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Full-Text Articles in Life Sciences

Onecut1 And Onecut2 Play Critical Roles In The Development Of The Mouse Retina, Jillian J. Goetz, Gregory M. Martin, Rebecca Chowdhury, Jeffrey M. Trimarchi Jan 2014

Onecut1 And Onecut2 Play Critical Roles In The Development Of The Mouse Retina, Jillian J. Goetz, Gregory M. Martin, Rebecca Chowdhury, Jeffrey M. Trimarchi

Genetics, Development and Cell Biology Publications

The entire repertoire of intrinsic factors that control the cell fate determination process of specific retinal neurons has yet to be fully identified. Single cell transcriptome profiling experiments of retinal progenitor cells revealed considerable gene expression heterogeneity between individual cells, especially among different classes of transcription factors. In this study, we show that two of those factors, Onecut1 and Onecut2, are expressed during mouse retinal development. Using mice that are deficient for each of these transcription factors, we further demonstrate a significant loss (∼70–80%) of horizontal cells in the absence of either of these proteins, while the other retinal ...


Computational Modeling Suggests Dimerization Of Equine Infectious Anemia Virus Rev Is Required For Rna Binding, Chijioke N. Umunnakwe, Hyelee Loyd, Kinsey Cornick, Jerald R. Chavez, Drena Dobbs, Susan Carpenter Jan 2014

Computational Modeling Suggests Dimerization Of Equine Infectious Anemia Virus Rev Is Required For Rna Binding, Chijioke N. Umunnakwe, Hyelee Loyd, Kinsey Cornick, Jerald R. Chavez, Drena Dobbs, Susan Carpenter

Genetics, Development and Cell Biology Publications

Background

The lentiviral Rev protein mediates nuclear export of intron-containing viral RNAs that encode structural proteins or serve as the viral genome. Following translation, HIV-1 Rev localizes to the nucleus and binds its cognate sequence, termed the Rev-responsive element (RRE), in incompletely spliced viral RNA. Rev subsequently multimerizes along the viral RNA and associates with the cellular Crm1 export machinery to translocate the RNA-protein complex to the cytoplasm. Equine infectious anemia virus (EIAV) Rev is functionally homologous to HIV-1 Rev, but shares very little sequence similarity and differs in domain organization. EIAV Rev also contains a bipartite RNA binding domain ...


Loss Of Gq/11 Genes Does Not Abolish Melanopsin Phototransduction, Kylie S. Chew, Tiffany M. Schmidt, Alan C. Rupp, Paulo Kofuji, Jeffrey M. Trimarchi Jan 2014

Loss Of Gq/11 Genes Does Not Abolish Melanopsin Phototransduction, Kylie S. Chew, Tiffany M. Schmidt, Alan C. Rupp, Paulo Kofuji, Jeffrey M. Trimarchi

Genetics, Development and Cell Biology Publications

In mammals, a subset of retinal ganglion cells (RGCs) expresses the photopigment melanopsin, which renders them intrinsically photosensitive (ipRGCs). These ipRGCs mediate various non-image-forming visual functions such as circadian photoentrainment and the pupillary light reflex (PLR). Melanopsin phototransduction begins with activation of a heterotrimeric G protein of unknown identity. Several studies of melanopsin phototransduction have implicated a G-protein of the Gq/11 family, which consists of Gna11, Gna14, Gnaq and Gna15, in melanopsin-evoked depolarization. However, the exact identity of the Gq/11 gene involved in this process has remained elusive. Additionally, whether Gq/11 G-proteins are necessary for melanopsin phototransduction ...