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Full-Text Articles in Life Sciences

Analysis Of Antimicrobial Peptide Efficacy Against Chytridiomycosis From Skin Secretions Of Columbia Spotted Frogs (Lithobates Luteiventris), Emma Esposito Apr 2018

Analysis Of Antimicrobial Peptide Efficacy Against Chytridiomycosis From Skin Secretions Of Columbia Spotted Frogs (Lithobates Luteiventris), Emma Esposito

Life and Environmental Sciences Undergraduate Theses

Amphibian populations have been declining in size in recent years. A major contributing factor to this decline is the fungal disease chytridiomycosis. Chytridiomycosis occurs when the zoospores of the fungus Batrachochytrium dendrobatidis (Bd) imbed into amphibian skin and disrupt the homeostatic functions the skin provides, leading to death in most amphibians. Amphibian skin can produce antimicrobial peptides (AMPs) that inhibit Bd infection. The goal of this experiment was to determine whether or not the amount and type of AMPs a frog produces affect its ability to defend against chytridiomycosis. Columbia spotted frogs were chosen as a model organism because they ...


Amphibian Risk Assessment In Montana: An Evaluation Of Chytridiomycosis In Columbia Spotted Frogs (Lithobates Luteiventris), Alex Kurtz May 2017

Amphibian Risk Assessment In Montana: An Evaluation Of Chytridiomycosis In Columbia Spotted Frogs (Lithobates Luteiventris), Alex Kurtz

Life and Environmental Sciences Undergraduate Theses

The fungus, Batrachochytrium dendrobatidis, has been found to negatively impact amphibian populations around the world. This fungus can have multiple effects on frog physiology, including changes in osmotic regulation that may lead to death. B. dendrobatidis has been shown to be the driving force for many amphibian population crashes and extinctions around the world. The purpose of this project was to build a predictive model of B. dendrobatidis infection, one that would be used to assess population susceptibility in order to identify populations of amphibians at risk of infection. This was accomplished by statistical analyses of several components that contribute ...


Using Glycosylated Hemoglobin And Heat Shock Protein 70 As Thermal Biomarkers In North American Pikas (Ochotona Princeps), Steven Edmonds Apr 2016

Using Glycosylated Hemoglobin And Heat Shock Protein 70 As Thermal Biomarkers In North American Pikas (Ochotona Princeps), Steven Edmonds

Life and Environmental Sciences Undergraduate Theses

Pikas (Ochotona princeps), high altitude lagomorphs, are potentially one of the first mammals to be directly affected by global warming. Population decline has been observed in pika populations in Nevada and California. The cause of their decline is unknown but several studies suggest that heat stress, especially at lower altitudes, is a contributing factor. In Montana, populations are potentially stressed in the same way. One hypothesis is that direct thermal stress is causing population decline. This study looks at heat shock protein 70 (HSP70) and glycosylated hemoglobin levels in high and low altitude pika populations as biomarkers for thermal stress ...


Detection Of Inducible Hsp70 As A Measure Of Heat Stress In Mammalian Tissue, Stuart Allyn Apr 2014

Detection Of Inducible Hsp70 As A Measure Of Heat Stress In Mammalian Tissue, Stuart Allyn

Life and Environmental Sciences Undergraduate Theses

The North American Pika, Ochotona princeps, is a keystone high altitude species whose populations have been declining and whose distributions have been receding to higher elevations in the Great Basin. The present study proposes that these population shifts may be occurring because O. princeps are thermally stressed, as indicated by high levels of the inducible form of Heat Shock Protein 70 (HSP70). Ochotona princeps blood samples were collected from a range of elevations at different temperatures. Western Blot analysis was used as a qualitative measure to detect the presence of HSP70 in various mammalian and cell line samples to prepare ...


Molecular Genetic Affinities Of The Melanistic Western Terrestrial Garter Snake, Thamnophis Elegans, Kevyn Stroebe Apr 2009

Molecular Genetic Affinities Of The Melanistic Western Terrestrial Garter Snake, Thamnophis Elegans, Kevyn Stroebe

Life and Environmental Sciences Undergraduate Theses

Polymorphic pigmentation in natural populations has served as a model for understanding diversity and evolutionary change among populations. Melanism is a rare phenotype in garter snake populations that is used to study the adaptation of color and evolutionary processes associated with change in pigmentation among populations. Additionally, melanistic phenotypes can complicate species identification in situations of conservation interest. This study examined the association between phenotype and species of garter snakes found along the Missouri River near Townsend, MT by analyzing Cytochrome b mitochondrial DNA sequences of Thamnophis elegans (Western Terrestrial Garter Snake), T. sirtalis (Common Garter Snake), and melanistic individuals ...


The Internal Hoof Morphology Of The Pl, P2, And P3 Bones In Equine, Heather Caldwell Apr 2009

The Internal Hoof Morphology Of The Pl, P2, And P3 Bones In Equine, Heather Caldwell

Life and Environmental Sciences Undergraduate Theses

The purpose of this study was to investigate the P3 pedal angle of the feet of healthy equine exhibiting no P3 bone rotation in comparison to equine with corrective shoeing or apparent P3 bone rotation or laminitis. The internal pedal bones of 51 equine were radiographed, digitally photographed, and scanned into the EponaTech program. The overall internal pedal anatomy of the Pl, P2, P3, and navicular bone was compared between all 51 equine. Seven landmarks were used in assessment: angle of the palmer surface of P3, hoof wall to coffin bone length, dorsal hoof wall depth, length of the bottom ...


Efficiency And Accuracy Of Geometric Morphometries In The Analysis Of Intraspecific Variation In The Frog, Rana Luteiventris, Maria Julia Tomaske Apr 2008

Efficiency And Accuracy Of Geometric Morphometries In The Analysis Of Intraspecific Variation In The Frog, Rana Luteiventris, Maria Julia Tomaske

Life and Environmental Sciences Undergraduate Theses

Geometric morphometric analysis was performed on three populations of Columbia Spotted Frog in Western Montana. The purpose was to examine the utility and sensitivity of this sort of analysis to morphometrically discriminate populations. Specimens were captured by hand, photographed, and released. Data was uploaded into a computer, landmarked, and analyzed. MANCOVA revealed significant differences between all three sample groups. Geometric morphometries was determined to be a reliable and accurate tool in distinguishing between separate populations of the same species, and determining the amount of variation existing among sample groups. It was also shown to be a sensitive tool, capable of ...


Survey Of Melanistic Garter Snakes By Traditional And Geometric Morphometries, James Van Leuven Apr 2008

Survey Of Melanistic Garter Snakes By Traditional And Geometric Morphometries, James Van Leuven

Life and Environmental Sciences Undergraduate Theses

Melanism in garter snake populations is a rare and ecologically important occurrence. Melanism can be used to study the function of coloration and the process of genetic drift. Coloration patterns of garter snakes may affect fitness level, influenced by predation and thermal efficiency. It is therefore of interest how and why the melanistic trait is acquired. Along the Missouri river near Townsend, MT, three garter snake morphotypes are found; Thamnophis elegans, Thamnophis sirtalis, and melanistic individuals inhabit the area. While T. sirtalis and T. elegans are well studied, little is known about the melanistic population in this area. The goal ...


Partial Isolation And Chemical Characterization Of Cytotoxic Compounds In Freshwater Sponges, Aaron Bertoni Apr 2008

Partial Isolation And Chemical Characterization Of Cytotoxic Compounds In Freshwater Sponges, Aaron Bertoni

Life and Environmental Sciences Undergraduate Theses

Marine sponges are known to produce or sequester compounds useful for defense against predation and to prevent fouling by parasites. Many of the compounds extracted from marine sponges have been shown to possess useful antitumoral properties. The present study investigates two species of freshwater sponges, Eunapius fragilis and Ephydatia muelleri, for possible cytotoxic compounds as determined by brine shrimp microassay. Results show that both species have cytotoxic compounds extractable in methanol and at least two cytotoxic compounds may be present in Eunapiusfragilis, both of which are extractable in hexane. The present study provides strong support for continued natural product research ...


Cytotoxic Activity In Freshwater Sponges, Buck Bania Apr 2007

Cytotoxic Activity In Freshwater Sponges, Buck Bania

Life and Environmental Sciences Undergraduate Theses

Cytotoxic compounds, presumably functioning in defense against predators, parasites, and infectious organisms, are produced or sequestered by a variety of invertebrate animals. Marine sponges, in particular, have proven to be a rich source of these compounds, and many of the compounds or derivatives of them are valuable pharmaceutically. Preliminary work suggested that freshwater sponges may also harbor cytotoxic/ such compounds. In order to examine this possibility, I extracted specimens two species of freshwater sponges, Ephydatia muelleri and Spongilla lacustris, with a range of solvents and tested for cytotoxic activity using a brine shrimp survival assay. I confirmed that freshwater sponges ...


Habitat Factors That Influence Oviposition Sites In Ecuadorian Glass Frogs, Mario Pizzini Apr 2007

Habitat Factors That Influence Oviposition Sites In Ecuadorian Glass Frogs, Mario Pizzini

Life and Environmental Sciences Undergraduate Theses

The numbers of amphibians are decreasing all over the globe, particularly in high elevation areas of the Andes. Glass frogs have been studied in tropical regions, but very little is known about the species in Ecuador. The objective of my study was to determine if habitat factors influence the selection of oviposition (egg laying) sites in Ecuadorian glass frogs in the Cordillera Oriental of the Andes Mountains, at the Yanayacu Biological Station. Two and a half km of one stream were selected as the study area because its bank vegetation and stream characteristics varied throughout its length. The stream was ...


Screening For Antibiotic, Antifungal And Antitumoral Compounds In Natural Products Extracted From Freshwater Sponges, Danielle Daehnke Apr 2006

Screening For Antibiotic, Antifungal And Antitumoral Compounds In Natural Products Extracted From Freshwater Sponges, Danielle Daehnke

Life and Environmental Sciences Undergraduate Theses

The secondary metabolites of marine sponges include many bioactive products (e.g., ara-C) that have found uses in the health field. Even though freshwater sponges share many characteristics with their marine cousins, little is known about their secondary metabolites. This study looks at the effects of the secondary metabolites extracted from three freshwater sponge species on the survival of bacteria, fungi and brine shrimp. It found that none of the extracts were toxic to the bacteria or fungi. Preliminary results did, however, show that all three species tested were active against the brine shrimp. This study suggests that the secondary ...


Wetland Characteristics Associated With Reproductive Activity Of Rana Sylvatica In Denali National Park, Alaska, Andrew Brown Apr 2005

Wetland Characteristics Associated With Reproductive Activity Of Rana Sylvatica In Denali National Park, Alaska, Andrew Brown

Life and Environmental Sciences Undergraduate Theses

Habitat characteristics greatly impact the distribution of amphibians. While Rana sylvatica has been extensively studied in Northeastern United States and Southeastern Canada, relatively little is known about the characteristics of its habitat in Alaska. Rana sylvatica is the northernmost amphibian in North America and the only one found in the study area. I studied the effects of habitat on reproductive activity of frogs near Wonder Lake in Denali National Park and Preserve, Alaska during the summer of 2004. R. sylvatica tadpoles were found in 98 of 219 randomly sampled wetland sites. A higher proportion of breeding activity was observed for ...


Immunocytochemical Localization Of Rab5 In Perialgal Vacuoles Of A Freshwater Sponge, Hannah Jarvis Apr 2005

Immunocytochemical Localization Of Rab5 In Perialgal Vacuoles Of A Freshwater Sponge, Hannah Jarvis

Life and Environmental Sciences Undergraduate Theses

Chlorella sp. is a green alga that exists in endosymbiotic relationships with a number of freshwater invertebrates including the sponge, Ephydatia muelleri. Algae enter sponge cells through phagocytosis, but unlike most cells taken up by this process, the algal cells are not degraded. The mechanism by which Chlorella escapes degradation is currently unknown. One hypothesis is that the vacuoles containing algal cells do not progress along the degradative pathway but instead are maintained in an immature, pre-lysosomal state, displaying the early endosomal marker Rab5. In order to test this hypothesis, I localized Rab5 in sponge cells using immunocytochemistry. Two antibodies ...


Localization Of The Late Endosome Marker Rab7 In The Freshwater Sponge Ephydatia Muelleri, Lindsay Carlile Apr 2005

Localization Of The Late Endosome Marker Rab7 In The Freshwater Sponge Ephydatia Muelleri, Lindsay Carlile

Life and Environmental Sciences Undergraduate Theses

The green alga, Chlorella, exists in a symbiotic relationship with the freshwater sponge, Ephydatia muelleri. Chlorella is taken up by phagocytosis and incorporated into phagosomes. However, the phagosomes containing the algae fail to fuse with lysosomes, resulting in a sustained symbiotic relationship. One possible explanation for the retention of algae in non-lysosomal vacuoles is that the phagosomes do not acquire the surface proteins needed for fusion with lysosomes. Among these proteins is Rab7, which has been suggested to regulate the transition from phagosome to lysosome. I hypothesized that the vacuole containing the symbiotic algae is early endosome-like and thus Rab7 ...


A Geometric Morphometric Analysis Of Tail Morphology In The Colombia Spotted Frog, Rana Lutieventris, Tim Mitchell Apr 2004

A Geometric Morphometric Analysis Of Tail Morphology In The Colombia Spotted Frog, Rana Lutieventris, Tim Mitchell

Life and Environmental Sciences Undergraduate Theses

Phenotypic plasticity is the result of environmental factors interacting with genes to influence the phenotype of individuals. Developing amphibian larvae may be influenced by the presence of tadpole predators. Rana lutieventris tadpoles were reared in the presence of a predator, presence of an injured conspecific, presence of both a mashed conspecific and predator, and a control of no predator or conspecific for a period of six weeks. Photographs were taken weekly to monitor tail growth. The photographs were digitized and the data was analyzed using geometric morphometries. Geometric morphometric software pooled the data, eliminated differences in size, position, and orientation ...


Olfaction And Maternal Behaviors In Rats, Ann Miner Apr 2004

Olfaction And Maternal Behaviors In Rats, Ann Miner

Life and Environmental Sciences Undergraduate Theses

The research on maternal behaviors and maternal responsiveness is extensive. Maternal responsiveness in rats consists of retrieval ofpups to the nest, grouping pups, building the nest, and licking and nursing of the pups. There are studies suggesting that audition, vision, olfaction, and vomeronasal (VNO) function play a role in the task of maternal responsiveness. Some research demonstrates that odors play no role in responsiveness while other research suggests odors are required (Alberts & Farrell 2002 b). The ability to recognize offspring has been credited to the olfactory epithelium as well as the VNO. The current research analyzed the role of the olfactory epithelium in offspring recognition and retrieval in female rats. Pups were placed in two treatment groups; one group was painted with citrus scented oil (containing volatile chemical odorants) on days two through six postpartum, the second group was painted with citrus oil on days ...


The Effects Of Hydroperiod On The Head Morphology Of Long-Toed Salamanders (Ambystoma Macrodactylum), Kathryn Menninger Apr 2004

The Effects Of Hydroperiod On The Head Morphology Of Long-Toed Salamanders (Ambystoma Macrodactylum), Kathryn Menninger

Life and Environmental Sciences Undergraduate Theses

Phenotypic plasticity has been observed in many organisms from mammals to plants. In particular amphibians are susceptible to plasticity before metamorphosis. Hydroperiod (the amount oftime water is present in a pond) is a significant factor in amphibian development. It has been indicated to cause phenotypic plasticity in the head morphology of salamanders. Cannibalistic and typical head morphologies have been recorded in the long-toed salamander Ambystoma macrodactylum. Larval salamanders in both short and long hydroperiod ponds were observed using macro-photography. Geometric morphometries was then used to analyze head shapes. The two sites were sampled throughout the summer of 2003 to view ...


The Effects Of Hydroperiod On The Morphology Of The Long-Toed Salamander Ambystoma Macrodactylum, Jesse Sherratt Apr 2003

The Effects Of Hydroperiod On The Morphology Of The Long-Toed Salamander Ambystoma Macrodactylum, Jesse Sherratt

Life and Environmental Sciences Undergraduate Theses

This study was conducted to determine whether salamander larvae of Ambystoma macrodactylum growing in short hydroperiod ponds develop a cannibalistic morphology while those growing in a long hydroperiod pond develop a typical morphology. Hand and dip net capturing techniques were used to collect the larvae. They were then preserved in formalin and stored in 95% ethanol solution. Six measurements were then taken: snout-vent length, head width at gills, head width at eyes, head width at jaws, head length, and interocular width. Snout-vent-length was then scaled to the other measurements to determine differences in body shape. Using an ANOVA statistical test ...


Wetland Characteristics Associated With Amphibian Presence In The Rocky Mountain Region, Sarah Mcavoy Apr 2003

Wetland Characteristics Associated With Amphibian Presence In The Rocky Mountain Region, Sarah Mcavoy

Life and Environmental Sciences Undergraduate Theses

Hydroperiod (the annual period that open water is available) is an important factor in studies of wetland ecology. It has been linked to amphibian development, distribution, and rates of survival as well as the presence of exotic species. However, hydroperiod is rarely measured directly. Typically wetland size is used as a surrogate measurement of hydroperiod. Recent studies suggest that wetland size may not be as closely related to hydroperiod as was previously thought. To investigate the role of hydroperiod and its relationship with other habitat parameters, I conducted a study of 23 wetlands located in west-central Montana. Each wetland was ...


The Effects Of The Presence Of Dragonfly Predators On The Morphology Of The Columbia Spotted Frog (Rana Luteiventris), Kyle Bodley Apr 2003

The Effects Of The Presence Of Dragonfly Predators On The Morphology Of The Columbia Spotted Frog (Rana Luteiventris), Kyle Bodley

Life and Environmental Sciences Undergraduate Theses

A laboratory experiment was preformed to determine the effects of predation on the morphological plasticity of the Columbia spotted frog (Rana luteiventris). Four treatments, each comprised of five replicates included predators, mashed conspecifics, predators and mashed conspecifics and a control group. Different morphological features were measured and a statistical analysis of these features was performed to determine the overall effect of predation. Predation, or the presence of chemical cues released from the predator or the skin of the injured amphibian, had a significant effect on muscle development. Specifically, tadpoles reared in the presence of predators or mashed conspecifics developed thicker ...


Immunocytochemical Localization Of Rab 5 In Freshwater Sponge Cells Containing Algal Endosymbionts, Bethany Wallace Apr 2002

Immunocytochemical Localization Of Rab 5 In Freshwater Sponge Cells Containing Algal Endosymbionts, Bethany Wallace

Life and Environmental Sciences Undergraduate Theses

The freshwater sponge, Ephydcitia muelleri, harbors symbiotic algae within its cells. These algae are taken up by phagoctyosis, but they fail to be broken down. One explanation for the lack of degradation is that their digestion is prevented at an unknown point along the phagocytic pathway. In an attempt to determine if the vacuoles containing algal endosymbionts had characteristics associated with early endosomes, I used immunocytochemistry to localize Rab 5 in sponge cells. Rab 5 is a protein that binds to membranes and functions in docking and fusion events early in the endocytic and phagocytic pathways. Immunoblots using antibody against ...


Age Distribution Patterns In A Small And A Large Population Of Spotted Frogs (Rana Luteiventris), Scott Davis Apr 2002

Age Distribution Patterns In A Small And A Large Population Of Spotted Frogs (Rana Luteiventris), Scott Davis

Life and Environmental Sciences Undergraduate Theses

Habitat fragmentation is one of the primary factors responsible for the decline of amphibians and has been shown to result in smaller and more isolated populations. In this study, a large and a small population of spotted frogs (Rana luteiventris) were studied in order to determine what effects, if any, patch size and isolation have on age structure and vital rates within populations of R. luteiventris. Individual frogs were captured, marked, and released. Skeletochronology was then used to determine individual age. Age structures were found to be significantly different between the populations, with the small population being composed mainly of ...


The Effect Of Predation On Tadpole Morphology Of The Spotted Frog (Rana Luteiventris), Reed Simons Apr 2002

The Effect Of Predation On Tadpole Morphology Of The Spotted Frog (Rana Luteiventris), Reed Simons

Life and Environmental Sciences Undergraduate Theses

A field experiment was performed to examine the effects of predation on the morphology of the spotted frog (Rana luteiventris). Seven ponds were randomly selected for survey in the Helena National Forest. Macroinvertebrate predators were counted and tadpoles were collected to measure morphological characteristics. Statistical analysis demonstrated that predation had a significant overall effect on tail morphology. Specifically, the higher number of predators in a pond, the longer the tail, the thicker the tail muscle, and the more narrow the mouth width of tadpoles. These induced changes presumably increase swimming speed, improving predator evasion. Interactions with other members of the ...


Microhabitat Selection And Growth Rate: A Comparison Between The Western Toad (Bufo Boreas) And The Spotted Frog (Rana Luteiventris), Jonathan Warner Apr 2002

Microhabitat Selection And Growth Rate: A Comparison Between The Western Toad (Bufo Boreas) And The Spotted Frog (Rana Luteiventris), Jonathan Warner

Life and Environmental Sciences Undergraduate Theses

Bufo boreas is a species of toad that has been undergoing extreme larval mortality. Several anthropogenic factors are known to aid in the decline, but less is known about the behavioral patterns and microhabitat influences on the decline. Tadpoles of Rana luteiventris and B. boreas were observed in the wild and data for temperature and depth selection were gathered over a three-month period. Tadpoles of B. boreas selected warmer waters and varied their depth, while tadpoles of R. luteiventris were shown to be more variable regarding temperature selection and less variable regarding depth selection. Even though the tadpoles of B ...


Spotted Frog (Rana Lutcivcntris) Dispersal In The Lower Lump Drainage, Jonathan Griffin Apr 2002

Spotted Frog (Rana Lutcivcntris) Dispersal In The Lower Lump Drainage, Jonathan Griffin

Life and Environmental Sciences Undergraduate Theses

Populations of amphibian species have been declining throughout the world. This factor is significant because amphibian population dynamics can be an indication of anthropogenic disturbance in the environment. The ability of adjacent populations to interact via dispersing individuals correlates with the overall resilience of a species. Landscape features coupled with varied seasonal needs are also factors that have an impact on dispersal rates. The objective of this study was to delineate dispersal patterns of Rana luteiventris with respect to temporal variations, spatial factors, individual disperser characteristics, and effects of mining effluent on dispersal. Gaining a better understanding of the patterns ...


Mtdna Analysis Of Sexual Dispersal Among Glacier National Park Grizzly Bears (Ursus Arctos), Joseph Chiovaro Apr 2002

Mtdna Analysis Of Sexual Dispersal Among Glacier National Park Grizzly Bears (Ursus Arctos), Joseph Chiovaro

Life and Environmental Sciences Undergraduate Theses

This study compared 369 base pairs of the hypervariable control region of mitochondrial DNA in 137 brown bears (71 males, 66 females) of Glacier National Park to investigate dispersal patterns between the sexes. Since females establish home ranges close to their birth sites, we hypothesized a stronger phylogeographic pattern among females. Contrary to our expectations, males exhibited stronger phylogeographic patterning with one haplotype in males occurring exclusively in northern regions of the park, while a second occurred exclusively in the south. No phylogeographic patterning was observed among females. Male dispersal patterns could be influenced by gene flow from regions contiguous ...


Modeling The Relationship Between Landscape Structure And Amphibian Breeding, John Kingston Apr 2000

Modeling The Relationship Between Landscape Structure And Amphibian Breeding, John Kingston

Life and Environmental Sciences Undergraduate Theses

Amphibians serve as indicators of the health of the environment in which they live. Identification of elements in the environment important for amphibian persistence can be used to construct a quantitative habitat model. This model can • provide information about the ecological requirements of a particular species. In this study, I examined the long-toed salamander (Ambystoma macrodactylum) and the spotted frog (Rana luteiventris) in the Lump Gulch Drainage in the Helena National Forest, Montana. Ponds were surveyed and various landscape features were measured. I found spotted frog breeding to be significantly associated with pond area and amount of submergent vegetation. Long-toed ...


Impact Of Stream Corridors On Breeding Populations Of Rana Luteiventris, Fredrick Bartoletti Apr 2000

Impact Of Stream Corridors On Breeding Populations Of Rana Luteiventris, Fredrick Bartoletti

Life and Environmental Sciences Undergraduate Theses

The biodiversity on Earth is in a state of decline. Because the full extent of the problem is not known, model systems are needed as environmental indicators. Amphibians are considered good indicators of wetland ecosystems. Many authors have agreed that a key factor driving population dynamics in a fragmented landscape is the ability of animals to move between habitat fragments. I studied the effects that a stream corridor has on the patch occupancy of breeding populations of western spotted frog (Rana luteiventris). I quantified landscape and habitat parameters in both Lump Gulch and Gravely Range landscapes. The Lump Gulch landscape ...


Kin Recognition In Tadpoles Of Rana Luteiventris Reared In Different Densities, Douglas Barber Apr 2000

Kin Recognition In Tadpoles Of Rana Luteiventris Reared In Different Densities, Douglas Barber

Life and Environmental Sciences Undergraduate Theses

After rearing Rana luteiventris tadpoles at high (80-100/L) and low (2/L) densities, I tested tadpoles for kin discriminatory behavior in a standardized choice tank. To test whether density manipulations influenced growth, I measured tadpole mass at metamorphosis. Results suggest that density manipulations were not effective, as tadpole mass at metamorphosis was not different between treatments. However, tadpoles exhibited kin discriminatory behavior irrespective of density treatments. My results suggest spotted frog tadpoles exhibit kin discriminatory behavior, although it is still unknown whether rearing conditions may influence such behavior.