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Properties Of Hiv-1 Env And Human Seminal Fluid That Determine Virus Inhibition By Antibodies And Microbicides, Jacklyn Johnson Aug 2019

Properties Of Hiv-1 Env And Human Seminal Fluid That Determine Virus Inhibition By Antibodies And Microbicides, Jacklyn Johnson

Theses and Dissertations

Human immunodeficiency virus type 1 (HIV-1) establishes a persistent infection that leads to acquired immunodeficiency syndrome (AIDS). Approximately 36 million people worldwide are living with HIV-1, which is commonly acquired through sexual contact. Antiviral therapies control disease progression, but do not eliminate this virus from the host. Thus, global efforts are focused on developing vaccines that prevent HIV-1 transmission. Such vaccines are based on eliciting the production of protective antibodies that target the envelope glycoproteins (Envs) of this virus. Unfortunately, HIV-1 immunization trials have shown limited efficacy. A better understanding of the antibody-mediated inactivation process is needed to improve vaccine ...


Investigation Of Microbiota In Health And Disease Of Poultry, Bishnu Adhikari Aug 2019

Investigation Of Microbiota In Health And Disease Of Poultry, Bishnu Adhikari

Theses and Dissertations

The microbiotas play vital roles in health and diseases of both humans and animals. 16S rRNA genes sequence analysis is one of the most popular and commonly used methods in the analysis of microbiotas associated with hosts. In this dissertation, the microbiotas of chickens (broilers, breeders, and layers) and turkeys were evaluated by 16S rRNA gene sequencing. Characterization of the culturable subpopulations of Lactobacillus in the chicken gut can serve as a valuable resource for probiotic development. In Chapter 2, Lactobacillus subpopulations recovered on MRS from chicken gut were defined comprehensively for the first time using 16S rRNA gene profiling ...


Flavobacterium Gliding Motility: From Protein Secretion To Cell Surface Adhesin Movements, Joseph Johnston Aug 2019

Flavobacterium Gliding Motility: From Protein Secretion To Cell Surface Adhesin Movements, Joseph Johnston

Theses and Dissertations

Flavobacterium johnsoniae exhibits rapid gliding motility over surfaces. At least twenty genes are involved in this process. Seven of these, gldK, gldL, gldM, gldN, sprA, sprE, and sprT encode proteins of the type IX protein secretion system (T9SS). The T9SS is required for surface localization of the motility adhesins SprB and RemA, and for secretion of the soluble chitinase ChiA. This thesis demonstrates that the gliding motility proteins GldA, GldB, GldD, GldF, GldH, GldI and GldJ are also essential for secretion. Cells with mutations in the genes encoding any of these seven proteins had normal levels of gldK mRNA but ...


Characterization Of Microtubule Organizing Centers In The Genus Protostelium, Including Evolutionary Implications, Ethan Taylor Ozment May 2019

Characterization Of Microtubule Organizing Centers In The Genus Protostelium, Including Evolutionary Implications, Ethan Taylor Ozment

Theses and Dissertations

Microtubule organizing centers (MTOCs) are cellular regions of microtubule nucleation. The best known MTOCs are those associated with the centrosome, but several non-centrosomal MTOCs are known in eukaryotes, especially in land plants. MTOCs are poorly characterized across the breadth of amoebozoan diversity, but are well-known in certain amoebozoan lineages, including the genus of protosteloid slime molds Protostelium. The structure of the MTOC is known for two non-ciliated species, P. nocturnum and P. mycophaga, as well as P. aurantium, which can reversibly become ciliated under appropriate conditions. P. nocturnum and P. mycophaga have acentriolar centrosomal MTOCs while P. aurantium has a ...


Aqueous Ozone Inactivation Of Viruses And Bacteria On Biotic And Abiotic Surfaces, Cailin Dawley Dec 2018

Aqueous Ozone Inactivation Of Viruses And Bacteria On Biotic And Abiotic Surfaces, Cailin Dawley

Theses and Dissertations

Produce is susceptible to contamination by foodborne pathogens. Food service establishments utilize sanitizing agents to reduce microbes on produce surfaces. The research objectives were to evaluate the efficacy of aqueous ozone 1) on the inactivation of viruses and bacteria on produce; 2) on the inactivation of viruses on stainless steel; and 3) against viruses in association with bacteria on produce surfaces. For objective 1, Boston bibb lettuce and cherry tomatoes were spot inoculated with a cocktail of viruses (murine norovirus (MNV) and MS2 bacteriophage) or bacteria (Enterobacter cloacae and Bacillus cereus) and washed for 40 min with samples taken every ...


Assessment Of Salmonella Enterica Biofilms And Expression Differences Among Serovars, Zhaohao Shi Dec 2018

Assessment Of Salmonella Enterica Biofilms And Expression Differences Among Serovars, Zhaohao Shi

Theses and Dissertations

Biofilms are communities of microorganisms associated by a matrix of extracellular polymers. In this state, microorganisms occupy an ecological niche distinct from their free-floating, planktonic counterparts. Also, biofilm bacteria become biologically unique as they form communities and lose motility. The acquisition of these physiological attributes enables the biofilm to persist through harsh environmental conditions, including antimicrobial induced stress and to resist sanitization efforts. Because of these features, biofilms can rapidly disseminate across numerous surfaces and as they establish, become challenging to remove. This is a particular issue for the food industry as processing plants offer favorable conditions for biofilm formation ...


The Antimicrobial Properties Of Honey And Their Effect On Pathogenic Bacteria, Shreena Himanshu Mody Dec 2018

The Antimicrobial Properties Of Honey And Their Effect On Pathogenic Bacteria, Shreena Himanshu Mody

Theses and Dissertations

Honey has been used to heal wounds since ancient times. There are many references in ancient literature that cite honey for its medicinal uses. It is used as an alternative agent to cure infections of wounds, burns, ulcers etc. Researchers have shown some of the antimicrobial properties of honey when used as an ointment. When applied to an affected area, it helps to promote the growth of healthy tissue. One of the factors on which the quality of the honey depends, is its geographical origin. Based on the location, honey types can vary as much as 100-fold from each other ...


Galleria Mellonella As An Alternate Infection Model For Burkholderia Species And A Comparison Of Suspension And Surface Test Methods For Evaluating Sporicidal Efficacy, Joseph D. Thiriot Dec 2018

Galleria Mellonella As An Alternate Infection Model For Burkholderia Species And A Comparison Of Suspension And Surface Test Methods For Evaluating Sporicidal Efficacy, Joseph D. Thiriot

Theses and Dissertations

Melioidosis is a neglected tropical disease that continues unabated in many countries, particularly in Southeast Asia. There is no vaccine and antimicrobial treatment is expensive and complicated. Virulence models are important tools used to investigate genes involved in pathogenesis. Galleria mellonella is the larvae of the wax worm moth that has been used to model various infections. Based on previous studies, we attempted to establish an infection model using Burkholderia pseudomallei and Burkholderia thailandensis, a related species which is avirulent in humans. Injections of various forms of these species (fresh and frozen) were used to develop Kaplan-Meier plots. We also ...


Semen Exosomes: Intrinsic Inhibitors Of Hiv-1 Infection, Jennifer Lynn Welch Dec 2018

Semen Exosomes: Intrinsic Inhibitors Of Hiv-1 Infection, Jennifer Lynn Welch

Theses and Dissertations

Exosomes are cell-derived vesicles that circulate in bio-fluids and enclose cell-associated cargo, producing various consequences in intercellular communication and may contribute to microbial pathogenesis. Exosomes are similar in composition to enveloped viruses, making differentiation between exosomes and enveloped viruses difficult. Yet, exosomes and enveloped viruses may vary considerably in function. Exosomes produced from infected cells may incorporate viral material as they are simultaneously produced with viruses and depending upon the cargo, contribute to disease pathogenesis. Conversely, exosomes from uninfected cells may contribute to protection from infection. Exosomes from breast milk, vaginal fluid, and semen of healthy donors protect against HIV-1 ...


Dissecting The Mechanism Of Action Of A Novel Antifungal Peptide, Cody Bullock Aug 2018

Dissecting The Mechanism Of Action Of A Novel Antifungal Peptide, Cody Bullock

Theses and Dissertations

There is an urgent need for novel treatments for Candida infections. The utility of antimicrobial peptides for antifungal therapy has garnered interest in recent years. One promising family of peptides is the Histatins, a family of naturally-occurring peptides secreted into the oral cavity that display antimicrobial activity. Histatin 5 is a twenty-four amino acid peptide with strong antifungal activity. Studies from our laboratory have identified a small histatin-derived peptide, KM29, that yields fungicidal activity 10-fold greater than Histatin 5 against multiple Candida species. Our laboratory has focused on understanding the mechanism of action of KM29 to further develop it as ...


Comparative Microbial Community Dynamics In A Karst Aquifer System And Proximal Surface Stream In Northwest Arkansas, Josue Rodriguez Aug 2018

Comparative Microbial Community Dynamics In A Karst Aquifer System And Proximal Surface Stream In Northwest Arkansas, Josue Rodriguez

Theses and Dissertations

Northwest Arkansas has well-developed karst systems, with numerous sinking streams and springs. Karst conduits make it easy for contaminants to enter groundwater systems, degrading the water quality and destroying fragile karst ecosystems. With the increase of urbanization, potential threats in the form of fecal contamination may prove to be more of a problem. The purpose of this research is to compare the difference between microbial communities within two different settings, a karst aquifer and a surface stream. The microbial communities within Blowing Spring Cave and Little Sugar Creek were detected and identified in water and sediment samples. Samples were also ...


Cultivating Ecosystems: Microbial Communities In Recirculating Aquaculture Systems, Ryan Bartelme Aug 2018

Cultivating Ecosystems: Microbial Communities In Recirculating Aquaculture Systems, Ryan Bartelme

Theses and Dissertations

Intensive cultivation of fish is necessary to meet future global market demands. Recirculating aquaculture systems (RAS) enable dense growth of fish, while occupying less space than traditional aquaculture farms. However, RAS often experience complications and high fish mortalities due to disease and improper waste management. In properly functioning systems, the microorganisms associated with fish (gut, scales) as well as those found in the system environment (water, component surfaces) remove waste and maintain fish health by discouraging growth of opportunistic pathogens. Previous RAS microbiome studies are small in scope, utilize coarse methods, and contain limited long-term spatial or temporal data. With ...


The Diversity Found Among Carbapenem-Resistant Bacteria, Galen Edward Card Jul 2018

The Diversity Found Among Carbapenem-Resistant Bacteria, Galen Edward Card

Theses and Dissertations

This work will look at two factors that add to the diversity of carbapenem resistant bacteria. First, it focuses on the diversity of carbapenemase resistance plasmids. 446 plasmids were characterized by size, gene content and replicon groups. We identified that on average, over 30% of the encoded proteins on each plasmid have an unknown function. Plasmid sizes ranged from 1.6kb to 500kb, with an average of around 100kb and median of 80kb. Additionally, six replicon groups account for 80% of all the carbapenemase resistance plasmids. We also highlight the lack of data available for carbapenemase carrying plasmids from bacterial ...


Bacteriophages For Treating American Foulbrood And The Neutralization Of Paenibacillus Larvae Spores, Thomas Scott Brady Jul 2018

Bacteriophages For Treating American Foulbrood And The Neutralization Of Paenibacillus Larvae Spores, Thomas Scott Brady

Theses and Dissertations

The causative agent of the most devastating honeybee disease, American foulbrood (AFB), is the spore-forming bacterium Paenibacillus larvae. To prevent AFB outbreaks beekeepers prophylactically treat their hives with antibiotics even though it decreases the overall health of uninfected hives. A new treatment for AFB is needed due to recent legislation against using antibiotics, antibiotic resistance developing in P. larvae, and the resilience of P. larvae spores. Bacteriophages, or phages, are an attractive alternative to traditional antibiotics because of their specificity and ability to evolve alongside their target bacterium. In this study, two phage cocktails were developed for the treatment of ...


Biomarker Analysis And Clinical Relevance Of Thymidine Kinase 1 In Solid And Hematological Malignancies, Evita Giraldez Weagel Jun 2018

Biomarker Analysis And Clinical Relevance Of Thymidine Kinase 1 In Solid And Hematological Malignancies, Evita Giraldez Weagel

Theses and Dissertations

Despite the global effort to discover and improve ways to detect, treat, and monitor cancer, it still remains the second leading cause of death in the United States and poses a major health and economic burden worldwide. While traditional treatments like surgery, chemotherapy, radiation therapy, and hormone therapy have been successful and have decreased cancer mortality, cancer incidence in all sites continues to rise. Consequently, there is an immediate need to find new therapeutics for the treatment of cancer. In recent years, and with the continuing push towards personalized medicine, cancer biomarkers have become crucial to detect, treat, and monitor ...


Microbiome Of Commercial Broilers Through Evisceration And Immersion Chilling, John A. Handley May 2018

Microbiome Of Commercial Broilers Through Evisceration And Immersion Chilling, John A. Handley

Theses and Dissertations

The United States poultry industry generated 38.6 billion pounds (17,500 metric ton) of meat in 2014 which averaged to 121 pounds (55 kg) per individual of the U.S that same year. Of that meat generated by the poultry industry, an estimated 1 million cases of Salmonellosis will occur. Out of the 1 million cases approximately 40, 000 to 50,000 will be confirmed cases by the CDC. Recently, the USDA has requested changes in the inspection process and are currently allowing processors more freedom to utilize innovation to drive the increase in safer and more desirable foods ...


Characterizing How Glycerol Monolaurate (Gml) Affects Human T Cell Signaling And Function, Michael Sining Zhang May 2018

Characterizing How Glycerol Monolaurate (Gml) Affects Human T Cell Signaling And Function, Michael Sining Zhang

Theses and Dissertations

The T cell receptor (TCR) activation induced signaling cascade is a major driver of T cell effector responses such as cytokine production and actin cytoskeletal rearrangement. Characterizing chemical modulators of this pathway has the benefits of both revealing basic science knowledge about these signaling processes and providing foundation for development of novel therapeutics.

Glycerol Monolaurate (GML) is a naturally occurring fatty acid monoester that is found as a monoglyceride in human breast milk and coconut oil. It is widely utilized in food, cosmetics, and homeopathic supplements. GML is a potent antimicrobial agent that targets a wide range of bacteria, fungi ...


Integrating Viral Vectors As A Gene Therapy Approach For Cystic Fibrosis, Ashley L. Cooney May 2018

Integrating Viral Vectors As A Gene Therapy Approach For Cystic Fibrosis, Ashley L. Cooney

Theses and Dissertations

Cystic fibrosis (CF) is the most common autosomal recessive genetic disease in Caucasian populations. CF affects multiple organ systems including pancreas, liver, intestines, sweat glands, and male reproductive organs, however the leading cause of morbidity and mortality in CF patients is chronic lung disease. CF is caused by a mutant cystic fibrosis transmembrane conductance regulator (CFTR) gene which leads to chloride (Cl-) and bicarbonate (HCO3-) anion dysregulation at the airway surface. Without adequate anion exchange, thick, viscous mucus accumulates at the airway surface allowing bacterial colonization to occur. Complementing CFTR in the appropriate airway cells restores the anion channel activity ...


The Role Of The Anti-Σ Factor Rsiv In Stress Response In Clostridium Difficile And Bacillus Subtilis, Ana N. Castro May 2018

The Role Of The Anti-Σ Factor Rsiv In Stress Response In Clostridium Difficile And Bacillus Subtilis, Ana N. Castro

Theses and Dissertations

Extra Cytoplasmic Function (ECF) σ factors are a diverse family of alternative σ factors that allow bacteria to sense and respond to changes in the environment. σV is an ECF σ factor found primarily in low GC Gram-positive bacteria and is required for lysozyme resistance in several opportunistic pathogens. In the absence of lysozyme, σV is inhibited by the anti-σ factor RsiV. In response to lysozyme, RsiV is degraded via the process of Regulated Intramembrane Proteolysis (RIP). RIP is initiated by cleavage of RsiV at site-1 which allows the intramembrane protease RasP to cleave RsiV within the transmembrane domain at ...


Hospital And Meat Associated Staphylococcus Aureus And Their Biofilm Characteristics, Trevor Michael Wienclaw Apr 2018

Hospital And Meat Associated Staphylococcus Aureus And Their Biofilm Characteristics, Trevor Michael Wienclaw

Theses and Dissertations

Biofilm phenotypes were studied in 32 Staphylococcus aureus strains isolated from store-bought meats and 22 from diseased patients in hospitals. Of the meat-associated strains, 21 were methicillin-resistant Staphylococcus aureus (MRSA) and 11 were methicillin-susceptible Staphylococcus aureus (MSSA). The hospital-associated strains included 15 MRSAs and 7 MSSAs. We studied the robustness and composition of the biofilms produced by these strains. We found that on average hospital-associated strains form more robust biofilms than meat associated strains. The model often used to describe S. aureus biofilm composition includes two biofilm types defined by the presence or absence of polysaccharide intercellular adhesin (PIA), PIA-dependent ...


Characterization Of Silicon Ion Exposure On Deinococcus Radiodurans, Richard F. Daughtry Mar 2018

Characterization Of Silicon Ion Exposure On Deinococcus Radiodurans, Richard F. Daughtry

Theses and Dissertations

Deinococcus radiodurans is a robust bacterium that is known for its extraordinary resistance to ionizing radiation. In general, many of the investigations of this bacterium's resistance have revolved around low linear energy transfer radiation, such as gamma and electron radiation. This study explored Deinococcus radiodurans's ability to survive high linear energy transfer radiation, specifically proton and neutron radiation. Deinococcus radiodurans was dehydrated to reduce the effects of low linear energy transfer radiation. The bacteria were exposed to both neutron and proton radiation of varying amounts and rehydrated. The resulting colonies were counted and compared to colonies of non-irradiated ...


Characterization Of The Effects Of Heavy Charged Particle Exposure On The Radiation Resistant Bacterium Deinococcus Radiodurans, Todd A. Bryant Mar 2018

Characterization Of The Effects Of Heavy Charged Particle Exposure On The Radiation Resistant Bacterium Deinococcus Radiodurans, Todd A. Bryant

Theses and Dissertations

Although not an extremophile, Deinococcus radiodurans has proven to have exceptional resistance to ionizing radiation, specifically via gamma and X-ray photons. To date, no known experiments have bombarded the bacterium with charged particles larger than hydrogen. This study explored the effects on the organism’s ability to survive high linear energy transfer heavy-charged particle exposures of oxygen ions. It also investigated the effects of low linear energy transfer ultraviolet radiation on various mutants. Two uvrB mutants were created to ascertain the importance of the gene in single-strand break repair following gamma irradiation and two recF mutants were created to explore ...


Role Of Incompatibility Group 1 (Inci1) Plasmid-Encoded Factors On Salmonella Enterica Antimicrobial Resistance And Virulence, Pravin Raghunath Kaldhone Dec 2017

Role Of Incompatibility Group 1 (Inci1) Plasmid-Encoded Factors On Salmonella Enterica Antimicrobial Resistance And Virulence, Pravin Raghunath Kaldhone

Theses and Dissertations

Foodborne illnesses are a leading cause of infectious diseases in the world. Among enteric organisms Salmonella is a key pathogen. It’s high prevalence in poultry and other food-animal sources make it imperative to study. Salmonella has the ability to modify its genetic content with help of mobile genetic elements such as plasmids. Incompatibiltiy group 1 (IncI1) plasmids are commonly reported in Salmonella. This study evaluates role on IncI1 plasmids in antimicrobial resistance and virulence in Salmonella. Genetic determinants of resistance and virulence are noted among our IncI1-containing Salmonella isolates. These genetic elements are also transferable and reported to carry ...


Experimental Approaches To Understand And Control Salmonella Infection In Poultry, Yichao Yang Dec 2017

Experimental Approaches To Understand And Control Salmonella Infection In Poultry, Yichao Yang

Theses and Dissertations

Salmonella is a major foodborne pathogen around the world and chickens are the major reservoir to transmit Salmonella into the human food chain. For decreasing the infection of Salmonella, we developed six attenuated live vaccines based on Salmonella Enteritidis (SE) and Typhimurium (ST) for testing the cross-serovar and cross-serogroup protection from the challenge of Salmonella Heidelberg and Campylobacter jejuni. One of the constructed vaccine strain showed ability to protect against challenge from Salmonella Heidelberg. Even though some preventive approaches are able to decrease Salmonella colonization in the gastrointestinal tract of chickens or other farm animals, Salmonella transmission mechanisms remain unclear ...


New Insights Into Nsp15 Protein And Rna Elements During Mouse Hepatitis Virus Infection, Jeremiah Athmer Dec 2017

New Insights Into Nsp15 Protein And Rna Elements During Mouse Hepatitis Virus Infection, Jeremiah Athmer

Theses and Dissertations

The non-structural protein 15 (NSP15) locus in Lineage A β-coronaviruses has two important functions during replication. The encoded endoribonuclease is conserved among coronaviruses. The function of the nsp15 protein is still not fully understood, but recent evidence suggests it may be involved in both replication and inhibiting viral sensing of double stranded RNA. In Lineage A β-coronaviruses, the RNA locus contains an inserted packaging signal (P/S). The P/S is essential for selectively packaging viral genomic RNA. While the P/S is required for selective packaging, it is not required for nsp15 protein function or viral replication. Utilizing this ...


Influence Of Epstein-Barr Virus On Systemic Lupus Erythematosus Disease Development And The Role Of Depression On Disease Progression, Caleb Cornaby Dec 2017

Influence Of Epstein-Barr Virus On Systemic Lupus Erythematosus Disease Development And The Role Of Depression On Disease Progression, Caleb Cornaby

Theses and Dissertations

Systemic Lupus Erythematosus (SLE) is an autoimmune disease affecting 20 to 250 individuals per 100,000 worldwide. Symptomology includes dermatological manifestations such as discoid lesions, acute cutaneous rashes, and oral and nasal ulcers, along with musculoskeletal, pulmonary, and renal complications. Abnormal T and B lymphocyte function and apoptosis, immune complex clearance, complement function, and nucleosome processing are typical of disease pathophysiology. SLE is the result of both environmental and genetic factors, which together create the conditions leading to disease onset and progression. Of these environmental factors, Epstein-Barr virus (EBV) infection is known to cause the genesis of cross-reactive antibodies in ...


Acetobacter Fabarum Genes Influencing Drosophila Melanogaster Phenotypes, Kylie Makay White Dec 2017

Acetobacter Fabarum Genes Influencing Drosophila Melanogaster Phenotypes, Kylie Makay White

Theses and Dissertations

Research in our lab has predicted hundreds of bacterial genes that influence nine different traits in the fruit fly, Drosophila melanogaster. As a practical alternative to creating site-directed mutants for each of the predicted genes, we created an arrayed transposon insertion library using a strain of Acetobacter fabarum DsW_054 isolated from fruit flies. Creation of the Acetobacter fabarum DsW_054 gene knock-out library was done through random transposon insertion, combinatorial mapping and Illumina sequencing. Successful mapping of transposon insertion was achieved for 6418 mutants with hits within 63% of annotated genes within Acetobacter fabarum DsW_054. Insertion sites were verified in 40 ...


Phosphate Signaling Through Alternate Conformations Of The Pstscab Phosphate Transporter, Ramesh Krishna Vuppada Dec 2017

Phosphate Signaling Through Alternate Conformations Of The Pstscab Phosphate Transporter, Ramesh Krishna Vuppada

Theses and Dissertations

Phosphate is an essential compound for life. Escherichia coli employs a signal transduction pathway that controls the expression of genes that are required for the high-affinity acquisition of phosphate and the utilization of alternate sources of phosphorous. These genes are only expressed when environmental phosphate is limiting. The seven genes for this signaling pathway encode the two-component regulatory proteins PhoB and PhoR, as well as the high-affinity phosphate transporter PstSCAB and an auxiliary protein called PhoU. As the sensor kinase PhoR has no periplasmic sensory domain, the mechanism by which these cells sense environmental phosphate is not known. This paper ...


An Inducible Fluorescent Reporter System To Measure Lux Operon Promoter Activity, Nicole Thunes Aug 2017

An Inducible Fluorescent Reporter System To Measure Lux Operon Promoter Activity, Nicole Thunes

Theses and Dissertations

Bioluminescence is the enzymatic production of light by a living organism. Many species of marine bacteria produce light with varying degrees of brightness. The lux operon is responsible for bioluminescence and is well studied, however it is currently unknown why different species of bacteria display different brightness levels. A dual-plasmid system designed to mimic the quorum-sensing induction of the lux operon was created and successfully implemented in E. coli. This was accomplished through the use of an arabinose-inducible plasmid containing a luxR gene from Vibrio harveyi, and then using the resulting LuxR protein to activate the lux promoter in a ...


The Role Of Pilj And Its Structural Domains In The Localization And Function Of The Chp Chemosensory System In Pseudomonas Aeruginosa, Vibhuti Hemantkumar Jansari Aug 2017

The Role Of Pilj And Its Structural Domains In The Localization And Function Of The Chp Chemosensory System In Pseudomonas Aeruginosa, Vibhuti Hemantkumar Jansari

Theses and Dissertations

Bacteria detect environmental signals using membrane-bound methyl-accepting chemotaxis proteins (MCPs), which are part of a larger complex of chemosensory proteins. Pseudomonas aeruginosa has four functionally distinct chemosensory protein complexes. The Chp chemosensory system regulates type IV pili mediated twitching motility and intracellular levels of cAMP by modulating the activity of an adenylate cyclase, CyaB. The Chp system is also proposed to be involved in type IV pili mediated directional twitching motility towards phosphatidylethanolamine (PE). PilJ is the only MCP predicted to be associated with the Chp system. In this study we investigated different domains of PilJ in order to decipher ...