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Full-Text Articles in Life Sciences

Network Analyses Of Glomerular Capillaries, Jason Cory Brunson, Justin Sardi, Mark Terasaki May 2019

Network Analyses Of Glomerular Capillaries, Jason Cory Brunson, Justin Sardi, Mark Terasaki

Biology and Medicine Through Mathematics Conference

No abstract provided.


Β Cell Network Dysfunction In Pancreatic Islets By Silencing Hub Cells, Janita Patwardhan, Bradford E. Peercy May 2019

Β Cell Network Dysfunction In Pancreatic Islets By Silencing Hub Cells, Janita Patwardhan, Bradford E. Peercy

Biology and Medicine Through Mathematics Conference

No abstract provided.


Real-Time Interactive Simulation Of Human Atrial Electrical Activity On Realistic 3d Atrial Structures Using Webgl, Abouzar Kaboudian, Yanyan Ji, Elizabeth Cherry, Flavio H. Fenton May 2019

Real-Time Interactive Simulation Of Human Atrial Electrical Activity On Realistic 3d Atrial Structures Using Webgl, Abouzar Kaboudian, Yanyan Ji, Elizabeth Cherry, Flavio H. Fenton

Biology and Medicine Through Mathematics Conference

No abstract provided.


Live (Boxing) Liam Smith Vs Sam Eggington Live Stream Ppv Fight, Smith Eggington Mar 2019

Live (Boxing) Liam Smith Vs Sam Eggington Live Stream Ppv Fight, Smith Eggington

Smith Eggington

Liam Smith fights in his home city of Liverpool for the first time in three years on Saturday as he takes on Sam Eggington.




The former WBO super-welterweight champion is back on the title hunt having linked up with Eddie Hearn and Matchroom Boxing.

The Liverpudlian fell just short fighting for that belt against Jaime Munguia in Las Vegas last summer, losing on an unanimous decision in what was just the second defeat of his career - the first coming against ...


Data Collection Curated With An Application Ontology Describes The Methods And Results Upon Performing An Ex-Vivo Voltage-Clamp Assay On Outer Hair Cells Of The Mammalian Cochlea, Brenda Farrell, Jason Bengtson Jan 2019

Data Collection Curated With An Application Ontology Describes The Methods And Results Upon Performing An Ex-Vivo Voltage-Clamp Assay On Outer Hair Cells Of The Mammalian Cochlea, Brenda Farrell, Jason Bengtson

BCM Research Data

This data collection describes the electrical properties of outer hair cells isolated from the mammalian cochlea of the domestic guinea pig. This data was obtained by performing whole-cell patch clamp voltage clamp assay on cells and monitoring the electrical admittance during a DC voltage ramp. The membrane capacitance was then calculated at each membrane potential from this admittance, and the voltage-independent and voltage-dependent membrane capacitance was determined upon further analysis. In some case the DC conductance was also measured by interrogation of the cell with voltage-step function which was calculated from the change in the mean steady-state current with respect ...


Polygenic Scores For Metabolic Traits And Related Drug Prescriptions In The Michigan Genomics Initiative, Samuel K. Handelman, Xiaomeng Du, Yanhua H. Chen, Elizabeth K. Speliotes Jun 2018

Polygenic Scores For Metabolic Traits And Related Drug Prescriptions In The Michigan Genomics Initiative, Samuel K. Handelman, Xiaomeng Du, Yanhua H. Chen, Elizabeth K. Speliotes

Biology and Medicine Through Mathematics Conference

No abstract provided.


Modeling The Evolutionary History Of A Tumor, Julia Chifman May 2018

Modeling The Evolutionary History Of A Tumor, Julia Chifman

Biology and Medicine Through Mathematics Conference

No abstract provided.


A Time Course Study Of Rotavirus-Infected Intestinal Cells Treated With Stilbenoids And The Regulation Of Apoptosis, Rebecca D. Parr, Caleb M. Witcher, Rebekah Napier-Jameson, Hannah L. Wilson, Stormey Wisdom, Macie N. Mattila, Essence B. Strange, Josephine Taylor, Beatrice Clack, Fabricio Medina-Bolivar, Judith M. Ball May 2018

A Time Course Study Of Rotavirus-Infected Intestinal Cells Treated With Stilbenoids And The Regulation Of Apoptosis, Rebecca D. Parr, Caleb M. Witcher, Rebekah Napier-Jameson, Hannah L. Wilson, Stormey Wisdom, Macie N. Mattila, Essence B. Strange, Josephine Taylor, Beatrice Clack, Fabricio Medina-Bolivar, Judith M. Ball

Faculty Publications

This is a time course study of virus –host interactions that are modified with the addition of two small natural products. They appear to effect virus replication and the host response to the infection.


Frozen Brain Atlas, Natalie Andrea Builes, Dominick Joseph Casciato, Samantha Taylor Bergoine, Robert Speth Jul 2017

Frozen Brain Atlas, Natalie Andrea Builes, Dominick Joseph Casciato, Samantha Taylor Bergoine, Robert Speth

Robert Speth

The Frozen Brain Atlas provides students, educators, and scientists with a tool to further explore the architecture of the brain. Current brain atlases allow users to navigate sectioned brain tissue using histological staining; however, they fail to capture naturally visible structures post sectioning. Through immediate post sectioning image collection, the natural contrast of the brain reveals unique structural details that are rarely seen by traditional staining procedures. By utilizing indexing and interactive labeling, the Nova Southeastern University Frozen Brain Atlas will allow identification of brain regions when used as a correlative tool for modalities including receptor autoradiography, immunofluorescence, and other ...


Repeated Exposure To Methamphetamine Induces Sex-Dependent Hypersensitivity To Ischemic Injury In The Adult Rat Heart, Boyd R. Rorabaugh, Sarah L. Seeley, Thorne S. Stoops, Manoranjan S. D’Souza Jun 2017

Repeated Exposure To Methamphetamine Induces Sex-Dependent Hypersensitivity To Ischemic Injury In The Adult Rat Heart, Boyd R. Rorabaugh, Sarah L. Seeley, Thorne S. Stoops, Manoranjan S. D’Souza

Pharmacy Faculty Scholarship

BACKGROUND: We previously reported that adult female, but not male rats that were prenatally exposed to methamphetamine exhibit myocardial hypersensitivity to ischemic injury. However, it is unknown whether hypersensitivity to ischemic injury develops when rats are exposed to methamphetamine during adulthood. The goal of this study was to determine whether methamphetamine exposure during adulthood sensitizes the heart to ischemic injury. METHODS: Adult male and female rats received daily injections of methamphetamine (5 mg/kg) or saline for 10 days. Their hearts were isolated on day 11 and subjected to a 20 min ischemic insult on a Langendorff isolated heart apparatus ...


High Performance Computation Of Cardiac Models In Real-Time Using Webgl, Abouzar Kaboudian, Flavio H. Fenton May 2017

High Performance Computation Of Cardiac Models In Real-Time Using Webgl, Abouzar Kaboudian, Flavio H. Fenton

Biology and Medicine Through Mathematics Conference

No abstract provided.


Experimental Design For Parameter Estimation In An Allometric Food Web Model, Amanda Laubmeier May 2017

Experimental Design For Parameter Estimation In An Allometric Food Web Model, Amanda Laubmeier

Biology and Medicine Through Mathematics Conference

No abstract provided.


Modeling The Breakup Of A Two-Layer Tear Film, Michael Stapf May 2017

Modeling The Breakup Of A Two-Layer Tear Film, Michael Stapf

Biology and Medicine Through Mathematics Conference

No abstract provided.


Susceptibility Of Chlamydia Trachomatis To The Excipient Hydroxy-Ethylcellulose: Ph And Concentration Dependence Of Antimicrobial Activity, Ali A. Abdul-Sater, David M. Ojcius, M. P. Meyer Apr 2017

Susceptibility Of Chlamydia Trachomatis To The Excipient Hydroxy-Ethylcellulose: Ph And Concentration Dependence Of Antimicrobial Activity, Ali A. Abdul-Sater, David M. Ojcius, M. P. Meyer

David M. Ojcius

Hydroxyethyl cellulose (HEC) is used as a neutral excipient in microbicides used against sexually transmitted pathogens. However, HEC inhibits the infection of cervical epithelial cells by Chlamydia trachomatis at pH 5 in a concentration-dependent manner. At pH 7, infection is inversely dependent on the concentration of HEC, possibly due to pH-dependent calcium sequestration.


Silencing Science.Pptx, Kai Hung Jan 2017

Silencing Science.Pptx, Kai Hung

Faculty Research & Creative Activity

This is a non-peer reviewed teaching resource.


Obat Kutil Kelamin Herbal Denature Di Denpasar Untuk Pria Dan Wanita, Obat Kutil Kelamin Denature Yang Alami Dec 2016

Obat Kutil Kelamin Herbal Denature Di Denpasar Untuk Pria Dan Wanita, Obat Kutil Kelamin Denature Yang Alami

Obat Herbal Pria Wanita De Nature

Kutil kelamin biasanya terjadi dalam kelompok, bisa berukuran kecil atau besar dan tersebar di bagian kutil kelamin, penis atau sekitar anus yang menimbulkan gatal pada pria dan wanita. Jika yang menjadi penderita kutil kelamin adalah wanita maka biasanya akan mempengaruhi bagian luar vagina, di dalam vagina, di rahim, atau pembukaan serviks.


Effect Of Time Duration Of Digestion/Decontamination Technique On Yield Of Mycobacteria And Contamination Rates From Sterile Body Fluids (Pleural And Ascitic Fluid) And Pus Specimens, Samreen Shafiq, Faryal Saleem, Kauser Jabeen, Joveria Farooqi, Warda Alam, Sadia Hanif, Shazia Ali, Sadia Shakoor, Rumina Hasan Dec 2016

Effect Of Time Duration Of Digestion/Decontamination Technique On Yield Of Mycobacteria And Contamination Rates From Sterile Body Fluids (Pleural And Ascitic Fluid) And Pus Specimens, Samreen Shafiq, Faryal Saleem, Kauser Jabeen, Joveria Farooqi, Warda Alam, Sadia Hanif, Shazia Ali, Sadia Shakoor, Rumina Hasan

Department of Pathology and Laboratory Medicine

Background:
Duration of digestion/decontamination has a considerable impact on yield of mycobacteria especially from sterile body fluids and pus specimens. Additionally, duration of digestion/decontamination affects the contamination rates. This study evaluates the effect of digestion/decontamination protocol for 15 and 20min versus inoculation of media directly from the sample on contamination rates as well as the yield of mycobacteria from pus and sterile fluids other than cerebrospinal fluids.
Methods:
Pleural fluid (n=60), pus (n=48) and ascitic fluid (n=12) specimens were cultured for mycobacteria and evaluated for contamination and mycobacterial yield using three different processing methodologies ...


A Population-Based Study To Investigate Host Genetic Factors Associated With Hepatitis B Infection And Pathogenesis In The Chinese Population, Zheng Zheng, Li Guan, Ping An, Shan Sun, Stephen J. O'Brien, Cheryl Winkler Oct 2016

A Population-Based Study To Investigate Host Genetic Factors Associated With Hepatitis B Infection And Pathogenesis In The Chinese Population, Zheng Zheng, Li Guan, Ping An, Shan Sun, Stephen J. O'Brien, Cheryl Winkler

Stephen O'Brien

Background Hepatitis B virus (HBV) infection is a significant public health problem that may lead to chronic liver disease, cirrhosis, and hepatocellular carcinoma (HCC). Approximately 30% of the world's population has been infected with HBV and approximately 350 million (5–6%) are persistent carriers. More than 120 million Chinese are infected with HBV. The role of host genetic factors and their interactions with environmental factors leading to chronic HBV infection and its complications are not well understood. We believe that a better understanding of these factors and interactions will lead to more effective diagnostic and therapeutic options. Methods/Design ...


Frozen Brain Atlas, Natalie Andrea Builes, Dominick Joseph Casciato, Samantha Taylor Bergoine, Robert Speth Sep 2016

Frozen Brain Atlas, Natalie Andrea Builes, Dominick Joseph Casciato, Samantha Taylor Bergoine, Robert Speth

Frozen Brain Atlas

The Frozen Brain Atlas provides students, educators, and scientists with a tool to further explore the architecture of the brain. Current brain atlases allow users to navigate sectioned brain tissue using histological staining; however, they fail to capture naturally visible structures post sectioning. Through immediate post sectioning image collection, the natural contrast of the brain reveals unique structural details that are rarely seen by traditional staining procedures. By utilizing indexing and interactive labeling, the Nova Southeastern University Frozen Brain Atlas will allow identification of brain regions when used as a correlative tool for modalities including receptor autoradiography, immunofluorescence, and other ...


Prenatal Methamphetamine Differentially Alters Myocardial Sensitivity To Ischemic Injury In Male And Female Adult Rats, Boyd R. Rorabaugh, Sarah L. Seeley, Albert D. Bui, Lisanne Sprague, Manoranjan S. D’Souza Feb 2016

Prenatal Methamphetamine Differentially Alters Myocardial Sensitivity To Ischemic Injury In Male And Female Adult Rats, Boyd R. Rorabaugh, Sarah L. Seeley, Albert D. Bui, Lisanne Sprague, Manoranjan S. D’Souza

Pharmacy Faculty Scholarship

Methamphetamine is one of the most common illicit drugs abused during pregnancy. The neurological effects of prenatal methamphetamine are well known. However, few studies have investigated the potential effects of prenatal methamphetamine on adult cardiovascular function. Previous work demonstrated that prenatal cocaine exposure increases sensitivity of the adult heart to ischemic injury. Methamphetamine and cocaine have different mechanisms of action, but both drugs exert their effects by increasing dopaminergic and adrenergic receptor stimulation. Thus the goal of this study was to determine whether prenatal methamphetamine also worsens ischemic injury in the adult heart. Pregnant rats were injected with methamphetamine (5 ...


Movement Path Tortuosity In Free Ambulation: Relationships To Age And Brain Disease, William Kearns, James Fozard, Vilis Nams Jan 2016

Movement Path Tortuosity In Free Ambulation: Relationships To Age And Brain Disease, William Kearns, James Fozard, Vilis Nams

William D. Kearns, PhD

Ambulation is defined by duration, distance traversed, number and size of directional changes and the interval separating successive movement episodes; more complex measures of ambulation can be created by aggregating these features. This review article of published findings defines random changes in direction during movement as “movement path tortuosity”, and relates tortuosity to the understanding of cognitive impairments of persons of all ages. Path tortuosity is quantified by subjecting tracking data to fractal analysis, specifically Fractal Dimension (Fractal D), which ranges from a value of 1 when the movement path is perfectly straight to a value of 2 when the ...


Human Anatomy And Physiology Preparatory Course: Part 1 Of 4 (Interactive), Carlos Liachovitzky Jan 2016

Human Anatomy And Physiology Preparatory Course: Part 1 Of 4 (Interactive), Carlos Liachovitzky

Open Educational Resources

The overall purpose of these preparatory course set of learning objectives is to help students familiarize with some terms and some basic concepts they will find later in the Human Anatomy and Physiology I course.

These 40+ learning objectives to prepare for Human Anatomy and Physiology can be downloaded and played in a desktop, or laptop (windows exe file).

The entire course has four parts:

Each learning objective is followed by a set of multiple choice question similar to those found later in a Human Anatomy and Physiology course.

The organization and ...


Synaptogyrin 3 Binds Amyloid Beta And May Alter Dopamine Transport In Alzheimer’S Disease, William Gierach Jan 2016

Synaptogyrin 3 Binds Amyloid Beta And May Alter Dopamine Transport In Alzheimer’S Disease, William Gierach

Undergraduate Honors Theses

Alzheimer’s Disease effects 35 million elderly individuals globally by causing dementia, memory loss and eventually death. Amyloid Beta (Aß), a neurotropic peptide and a hallmark of AD pathogenesis, disrupts cellular organization and impairs neuronal systems. The dopaminergic system regulates learning, motor function and behavior and malfunctions in early AD. Aß causes dopaminergic transmission to dysfunction by unknown mechanisms. To elucidate these mechanisms, we screened solubilized mouse synaptosomes for proteins that specifically bind Aß 1-42. 107 proteins that bound Aß were identified by mass spectrometry. Human orthologs were further screened in HEK293 cells for Aß binding. Of the thirteen proteins ...


Focus On Microscopy Fom 2006-2015 Abstracts, George Mcnamara Apr 2015

Focus On Microscopy Fom 2006-2015 Abstracts, George Mcnamara

George McNamara

Focus On Microscopy FOM 2006-2015 abstracts

FOM's are cutting edge microscopy meetings. Their abstracts are posted online soon after the meeting. FOM2015's abstracts are at http://www.focusonmicroscopy.org/2015/program.html

I have been downloading the single PDF abstracts, and making a single large PDF. every year. I figure the "annual organized" PDF may be useful to others, so am posting a ZIP file here containing: FOM 2006 FOM 2007 FOM 2008 FOM 2009 FOM 2010 FOM 2011 FOM 2012 FOM 2013 FOM 2014 FOM 2015 I note the abstracts do not contain copyright notices, but anyone ...


Pathscan Enabler At Md Anderson Cancer Center, George Mcnamara Jul 2014

Pathscan Enabler At Md Anderson Cancer Center, George Mcnamara

George McNamara

McNamara 20140703 - Additional Pathscan and Tiki_Goddess related resources

http://0-works.bepress.com.library.simmons.edu/gmcnamara/52

http://home.earthlink.net/~tiki_goddess/

http://0-works.bepress.com.library.simmons.edu/gmcnamara/1/

http://0-works.bepress.com.library.simmons.edu/gmcnamara/11/

https://www.linkedin.com/in/georgemcnamara

Our Pathscan Enabler IV, delivered July 2014, uses the QuickScan software to scan a histology slide in "two clicks" (after loading the

slide):

1. Push the QuickScan button on the front of the Pathscan Enabler.

2. Click the Scan button in the QuickScan pop-up application (optional: change scan area in the Prescan image window).

The image gets saved as a TIFF file to ...


Dengue Virus-Specific Human Cd4+ T-Lymphocyte Responses In A Recipient Of An Experimental Live-Attenuated Dengue Virus Type 1 Vaccine: Bulk Culture Proliferation, Clonal Analysis, And Precursor Frequency Determination, Sharone Green, Ichiro Kurane, Robert Edelman, Carol 0. Tacket, Kenneth H. Eckels, David W. Vaughn, Charles H. Hoke, Francis A. Ennis Jan 2014

Dengue Virus-Specific Human Cd4+ T-Lymphocyte Responses In A Recipient Of An Experimental Live-Attenuated Dengue Virus Type 1 Vaccine: Bulk Culture Proliferation, Clonal Analysis, And Precursor Frequency Determination, Sharone Green, Ichiro Kurane, Robert Edelman, Carol 0. Tacket, Kenneth H. Eckels, David W. Vaughn, Charles H. Hoke, Francis A. Ennis

Sharone Green

We analyzed the CD4+ T-lymphocyte responses to dengue, West Nile, and yellow fever viruses 4 months after immunization of a volunteer with an experimental live-attenuated dengue virus type 1 vaccine (DEN-1 45AZ5). We examined bulk culture proliferation to noninfectious antigens, determined the precursor frequency of specific CD4+ T cells by limiting dilution, and established and analyzed CD4+ T-cell clones. Bulk culture proliferation was predominantly dengue virus type 1 specific with a lesser degree of cross-reactive responses to other dengue virus serotypes, West Nile virus, and yellow fever virus. Precursor frequency determination by limiting dilution in the presence of noninfectious dengue ...


Leica Microscope Gpu Deconvolution Stellaris Fish Dataset #1, George Mcnamara Nov 2013

Leica Microscope Gpu Deconvolution Stellaris Fish Dataset #1, George Mcnamara

George McNamara

McNamara 20131101Fri Leica widefield microscope CUDA Deconvolution Stellaris FISH probe cultured cells dataset #1.zip

A text file in the zip archive has experiment details. I am posting this online so that researchers - whether academic or commercial - can evaluate the acquired data, the Bruce and Butte 2013 Optics Express ( http://www.opticsinfobase.org/oe/fulltext.cfm?uri=oe-21-4-4766 ) deconvolution result (note: I may not have used optimal settings), and to compare these deconvolution results to other methods. If anyone generates alternative spatial deconvolution output, such as from: * SVI Huygens * Media Cy AutoQuant * Agard's ER-Decon (Arigovindan 2013 PNAS) * Vicidomini SGP ...


Responding To Farm-Related Injuries, Charles V. Schwab Apr 2013

Responding To Farm-Related Injuries, Charles V. Schwab

Charles V. Schwab

No abstract provided.


Flash4 Dark Reference Images, George Mcnamara Apr 2013

Flash4 Dark Reference Images, George Mcnamara

George McNamara

Hamamatsu FLASH4.0 dark reference images, acquired with 10 second exposure times, no light to camera. Camera offset (set by Hamamatsu( is ~100 (the average intensity of the first image is always ~1 intensity level higher - an odd feature, but trivial in practice for a 16-bit camera).

George McNamara, Ph.D.

Single Cells Analyst at L.J.N. Cooper Lab

University of Texas M.D. Anderson Cancer Center


Pubspectra Tattletales, George Mcnamara Feb 2013

Pubspectra Tattletales, George Mcnamara

George McNamara

Tattletales for Multiplex Fluorescent Reporters in Single Cells for Metabolomics

George McNamara

As of April 2013: L.J.N. Cooper & D.A. Lee Cellular Immunotherapy Lab, University of Texas M.D. Anderson Cancer Center, Houston, TX

Email: gtmcnamara@mdanderson.org, geomcnamara@earthlink.net

Tattletales is my concept for spatial multiplexing many fluorescent protein (FP) biosensors in the same live cell. For example, there are excellent FP biosensors to Ca++ ions, pH, glucose, ribose, glutamine, glutamate, ATP, redox, ROS, pyruvate, cAMP, cGMP, IP3, PI(3,4,5)P3, cell cycle indicators (Fucci2), PKA, PKC, photsphatases, caspase(s) [1, 2]. However, these are typically used one biosensor per experiment, due in part to flooding the cell with soluble biosensor. That is, conventionally, either a metabolite (glucose) reporter or a signal transduction (Ca++) reporter can be imaged. By flooding the cell with the reporter, signal to noise ratio is compromized by autofluorescence.

Tattletales takes advantage of spatial multiplexing to both increase the number of different reporters, and improve signal to noise ratio by localizing each biosensor to a small volume. I started with the observation by Robinett et al [3] who localized 512 GFP-nls-LacI to a 256 LacO array as a 200 nm diameter diffraction limited spot (nuclear background due to overexpression). Many thousands of DNA binding proteins, of known sequence specifities, exist (LacI, TetR, GalR, etc for cell line studies; ZF-FPs, TALE-FPs to STRs, telomere repeat binding factors-FPs, etc for primary cells) and can be fused (as cDNAs) to different fluorescent proteins and FP biosensors.

Many biosensors are available as affinity series [1, 4], now enabling extended dynamic range. I realized that spatial multiplexing of many DNA binding protein-reporters by localizing to different spots in the cell nucleus and distinguished by combinatorial addressing, where N address colors provide 2^N addresses (example, 3 colors is 2^3 = 8 combinations). Multiplexing ...