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2008

Microfilament Proteins

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Ponticulin Is An Atypical Membrane Protein, Anne L. Hitt, Tze Hong Lu, Elizabeth J. Luna Mar 2008

Ponticulin Is An Atypical Membrane Protein, Anne L. Hitt, Tze Hong Lu, Elizabeth J. Luna

Elizabeth J. Luna

We have cloned and sequenced ponticulin, a 17,000-dalton integral membrane glycoprotein that binds F-actin and nucleates actin assembly. A single copy gene encodes a developmentally regulated message that is high during growth and early development, but drops precipitously during cell streaming at approximately 8 h of development. The deduced amino acid sequence predicts a protein with a cleaved NH2-terminal signal sequence and a COOH-terminal glycosyl anchor. These predictions are supported by amino acid sequencing of mature ponticulin and metabolic labeling with glycosyl anchor components. Although no alpha-helical membrane-spanning domains are apparent, several hydrophobic and/or sided beta-strands, each long ...