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Microbiology

1986

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Full-Text Articles in Life Sciences

End-Products Of Anaerobic Chitin Degradation By Salt-Marsh Bacteria As Substrates For Dissimilatory Sulfate Reduction And Methanogenesis, Jn Boyer Dec 1986

End-Products Of Anaerobic Chitin Degradation By Salt-Marsh Bacteria As Substrates For Dissimilatory Sulfate Reduction And Methanogenesis, Jn Boyer

VIMS Articles

The anaerobic pathway of chitin decomposition by chitinoclastic bacteria was examined with an emphasis on end product coupling to other salt marsh bacteria. Actively growing chitinoclastic bacterial isolates produced primarily acetate, H2, and CO2 in broth culture. No sulfate-reducing or methanogenic isolates grew on chitin as sole carbon source or produced any measurable degradation products. Mixed cultures of chitin degraders with sulfate reducers resulted in positive sulfide production. Mixed cultures of chitin-degrading isolates with methanogens resulted in the production of CH4 with reductions in headspace CO2 and H2. The combination of all three metabolic types resulted in the simultaneous production ...


Immunological Relationships Among Proteins Making Up The Bacillus Thuringiensis Subsp. Israelensis Crystalline Toxin, Mary Ann Pfannenstiel, Graham A. Couche, Elise J. Ross, Kenneth W. Nickerson Oct 1986

Immunological Relationships Among Proteins Making Up The Bacillus Thuringiensis Subsp. Israelensis Crystalline Toxin, Mary Ann Pfannenstiel, Graham A. Couche, Elise J. Ross, Kenneth W. Nickerson

Papers in Microbiology

The immunological relationships among the proteins of the mosquito larvicidal toxin produced by Bacillus thuringiensis subsp. israelensis have been investigated by using polyclonal antisera specific for the 28-, 70-, and 135-kilodalton proteins. Each of these proteins was immunologically distinct. There was no cross-reaction among the three proteins and the two non-homologous antisera. Treatment of toxin proteins with larval gut enzymes for 20 h identified protease-resistant domains at approximately 65, 38, and 22 kilodaltons. Similar domains were generated by treatment with trypsin and chymotrypsin. Our immunological and kinetic data indicate that the 28-kilodalton protein is degraded successively to protein bands at ...


Analysis Of Mosquito Larvicidal Potential Exhibited By Vegetative Cells Of Bacillus Thuringiensis Subsp. Israelensis, Corey J. Walther, Graham A. Couche, Mary Ann Pfannenstiel, Sara E. Egan, Lisa A. Bivin, Kenneth W. Nickerson Oct 1986

Analysis Of Mosquito Larvicidal Potential Exhibited By Vegetative Cells Of Bacillus Thuringiensis Subsp. Israelensis, Corey J. Walther, Graham A. Couche, Mary Ann Pfannenstiel, Sara E. Egan, Lisa A. Bivin, Kenneth W. Nickerson

Papers in Microbiology

Vegetative Bacillus thuringiensis subsp. israelensis cells (6 x 105/ml) achieved 100% mortality of Aedes aegypti larvae within 24 h. This larvicidal potential was localized within the cells; the cell-free supernatants did not kill mosquito larvae. However, they did contain a heat-labile hemolysin which was immunologically distinct from the general cytolytic (hemolytic) factor released during solubilization of B. thuringiensis subsp. israelensis crystals. The larvicidal potential of the vegetative cells was not due to poly-3-hydroxybutyrate. Instead, it correlated with the ability of vegetative cells to sporulate during the bioassays. No toxicity was observed when bioassays were conducted in the presence ...


Presence Of A Polyadenylated Rna Fragment Encoding The Membrane Domain For Immunoglobulin Alpha Chain Indicates That Mrnas For Both Secreted And Membrane-Bound Alpha Chains Can Be Produced From The Same Rna Transcript, Janet Stavnezer Aug 1986

Presence Of A Polyadenylated Rna Fragment Encoding The Membrane Domain For Immunoglobulin Alpha Chain Indicates That Mrnas For Both Secreted And Membrane-Bound Alpha Chains Can Be Produced From The Same Rna Transcript, Janet Stavnezer

Women’s Health Research Faculty Publications

RNA blotting was employed to examine polyadenylated immunoglobulin alpha chain RNAs in a B lymphoma synthesizing membrane-bound and secretory IgA and in a hybridoma which synthesizes predominantly secretory IgA. Both cell lines were derived from the I.29 lymphoma and expressed the identical heavy chain variable region gene. In addition to the predicted mRNA precursors, four novel species of polyadenylated alpha RNAs were detected. The presence of a RNA species which was too large to have the same 3' end as the largest mRNA for membrane-bound alpha chain (alpha m) implied that transcription continued past the alpha m poly(A ...


Production Of Acquired Immunodeficiency Syndrome-Associated Retrovirus In Human And Nonhuman Cells Transfected With An Infectious Molecular Clone, Akio Adachi, Howard E. Gendelman, Scott Koenig, Thomas Folks, Ronald Willey, Arnold Rabson, Malcom A. Martin Aug 1986

Production Of Acquired Immunodeficiency Syndrome-Associated Retrovirus In Human And Nonhuman Cells Transfected With An Infectious Molecular Clone, Akio Adachi, Howard E. Gendelman, Scott Koenig, Thomas Folks, Ronald Willey, Arnold Rabson, Malcom A. Martin

Virology Papers

We constructed an infectious molecular clone of acquired immunodeficiency syndrome-associated retrovirus. Upon transfection, this clone directed the production of infectious virus particles in a wide variety of cells in addition to human T4 cells. The progeny, infectious virions, were synthesized in mouse, mink, monkey, and several human non-T cell lines, indicating the absence of any intracellular obstacle to viral RNA or protein production or assembly. During the course of these studies, a human colon carcinoma cell line, exquisitely sensitive to DNA transfection, was identified.


Comparison Of Membrane Filter, Multiple-Fermentation-Tube, And Presence-Absence Techniques For Detecting Total Coliforms In Small Community Water Systems., Nicholas J. Jacobs, Wanda L. Zeigler, Frank C. Reed, Therese A. Stukel, Eugene W. Rice May 1986

Comparison Of Membrane Filter, Multiple-Fermentation-Tube, And Presence-Absence Techniques For Detecting Total Coliforms In Small Community Water Systems., Nicholas J. Jacobs, Wanda L. Zeigler, Frank C. Reed, Therese A. Stukel, Eugene W. Rice

Open Dartmouth: Faculty Open Access Scholarship

Methods for detecting total coliform bacteria in drinking water were compared using 1,483 different drinking water samples from 15 small community water systems in Vermont and New Hampshire. The methods included the membrane filter (MF) technique, a 10-tube fermentation tube (FT) technique, and the presence-absence (P-A) test.


Purification Of Anthrax Toxin Protective Antigen Component And Characterization Of Its Binding Interaction With Bovine Kidney Cells, Daniel Dalton Martin May 1986

Purification Of Anthrax Toxin Protective Antigen Component And Characterization Of Its Binding Interaction With Bovine Kidney Cells, Daniel Dalton Martin

All Graduate Theses and Dissertations

Protective antigen component of B. anthracis toxin was produced and purified to the >99% level. Toxin was purified from culture supernatant utilizing concentration and liquid chromatography techniques. Purity was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

The purified protective antigen retained biological and antigenic activity as evidenced respectively by lethality in Fischer 344 rats when injected in combination with lethal factor, and by positive results on the Ouchterlony double diffussion assay.

Radioiodinated protective antigen was used both in the in vivo and the in vitro experiments. In vivo distribution of labelled protective antigen was determined in Fischer 344 rats. Assay ...


Linear Cell Mass Increase During The Cell Cycle Of Escherichia Coli, Patricia Winslow Apr 1986

Linear Cell Mass Increase During The Cell Cycle Of Escherichia Coli, Patricia Winslow

Life and Environmental Sciences Undergraduate Theses

Linear increase of cell mass of undivided cells was determined during the division cycle of Escherichia coli B/rA. Synchronous growth was obtained by selection of cells from exponentially growing populations after velocity sedimentation in sucrose density gradients. Cell mass was calculated as a product of mean cell volume and buoyant density that were measured at intervals during synchronous growth. A liner increase in cell mass was observed in two different growth media which had doubling times of 40.0 and 30.1 minutes. The results support the theory of constant linear increase of cell mass throughout the division cycle.


Isolation & Partial Characterization Of The Capsule Produced By Neisseria Gonorrhoeae, Patricia Sullivan Apr 1986

Isolation & Partial Characterization Of The Capsule Produced By Neisseria Gonorrhoeae, Patricia Sullivan

Life and Environmental Sciences Undergraduate Theses

The capsule of Neisseria gonorrhoeae strains JS1 and JS3 was extracted according to the procedure of Wolfgang Casper (1937). The extracted products were biologically and biochemically characterized. The extracts were comprised primarily of carbohydrate and other constituents detected are ribonucleic acids, protein, 1ipopolysaccharide, phosphorus, and 2-keto-3-deoxyoctonate. The presence of these impurities has greatly interfered with the identification of the specific polysaccharide components. Examination of Neisseria gonorrhoeae by scanning electron microscopy indicated that extracellular material was stabilized by low temperatures. In order to maximize the amount of extracted polysaccha ride, the bacterial suspensions were refrigerated whenever possible during the purification procedure.


Cell Growth Of Saccharomyces Cerevisiae, Miguel Schmitz Apr 1986

Cell Growth Of Saccharomyces Cerevisiae, Miguel Schmitz

Life and Environmental Sciences Undergraduate Theses

From an exponentially growing yeast culture, we obtained a synchronized culture using velocity gradient centrifugation. At five minute intervals, we determined the average cell buoyant density and the peak frequency cell volume to calculate the average cell mass at each of these time intervals. We found that the average yeast cell increases in mass at an exponential rate.


A Study Of The Determinants On Gp70, A Viral Envelope Glycoprotein, To Which Neutralizing Monoclonal Antibodies Bind, Swend Holland Apr 1986

A Study Of The Determinants On Gp70, A Viral Envelope Glycoprotein, To Which Neutralizing Monoclonal Antibodies Bind, Swend Holland

Life and Environmental Sciences Undergraduate Theses

Lake Casitas Ecotropic virus causes a hind limb paralysis when injected into newborn mice. The long term goal is to select mutants of the virus using monoclonal antibodies and mutagens. Two of the monoclonal antibodies produced against this virus are neutralizing and also bind to the protein part of gp70, a viral envelope glycoprotein. Because of this, they can be used to select mutant viruses. There is no significant binding inhibition between monoclonal 704 and either of the two neutralizing monoclonals. Therefore, monoclonal 704 can be used in a focal-immuno-assay with the neutralizing monoclonals to locate mutant viruses.


Adp-Ribosylation Of Bsa By Pertussigen Follows The Same Mechanism As Adp-Ribosylation Of Transducine, John Cabrera Apr 1986

Adp-Ribosylation Of Bsa By Pertussigen Follows The Same Mechanism As Adp-Ribosylation Of Transducine, John Cabrera

Life and Environmental Sciences Undergraduate Theses

Pertussigen is a toxin responsible for the ADP-ribosylation of the Adenylate cyclase system inhibitory component. It has been verified through polyacrylamide gel electrophoresis that the ADP-ribosylation occurs only if the reaction mixture is supplemented with ATP, NAD and DTT. There is also a linear relationship between time and the amount of protein which is ADP ribosylated. Pertussigen can also ADP-ribosylize Bovine Serum Albumin (BSA) which offers researchers an inexpensive way to study the action of pertussigen.


The Effects Of Varying Nacl Concentrations On The Cultures Of The Rotifer, Brachionus Plicatilis, Maureen Mccormack Apr 1986

The Effects Of Varying Nacl Concentrations On The Cultures Of The Rotifer, Brachionus Plicatilis, Maureen Mccormack

Life and Environmental Sciences Undergraduate Theses

Populations of the rotifer, Brachionus, plicatilis. were cultured in medium containing various concentrations (2, 5, 10, and 20 gl-1) of NaCl. Comparisons of the life history characteristics show that the media containing 2, 5, and 10 gl-1 of NaCl sustain positive population growth; the media containing 10 gl-1 of NaCl has the highest levels of survivorship and reproduction. The medium containing 2 gl-1 of NaCl is inadequate for sustaining a population of Brachionus plicatilis indefinitely.


Construction Of Escherichia Coli Mapping Strains And Mapping Of The Lysine-Decarboxylase Gene Cad A, Laura Bray Apr 1986

Construction Of Escherichia Coli Mapping Strains And Mapping Of The Lysine-Decarboxylase Gene Cad A, Laura Bray

Life and Environmental Sciences Undergraduate Theses

The Escherichia coli lysine-decarboxylase gene, cadA, was mapped using the mutant form and two and three factor Crosses. The three point analysis showed the gene order of the markers to be me1A - cadA - groE. Two factor crosses yielded cotransduction frequencies which were compared to results from other mapping experiments. After considering the length of insertion element TnlO, the agreement with pub dished' data was very good. Map distances were calculated from the cotransduction frequencies. The significance of this experiment lies in the fact that it mapped the cadA gene more closely and conclusively than had been done previously. Markers on ...


Tropism Of Sheep Lentiviruses For Monocytes: Susceptibility To Infection And Virus Gene Expression Increase During Maturation Of Monocytes To Macrophages, Howard E. Gendelman, Opendra Narayan, Suzanne Kennedy-Stoskopf, Peter G. E. Kennedy, Zahra Ghotbi, J. E. Clements, Jeff Stanley, Gholam Pezeshkpour Apr 1986

Tropism Of Sheep Lentiviruses For Monocytes: Susceptibility To Infection And Virus Gene Expression Increase During Maturation Of Monocytes To Macrophages, Howard E. Gendelman, Opendra Narayan, Suzanne Kennedy-Stoskopf, Peter G. E. Kennedy, Zahra Ghotbi, J. E. Clements, Jeff Stanley, Gholam Pezeshkpour

Virology Papers

Visna lentiviruses have a natural tropism for cells of the macrophage lineage of sheep and goats, but virus replication in these cells in vivo is restricted so that only small quantities of virus are produced. One restricting factor suggested in previous studies is that virus replication is dependent on the maturity of the cells: the more mature the cell, the less restrictive the replication of the virus. Since monocytes in peripheral blood are precursors of macrophages, we investigated the effect of cell maturation on virus replication under limited control conditions in vitro by inoculating blood leukocytes with virus and retarding ...


Absence Of A Structural Basis For Intracellular Recognition And Differential Localization Of Nuclear And Plasma Membrane-Associated Forms Of Simian Virus 40 Large Tumor Antigen., Donald L. Jarvis, Charles N. Cole, Janet S. Butel Mar 1986

Absence Of A Structural Basis For Intracellular Recognition And Differential Localization Of Nuclear And Plasma Membrane-Associated Forms Of Simian Virus 40 Large Tumor Antigen., Donald L. Jarvis, Charles N. Cole, Janet S. Butel

Open Dartmouth: Faculty Open Access Scholarship

The simian virus 40 large tumor antigen (T-ag) is found in both the nuclei (nT-ag) and plasma membranes (mT-ag) of simian virus 40-infected or -transformed cells. It is not known how newly synthesized T-ag molecules are recognized, sorted, and transported to their ultimate subcellular destinations. One possibility is that these events depend upon structural differences between nT-ag and mT-ag. To test this possibility, we compared the structures of nT-ag and mT-ag from simian virus 40-infected cells. No differences between the two forms of T-ag were detected by migration in polyacrylamide gels, by Staphylococcus aureus V8 partial proteolytic mapping of methionine- ...


Model For The Distribution Of Sulfate Reduction And Methanogenesis In Freshwater Sediments, Derek Lovley, Michael J. Klug Jan 1986

Model For The Distribution Of Sulfate Reduction And Methanogenesis In Freshwater Sediments, Derek Lovley, Michael J. Klug

Derek Lovley

No abstract provided.


Cyanonews (Vol. 2, No. 3, December 1986), Jeff Elhai Jan 1986

Cyanonews (Vol. 2, No. 3, December 1986), Jeff Elhai

CyanoNews

CyanoNews was a newsletter that served the cyanobacteriological community from 1985 to 2003, with content provided by readers (sort of a blog before there were blogs). The newsletter reported new findings from the lab, summaries of recent meetings (often provided by graduate students and post-docs entering the field), positions sought or available, life transitions, a compendium of recent cyanobacteria-related articles, and other items of interest to those who study cyanobacteria.


Cyanonews (Vol. 2, No. 2, July 1986), Jeff Elhai Jan 1986

Cyanonews (Vol. 2, No. 2, July 1986), Jeff Elhai

CyanoNews

CyanoNews was a newsletter that served the cyanobacteriological community from 1985 to 2003, with content provided by readers (sort of a blog before there were blogs). The newsletter reported new findings from the lab, summaries of recent meetings (often provided by graduate students and post-docs entering the field), positions sought or available, life transitions, a compendium of recent cyanobacteria-related articles, and other items of interest to those who study cyanobacteria.


Cyanonews (Vol. 2, No. 1, March 1986), Jeff Elhai Jan 1986

Cyanonews (Vol. 2, No. 1, March 1986), Jeff Elhai

CyanoNews

CyanoNews was a newsletter that served the cyanobacteriological community from 1985 to 2003, with content provided by readers (sort of a blog before there were blogs). The newsletter reported new findings from the lab, summaries of recent meetings (often provided by graduate students and post-docs entering the field), positions sought or available, life transitions, a compendium of recent cyanobacteria-related articles, and other items of interest to those who study cyanobacteria.


The Use Of Monoclonal And Polyclonal Antibodies To Identify Escherichia Coli , Charles William Kaspar Jan 1986

The Use Of Monoclonal And Polyclonal Antibodies To Identify Escherichia Coli , Charles William Kaspar

Retrospective Theses and Dissertations

Coagglutination tests with polyclonal antibodies were developed for the detection of Escherichia coli [beta]-galactosidase (GAL), [beta]-glucuronidase (GUD), and glutamate decarboxylase (GAD) in cell lysates. All three enzymes were detected in 93% of the E. coli tested; two of the three enzymes were detected in the remaining 7%. Among 42 non-E. coli, two (5%) were agglutinated by all three anti-enzyme conjugates, two (5%) were agglutinated by two conjugates, six (14%) were agglutinated by a single conjugate, and 32 (76%) were not agglutinated by any of the conjugates;Enzyme-capture assays with polyclonal antibodies detected the presence of GAL in seven ...


Molecular Cloning Of Cdna To Soybean Mosaic Virus Rna, Louis Michael Mansky Jan 1986

Molecular Cloning Of Cdna To Soybean Mosaic Virus Rna, Louis Michael Mansky

Retrospective Theses and Dissertations

No abstract provided.


The Role Of Iron In The Pathogenesis Of Escherichia Coli Septicemia Of Turkeys (Meleagris Gallopavo) , Carole Ann Bolin Jan 1986

The Role Of Iron In The Pathogenesis Of Escherichia Coli Septicemia Of Turkeys (Meleagris Gallopavo) , Carole Ann Bolin

Retrospective Theses and Dissertations

The effect of inoculation with Escherichia coli on serum iron concentrations of turkeys and the effect of exogenous iron as ferric ammonium citrate, on E. coli septicemia in turkeys were determined. Inoculation of air sacs with E. coli produced hypoferremia in 18-day-old turkeys. Administration of iron with E. coli significantly increased mortality, frequency and degree of bacteremia, and severity of lesions in inoculated turkeys, compared with those turkeys given E. coli but not given iron. Similar results were seen whether iron was inoculated at the same location as E. coli or at a different location;Rabbit antiserum was prepared against ...


Characterization Of The Serotypes And Outer Membrane Protein Profiles Of Haemophilus Pleuropneumoniae Isolates From Midwestern Swine, And The Antibody Response Of Swine To H. Pleuropneumoniae Outer Membrane Components , Vicki J. Rapp Jan 1986

Characterization Of The Serotypes And Outer Membrane Protein Profiles Of Haemophilus Pleuropneumoniae Isolates From Midwestern Swine, And The Antibody Response Of Swine To H. Pleuropneumoniae Outer Membrane Components , Vicki J. Rapp

Retrospective Theses and Dissertations

Haemophilus pleuropneumoniae isolates were characterized biochemically, by serotyping, and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of enriched outer membrane protein (OMP) preparations. Serotyping of 141 isolates from Iowa and Illinois swine by rapid slide agglutination (RSA) and indirect fluorescent antibody (IFA) tests indicated an overall incidence of serotype 5, 55.3%; serotype 1, 34.0%; serotype 7, 7.8%; and nontypable, 2.8%. There was good correlation between the two test procedures; however, 87.2% of the isolates could be typed by RSA, and only 66.0% by IFA. The profiles of Sarkosyl-insoluble, OMP-enriched preparations of H. pleuropneumoniae were ...


Comparison Between Mycoplasma Bovoculi Strains By Electrophoretic And Immunoblotting Techniques: Identification Of Membrane Proteins Involved In Adherence , Barik A. Salih Jan 1986

Comparison Between Mycoplasma Bovoculi Strains By Electrophoretic And Immunoblotting Techniques: Identification Of Membrane Proteins Involved In Adherence , Barik A. Salih

Retrospective Theses and Dissertations

Protein profiles of six Mycoplasma bovoculi strains obtained from electrophoretic analysis (SDS-PAGE) revealed a close relatedness among these strains. However, the antigenic properties of these proteins varied. Proteins separated on SDS-PAGE gels and transferred to nitrocellulose paper showed strain specific and conserved antigens by western blot analysis when reacted with hyperimmune rabbit serum developed against strain FS8-7 of M. bovoculi. Monoclonal antibodies developed against the same strain recognized a protein band of 94,000 molecular weight found in all strains examined. This band, designated p94 was also detected by sera from calves experimentally exposed to M. bovoculi by ocular instillation ...


Interactions Between Mycoplasma Hyopneumoniae And The Porcine Immune System , Serge Jacques Messier Jan 1986

Interactions Between Mycoplasma Hyopneumoniae And The Porcine Immune System , Serge Jacques Messier

Retrospective Theses and Dissertations

Mycoplasmal pneumonia of swine (MPS), caused by Mycoplasma hyopneumoniae, has been identified as a major source of loss of revenue for swine producers all over the world. The nature of microscopic lesions of MPS strongly indicates that the immune system is involved in the development of these lesions. Better knowledge about the interactions between M. hyopneumoniae and the porcine immune system should lead to a better understanding of the pathogenesis of MPS;The nonspecific mitogenic effect of M. hyopneumoniae membranes for swine lymphocytes and the possible involvement of this effect in development of lesions in vivo was evaluated by intratracheal ...


Studies On The Epidemiology And Control Of Rabies , Mohammed Aboud Muhsin Jan 1986

Studies On The Epidemiology And Control Of Rabies , Mohammed Aboud Muhsin

Retrospective Theses and Dissertations

The work described in this dissertation falls into two main parts;Firstly, the epidemiology of rabies in Iowa is discussed in the first three sections. A retrospective study has shown a close association between rabies in skunks and rabies in domestic and other wild animals. Skunks were recognized as the main reservoir hosts to be considered in planning and targeting control measures in all species. The epidemiology of rabies in raccoons was investigated in two geographical locations. In both locations, there was a correlation in this species between the sero-positive rate and age as well as between the sero-positive rate ...


Plant Viruses., R. A.C. Jones Jan 1986

Plant Viruses., R. A.C. Jones

Experimental Summaries - Plant Research

Cucumber mosaic virus in legumes, 85A024, 85A122, 85A123, 85A153, 85A163, 85A217, 85A219. Alfalfa mosaic virus in annual medics.


Degradation Of Human Anaphylatoxin C3a By Rat Peritoneal Mast Cells: A Role For The Secretory Granule Enzyme Chymase And Heparin Proteoglycan, James Edmund Gervasoni Jr Jan 1986

Degradation Of Human Anaphylatoxin C3a By Rat Peritoneal Mast Cells: A Role For The Secretory Granule Enzyme Chymase And Heparin Proteoglycan, James Edmund Gervasoni Jr

Theses and Dissertations

Purified human C3a was iodinated (125I-C3a) and used to study the interaction of labeled peptide with rat peritoneal mast cells (RMC). Cellular binding of 125I-C3a occurred within 30 sec, followed by a rapid dissociation from the cell. Both the binding of 125I-C3a and the rate of dissociation from the cell were temperature dependent. At 0°C, the binding of 125I-C3a was increased and the rate of dissociation reduced, as compared to 37°C. Once 125I-C3a was exposed to RMC, it lost the ability to rebind to a second batch of RMC. Analysis of the supernatants ...


Characterization Of A Staphylococcal Trimethoprim Resistance Gene, Jerald Preston Coughter Jan 1986

Characterization Of A Staphylococcal Trimethoprim Resistance Gene, Jerald Preston Coughter

Theses and Dissertations

Trimethoprim resistance(Tp r) is encoded by conjugative plasmids in clinically significant staphylococcal isolates. Two genetically and physically similar plasmids from S. aureus, pG01 and pGOS, have Tp r genes that map in different locations on these plasmids. In order to study the relatedness of the Tp r genes and their products to other known Tp r genes, a 1.2 kb fragment of pG01 and a 4.2 kb fragment of pGOS were cloned in E. coli and used as probes for in situ filter hybridization experiments.

A 500 base pair subclone of the original 1.2 kb fragment ...