of its ubiquitination and stability by—RNF5 is seen primarily under normal growth conditions ...


Halloween 2012 Jack O'Lanterns Trick Or Treat, George Mcnamara Oct 2012

Halloween 2012 Jack O'Lanterns Trick Or Treat, George Mcnamara

George McNamara

Halloween 2012 makes trick or treating more visual and interactive than in past years.

the download is a ZIP file containing three files.

Print out the (unnumbered) image on as large and nice printer paper as possible - I used glossy 44" wide here in Miami (University of Miami, MillerSchool of Medicine, Calder Library, Biomedical Communications dept - I also made another print on "fabric", also 44" wide to take with me to an HHMI Janelia Farm conference on 'turning images into knowledge' that ends on Oct 31 - might stay up for a second conference, "GFP..." that start Nov 4).

The other ...


Animal Welfare In Aai, John New Oct 2012

Animal Welfare In Aai, John New

John C. New Jr., DVM, MPH, DACVPM

No abstract provided.


Mcnamara 20120831fri-20120904tue Cosmic Ray Particles By Ccd Imaging, George Mcnamara Sep 2012

Mcnamara 20120831fri-20120904tue Cosmic Ray Particles By Ccd Imaging, George Mcnamara

George McNamara

McNamara 20120831Fri-20120904Tue Cosmic Ray Particles by CCD imaging.zip contains image files in support of a Microscopy Today article - please see

http://www.microscopy-today.com/


A New Pharmacological Agent (Akb-4924) Stabilizes Hypoxia Inducible Factor (Hif) And Increases Skin Innate Defenses Against Bacterial Infection., Cheryl Okumura, A. Hollands, D. Tran, J. Olson, S. Dahesh, M. Von KöCkritz-Blickwede, W. Thienphrapa, C. Corle, S. Jeung, A. Kotsakis, R. Shalwitz, R. Johnson, V. Nizet Aug 2012

A New Pharmacological Agent (Akb-4924) Stabilizes Hypoxia Inducible Factor (Hif) And Increases Skin Innate Defenses Against Bacterial Infection., Cheryl Okumura, A. Hollands, D. Tran, J. Olson, S. Dahesh, M. Von KöCkritz-Blickwede, W. Thienphrapa, C. Corle, S. Jeung, A. Kotsakis, R. Shalwitz, R. Johnson, V. Nizet

Cheryl Okumura

Hypoxia inducible factor-1 (HIF-1) is a transcription factor that is a major regulator of energy homeostasis and cellular adaptation to low oxygen stress. HIF-1 is also activated in response to bacterial pathogens and supports the innate immune response of both phagocytes and keratinocytes. In this work, we show that a new pharmacological compound AKB-4924 (Akebia Therapeutics) increases HIF-1α levels and enhances the antibacterial activity of phagocytes and keratinocytes against both methicillin-sensitive and -resistant strains of Staphylococcus aureus in vitro. AKB-4924 is also effective in stimulating the killing capacity of keratinocytes against the important opportunistic skin pathogens Pseudomonas aeruginosa and Acinitobacter ...


Novel Role Of The Transcription Factor Hif-1Α In The Formation Of Mast Cell Extracellular Traps., K. Branitzki-Heinemann, Cheryl Okumura, L. VöLlger, Y. Kawakami, T. Kawakami, H. Naim, V. Nizet, M. Von KöCkritz-Blickwede Aug 2012

Novel Role Of The Transcription Factor Hif-1Α In The Formation Of Mast Cell Extracellular Traps., K. Branitzki-Heinemann, Cheryl Okumura, L. VöLlger, Y. Kawakami, T. Kawakami, H. Naim, V. Nizet, M. Von KöCkritz-Blickwede

Cheryl Okumura

MCs (mast cells) are critical components of the host innate immune defence against bacterial pathogens, providing a variety of intra- and extra-cellular antimicrobial functions. In the present study we show, for the first time, that the transcriptional regulator HIF-1α (hypoxia-inducible factor-1α) mediates the extracellular antimicrobial activity of human and murine MCs by increasing the formation of MCETs (MC extracellular traps).


Cosmic Ray Particles Images With Orca-Ii Erg, George Mcnamara Aug 2012

Cosmic Ray Particles Images With Orca-Ii Erg, George Mcnamara

George McNamara

Cosmic ray particles image series acquired using a Hamamatsu ORCA-II ERG scientific grade CCD camera, cooled to -60 C. Each image is a consecutive 600 second (10 minute) exposure time with no light to the camera.

While processing the data, I discoverd that the background changed around planes 25 and 227 (see Excel file and jpeg screenshots), so I also processed only planes 025-227 (203 planes total, 2030 minutes, 33.83 hours). the CCD industry "rule of thumb" for a "typical" CCD sensor (i.e. 1/3" CCD) is that one cosmic ray particle strikes a sensor approximately every 30 ...


Trace Elements Affect Methanogenic Activity And Diversity In Enrichments From Subsurface Coal Bed Produced Water, B. Unal, V. R. Perry, M. Sheth, V. Gomez-Alvarez, K. J. Chin, Klaus R. Nusslein May 2012

Trace Elements Affect Methanogenic Activity And Diversity In Enrichments From Subsurface Coal Bed Produced Water, B. Unal, V. R. Perry, M. Sheth, V. Gomez-Alvarez, K. J. Chin, Klaus R. Nusslein

Klaus R. Nusslein

Microbial methane from coal beds accounts for a significant and growing percentage of natural gas worldwide. Our knowledge of physical and geochemical factors regulating methanogenesis is still in its infancy. We hypothesized that in these closed systems, trace elements (as micronutrients) are a limiting factor for methanogenic growth and activity. Trace elements are essential components of enzymes or cofactors of metabolic pathways associated with methanogenesis. This study examined the effects of eight trace elements (iron, nickel, cobalt, molybdenum, zinc, manganese, boron, and copper) on methane production, on mcrA transcript levels, and on methanogenic community structure in enrichment cultures obtained from ...


Clay Fabric And Mass Physical Properties Of Surficial Marine Sediment Near The Deepwater Horizon Oil Spill, Andrew Head, Richard H. Bennett, Jessica R. Douglas, Kenneth J. Curry Feb 2012

Clay Fabric And Mass Physical Properties Of Surficial Marine Sediment Near The Deepwater Horizon Oil Spill, Andrew Head, Richard H. Bennett, Jessica R. Douglas, Kenneth J. Curry

Kenneth J. Curry

Surficial sediment was obtained on the RV Cape Hatteras Cruise (2010) from the seafloor at a water depth of 1570 meters located at latitude 28°44'20.16"N and longitude 88°20'24.96"W in close proximity to the Deepwater Horizon well, Gulf of Mexico. Preliminary clay nano- and microfabric observation using a transmission electron microscope (TEM) depicted a sediment rich in clays and organic matter (OM) especially in the upper 2 cm subbottom. Initial analysis of TEM micrographs depicted a high porosity clay sediment. Initial study of the mass physical properties revealed water content ωt = 67.32 ...


Pubspectra - Open Data Access Fluorescence Spectra, George Mcnamara Feb 2012

Pubspectra - Open Data Access Fluorescence Spectra, George Mcnamara

George McNamara

The Internet is enabling greater access to spectral imaging publications, spectral graphs, and data than that was available a generation ago. The spectral imaging systems discussed in this issue of Cytometry work because reagent and hardware spectra are reproducible, reusable, and provide input to spectral unmixing and spectral components recognition algorithms. These spectra need to be readily available in order to determine what to purchase, how to use it, and what the output means. We refer to several commercially sponsored and academic spectral web sites and discuss our spectral graphing and data sites. Sites include fluorescent dye graph servers from ...


Mcnamara 2011 Feature Extraction (Image Analysis), George Mcnamara Feb 2012

Mcnamara 2011 Feature Extraction (Image Analysis), George Mcnamara

George McNamara

Feature Extraction presentation and movies in a ZIP file from a presentation I gave at ISAC 2011 in Baltomore, Md.

Feature extraction is one phrase for image analysis.


Conformations Of The Apo-, Substrate-Bound And Phosphate-Bound Atp-Binding Domain Of The Cu(Ii) Atpase Copb Illustrate Coupling Of Domain Movement To The Catalytic Cycle, José Argüello, Samuel Jayakanthan, Sue Roberts, Andrzej Weichsel, Megan Mcevoy Dec 2011

Conformations Of The Apo-, Substrate-Bound And Phosphate-Bound Atp-Binding Domain Of The Cu(Ii) Atpase Copb Illustrate Coupling Of Domain Movement To The Catalytic Cycle, José Argüello, Samuel Jayakanthan, Sue Roberts, Andrzej Weichsel, Megan Mcevoy

José M. Argüello

Heavy metal P1B-type ATPases play a critical role in cell survival by maintaining appropriate intracellular metal concentrations. Archaeoglobus fulgidus CopB is a member of this family that transports Cu(II) from the cytoplasm to the exterior of the cell using ATP as energy source. CopB has a 264 amino acid ATPBD (ATP-binding domain) that is essential for ATP binding and hydrolysis as well as ultimately transducing the energy to the transmembrane metal-binding site for metal occlusion and export. The relevant conformations of this domain during the different steps of the catalytic cycle are still under discussion. Through crystal structures of ...


Pharmacological Inhibition Of The Clpxp Protease Increases Bacterial Susceptibility To Host Cathelicidin Antimicrobial Peptides And Cell-Envelope Active Antibiotics., S. Mcgillivray, D. Tran, N. Ramadoss, J. Alumasa, Cheryl Okumura, G. Sakoulas, M. Vaughn, D. Zhang, K. Keiler, V. Nizet Dec 2011

Pharmacological Inhibition Of The Clpxp Protease Increases Bacterial Susceptibility To Host Cathelicidin Antimicrobial Peptides And Cell-Envelope Active Antibiotics., S. Mcgillivray, D. Tran, N. Ramadoss, J. Alumasa, Cheryl Okumura, G. Sakoulas, M. Vaughn, D. Zhang, K. Keiler, V. Nizet

Cheryl Okumura

The ClpXP protease is a critical bacterial intracellular protease that regulates protein turnover in many bacterial species. Here we identified a pharmacological inhibitor of the ClpXP protease, F2, and evaluated its action in Bacillus anthracis and Staphylococcus aureus. We found that F2 exhibited synergistic antimicrobial activity with cathelicidin antimicrobial peptides and antibiotics that target the cell well and/or cell membrane such as penicillin and daptomycin in B. anthracis and drug resistant strains of S. aureus. ClpXP inhibition represents a novel therapeutic strategy to simultaneously sensitize pathogenic bacteria to host defenses and pharmaceutical antibiotics.