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Bioinformatic Analysis Of The Flathead Lake Monster Bacteriophage, Jake Plagenz Apr 2019

Bioinformatic Analysis Of The Flathead Lake Monster Bacteriophage, Jake Plagenz

Life and Environmental Sciences Undergraduate Theses

The Flathead Lake Monster (FLM) bacteriophage was noted to have an abnormally-long tail upon its discovery. Once its genome was sequenced, this research sought out to identify the 117 FLM gene products using the BLASTp sequence alignment algorithm. This resulted in the discovery of five genes that are considered to be novel to the FLM. A specific gene within the FLM genome called the tape measure gene (TMG) was further analyzed once it was identified based on homology with other phages. Previous literature has suggested that a longer TMG can manifest itself as a longer bacteriophage tail length. This observation ...


Climatic Factors Limiting The Distribution Of Dermacentor Andersoni In Montana’S Rocky Mountain Region, Kyle Griffith May 2017

Climatic Factors Limiting The Distribution Of Dermacentor Andersoni In Montana’S Rocky Mountain Region, Kyle Griffith

Life and Environmental Sciences Undergraduate Theses

The Rocky Mountain Wood tick, Dermacentor andersoni, serves as the primary vector for the Colorado tick fever virus in Montana’s Rocky Mountain region. This disease presents symptoms such as fever, headaches, and myalgia; and generally lasts for approximately three weeks. Factors affecting the distribution of D. andersoni in Montana are not well known, limiting our ability to locate areas endemic to the CTF virus. To determine if various climatic factors influenced the distribution of D. andersoni Montana’s Rocky Mountain region, tick samples from Western Montana were collected and relative tick abundance was tested for association with six different ...


Natural Vertical Transmission Of Wnv In Montana, Inderbir Bains Mar 2017

Natural Vertical Transmission Of Wnv In Montana, Inderbir Bains

Life and Environmental Sciences Undergraduate Theses

West Nile Virus (WNV) emerged and rapidly spread throughout the United States within several years. WNV is spread in Montana primarily by the mosquito vector Culex tarsalis. Horizontal transmission of WNV has been extensively studied, while little is known regarding vertical transmission. Previous research suggests vertical transmission is possible in controlled laboratory studies. This research attempts to document natural vertical transmission in Montana. Larvae were collected and analyzed for WNV via RTPCR. Results show no presence of natural vertical transmission. Culex larvae and adult male mosquito collection methods were insufficient, fluctuations in temperatures and variant rainfall were contributing factors. Future ...


Isolation And Characterization Of A Bacteriophage The Flathead Lake Monster, Ian Lorang Apr 2016

Isolation And Characterization Of A Bacteriophage The Flathead Lake Monster, Ian Lorang

Life and Environmental Sciences Undergraduate Theses

It has been suggested that bacteriophages are the most abundant entities on the planet. The goal of this study was to isolate and characterize a novel phage. Few phages have been isolated, which provides this study a good chance to isolate a novel phage from Northwest Montana where a phage has never been isolated. Using plaque techniques, restriction digest, and phage enzyme tool a phage was isolated and named the Flathead Lake Monster (FLM) and is a novel phage based on results from the study. FLM had abnormally small plaque diameters and an unusually long tail. Compared to literature on ...


Characterization Of A Novel Mycobacteriophage Via Sequence Analysis, Sarah Lapierre Apr 2015

Characterization Of A Novel Mycobacteriophage Via Sequence Analysis, Sarah Lapierre

Life and Environmental Sciences Undergraduate Theses

Bacteriophages provide a relatively new area of scientific research. Approximately 1031 viruses on earth are capable of infecting bacteria and thereafter disseminating. Despite their widespread and pervasive nature, bacteriophages have been relatively unstudied. Not only do they provide a new class of organism but they also present potential usefulness to humans, including within the medical field. Bacteriophages represent a new tool in medicine’s ever-developing fight against bacterial infections. Notably, bacteriophages may offer a new weapon to battle infections that have developed resistance to multiple antibiotics. The prospect of bacteriophage use against infectious processes will require significant research which begins ...


Isolation And Analysis Of The Novel Mycobacteriophage, Ccp1, Kevin Mcnamee Apr 2015

Isolation And Analysis Of The Novel Mycobacteriophage, Ccp1, Kevin Mcnamee

Life and Environmental Sciences Undergraduate Theses

Bacteriophages are the largest remaining body of undescribed biodiversity on the planet. The present study attempted to isolate and analyze the structure and genetics of a novel mycobacteriophage extracted from a soil sample. It was hypothesized that undescribed phages exist in the study area. A phage, named CCP1, was isolated from soil obtained in Helena, Montana, U.S.A. via serial plating of phage material taken from plaques formed in Mycobacterium smegmatis. Genetic and morphological analyses by restriction enzyme fragment length polymorphism (RFLP) analysis and transmission electron microscopy (TEM) respectively, suggest that CCP1 is a novel mycobacteriophage.


Rt-Pcr Survey Of Mosquitos In Montana, North Dakota, & South Dakota For The Presence Of Cache Valley Virus, Paige Esposito Apr 2013

Rt-Pcr Survey Of Mosquitos In Montana, North Dakota, & South Dakota For The Presence Of Cache Valley Virus, Paige Esposito

Life and Environmental Sciences Undergraduate Theses

In order to contribute to knowledge of Cache Valley Virus, a virus that causes ovine fetal malformations and stillbirths, this study conducted a survey of six mosquito species in three Northwestern states to determine the primary vector of the virus. Total RNA was extracted from mosquito samples and subjected to reverse transcription PCR to determine if viral RNA was present. Of the 369 samples tested, none tested positive for viral RNA. This outcome suggests either that the virus 1) is not present, 2) may be present at low levels or 3) may be carried by another insect. An increase in ...


The Development Of An Efficient Immuno-Slotblotting Technique To Quantify The Effects Of Protein Disulfide Isomerase On Prion Protein Misfolding, Spencer Johnson Apr 2012

The Development Of An Efficient Immuno-Slotblotting Technique To Quantify The Effects Of Protein Disulfide Isomerase On Prion Protein Misfolding, Spencer Johnson

Life and Environmental Sciences Undergraduate Theses

The objective of this project was to develop an efficient immuno-slotblotting technique that could be used as a quantitative assay in measuring the effects of Protein Disulfide Isomerase (PDI) on prion protein misfolding in Chronic Wasting Disease (CWD). Chronic Wasting Disease is a transmissible spongiform encephalopathy that is horizontally transmitted amongst families of cervids, which includes elk, deer, and moose. The infectious agent in CWD is a misfolded prion protein that comes into contact with other prion proteins, causing them to misfold. Previous observations have suggested that disulfide bond rearrangement may be an important step in the misfolding process. In ...


Use Of Mirna Inhibitors To Investigate Probiotic Regulation Of E-Cadherin Expression, Ashtin Jeney Apr 2012

Use Of Mirna Inhibitors To Investigate Probiotic Regulation Of E-Cadherin Expression, Ashtin Jeney

Life and Environmental Sciences Undergraduate Theses

Studies have repeatedly focused on the positive effects of probiotic bacteria on mammalian gastrointestinal health and have confirmed the ability of probiotics, like lactobacilli, to decisively influence the intestinal microbiota balance and the gut immune system. The four species of lactobacilli used in this study, L. fermentum, L. acidophilus, L. gasseri, and L. rhamnosus, are known to stimulate the integrity of the intestinal barrier by promoting the expression of the adherence junction protein E-cadherin. Another major influence on E-cadherin concentrations is the presence of the transcription repressor SNAIL. This study aims to further investigate the mechanisms by which these species ...


Plasmid-Mediated Transference Of Multiple-Antibiotic Resistance Between Escherichia Coli Isolates In The Western Montana Region, Karyn Beiber Apr 2009

Plasmid-Mediated Transference Of Multiple-Antibiotic Resistance Between Escherichia Coli Isolates In The Western Montana Region, Karyn Beiber

Life and Environmental Sciences Undergraduate Theses

This thesis investigates the multiple drug resistant (MDR) characteristic within enteric Escherichia coli (E. coli) isolates and their ability to transfer this phenotype via resistant-plasmid (R-plasmid) conjugation. E. coli were isolated from bovine fecal samples in the western Montana region. These isolates were subjected to antibiotic resistance testing, then sorted and stored based on general resistance to two broad-spectrum antibiotics, ampicillin and chlorotetracycline. All isolates underwent molecular differentiation using RAPD PCR, then plasmid extraction using a Qiagen Maxi kit. These results were compared to determine if genetically similar isolates, as determined by RAPD PCR, were more likely to contain a ...


Prevalent Multiple Antibiotic Resistance Phenotypes In Commensal Escherichia Coli Isolated From Bovine Feces Of The Rocky Mountain Region Of Montana, Jacqueline Schmidt Apr 2009

Prevalent Multiple Antibiotic Resistance Phenotypes In Commensal Escherichia Coli Isolated From Bovine Feces Of The Rocky Mountain Region Of Montana, Jacqueline Schmidt

Life and Environmental Sciences Undergraduate Theses

The phenomenon of antibiotic resistance has lead to heightened discussion on how these adaptations may have arisen with such universality. Why antibiotic resistance occurs in an unpredictable manner within bacterial populations is poorly understood. It has been observed that resistance to many respective antibiotics is seen within the same organism as part of one phenotype. Each class of antibiotic has a distinct mode of action suggesting that adaptation to one antibiotic would not necessary illicit resistance to another. This study evaluates and outlines the environmental contributions to these multiple antibiotic resistance phenotypes present in bovine of the Rocky Mountain region ...


The Effect Of Culture Medium Composition On The Localization Of Peroxisomal Membrane Proteins In Yeast, Maria Miller Apr 2009

The Effect Of Culture Medium Composition On The Localization Of Peroxisomal Membrane Proteins In Yeast, Maria Miller

Life and Environmental Sciences Undergraduate Theses

Formation and degradation of peroxisomes are not well understood, but their absence leads to serious consequences. In the study described in this thesis, 1 sought to (1) verify previous work indicating that switching from glucose-rich medium to lipid-rich medium induces peroxisome formation; (2) reveal that switching back from lipid-rich to glucose-rich medium induces peroxisome degradation; (3) test the hypothesis that Pex3p is a class II PMP that enters the peroxisome from the endoplasmic reticulum during de novo peroxisome formation; and (4) test the hypothesis that Pxalp is a class I PMP that enters the peroxisomal membrane from the cytosol. I ...


The Use Of Sm-Fret Spectroscopy To Determine Whether Cooperative Binding Is Involved In The Chaperone Function Of Hiv-1 Nucleocapsid Protein, Elizabeth Swanson Apr 2008

The Use Of Sm-Fret Spectroscopy To Determine Whether Cooperative Binding Is Involved In The Chaperone Function Of Hiv-1 Nucleocapsid Protein, Elizabeth Swanson

Life and Environmental Sciences Undergraduate Theses

Human immunodeficiency virus nucleocapsid protein (HIV-1 NC) is known to have both structural and nucleic acid chaperone functions in the replication cycle of the retrovirus. As a nucleic acid chaperone, NC protein interacts with TAR RNA and TAR DNA structures during the minus-strand transfer step of reverse transcription. The aim of this study is to use single molecule florescence resonance energy transfer (SM-FRET) spectroscopy to study biotin-immobilized TAR DNA hairpins at various concentrations of NC protein. The resulting data will subsequently be used to determine whether cooperative binding occurs between the NC protein and the TAR DNA hairpins. The results ...


Influence Of Sex, Age, And Antibiotic Soap Use On The Number And Antibiotic Resistance Of Bacteria, Anna Bramucci Apr 2007

Influence Of Sex, Age, And Antibiotic Soap Use On The Number And Antibiotic Resistance Of Bacteria, Anna Bramucci

Life and Environmental Sciences Undergraduate Theses

This research dealt with factors that may impact the number of enteric and fecal coliform bacteria and the antibiotic resistance of the bacteria present on the hands of children ages one to 15. The targeted bacteria were the subset enteric bacteria, which cause potentially lethal cases of diarrhea in the United States and worldwide. Further exacerbating the mortality rate associated with enteric bacterial infections is the degree of antibiotic resistance acquired by the bacteria. The sterile bag method was used to acquire bacteria from the hands of children; the bacteria were counted and tested for degrees of antibiotic resistance using ...


Effects Of Varying Concentrations Of Zinc On Bacterial Tolerance, Diversity, And Community Structure In Artificially Amended Soils, Joshua Sykes Apr 2005

Effects Of Varying Concentrations Of Zinc On Bacterial Tolerance, Diversity, And Community Structure In Artificially Amended Soils, Joshua Sykes

Life and Environmental Sciences Undergraduate Theses

Controlled experiments in laboratory settings examining the effect of zinc pollution on bacterial communities in soil often neglect metal exposure over a period of time. Increasing levels of soil-zinc have been shown to increase the Zn tolerance of bacterial communities in soil and decrease diversity and change community structure of bacterial populations. Since soils are a critical component of terrestrial systems, the soil must be able to recover from a pollution event in order to maintain the quality of natural and agricultural systems. This study analyzed bacterial tolerance and diversity in soils containing 0,12.5,25, and 50 mM ...


Long Term Effects Of Heavy Metal Contamination On Bacterial Communities In The Upper Clark Fork River Canyon, Marcus Simonich Apr 2005

Long Term Effects Of Heavy Metal Contamination On Bacterial Communities In The Upper Clark Fork River Canyon, Marcus Simonich

Life and Environmental Sciences Undergraduate Theses

Microorganisms and their activities drive most ecosystems and therefore must be assessed in order to study ecosystem stability. Heavy metal contamination is known to have adverse effects on soil bacteria. In this study, bacterial populations from polluted (PV and PB) and non-polluted (NV) soils were compared in order to better understand soil resiliency, ecosystem stability, and the long-term effects of heavy metals have on bacteria. Biological parameters of soil quality including diversity, structure, activity, and heavy metal tolerance were compared and assessed. The numbers of Zn and Cu tolerant bacteria were determined by spread plating diluted soil samples onto agar ...


The Effects Of Soil Copper Concentration On The Activity And Diversity Of Microbial Communities, Jessica Davis Apr 2005

The Effects Of Soil Copper Concentration On The Activity And Diversity Of Microbial Communities, Jessica Davis

Life and Environmental Sciences Undergraduate Theses

Bacteria are an essential component of soil ecosystems, assisting in nutrient cycling and degradation of organic materials that maintain a healthy environment for growth of plants and other microorganisms. Stable levels of bacterial activity and diversity are indicative of a fully functional environment. Contamination with heavy metals, including copper, has been shown to adversely affect stability of soil microcosms through reduction of bacterial activity and diversity. Therefore, research was conducted to measure the microbial activity in soils a year after contamination with 0, 12.5, 25, and 50 mM copper using arginine ammonification and dehydrogenase activity assays. Also, bacterial diversity ...


Water Quality Survey Of Nitrate Levels And Coliform/Coliphage Presence In The Helena Valley Compared With Helena City Water, Emma Swingle Apr 2005

Water Quality Survey Of Nitrate Levels And Coliform/Coliphage Presence In The Helena Valley Compared With Helena City Water, Emma Swingle

Life and Environmental Sciences Undergraduate Theses

The well water of the Helena Valley has shown several significant problems in recent years. High nitrate levels and/or the presence of coliform and coliphage have been identified in numerous wells. These problems are likely the result of a combination of faulty sewage systems and regional agriculture. To effectively monitor and evaluate problem wells, data were collected and then statistically compared from thirteen Helena Valley wells with nine city water samples. The highly monitored city water contained minimal nitrate concentrations and no microbial contamination while the Helena Valley samples showed great diversity in contamination levels. Additionally, no correlations were ...


The Effects Of Heavy Metal Contamination On Bacterial Activities And Species Richness In Soils From Montana’S Clark Fork River Valley, Erin Carlson Apr 2004

The Effects Of Heavy Metal Contamination On Bacterial Activities And Species Richness In Soils From Montana’S Clark Fork River Valley, Erin Carlson

Life and Environmental Sciences Undergraduate Theses

Bacteria play an essential role in nutrient cycling processes essential to the survival of plant and animal life. These processes may be adversely affected by the degradation of soil quality by heavy metal pollution. Heavy metal contamination is a significant problem in southwestern Montana’s Clark Fork River Valley due to the activities of the Anaconda Copper Mining Company during the years 1884-1980. This study focused on the effects of heavy metal contamination on bacterial activities, population density, and species richness. Using dehydrogenase activity, pH, ICP-AES analysis, plate counts, and ribosomal intergenic spacer analysis (RISA), I determined the following: (i ...


The Effects Of Zinc Contamination On Soil Bacterial Communities, Andrew Palmer Apr 2004

The Effects Of Zinc Contamination On Soil Bacterial Communities, Andrew Palmer

Life and Environmental Sciences Undergraduate Theses

Relatively little is known about the effects of soil metal contamination on bacteria in laboratory environments. Extensive field studies have been done on the long-term effects of metal contamination on soil communities while relatively few studies have been performed in controlled environments. Zinc contamination is known to have an adverse effect on soil microorganisms that are important to ecosystem stability. The ability of microorganisms to adapt after an initial contamination is important in natural environments that may become contaminated. In this study the proportion of Zn tolerant bacteria and the activity of soil microorganisms were tested 2 days, 32 days ...


Effects Of Cu On Soil Bacterial Number, Activity, And Community Structure As A Function Of Time, Janelle Bennett Apr 2004

Effects Of Cu On Soil Bacterial Number, Activity, And Community Structure As A Function Of Time, Janelle Bennett

Life and Environmental Sciences Undergraduate Theses

Microorganisms are a critical component in determining the availability of nutrients in soil. Diversity of the soil microflora provides stability, as a more diverse flora will cycle nutrients over a potentially more diverse set of environmental conditions Contamination of the soil by heavy metals has been shown to adversely affect soil microorganisms and cause a decrease in diversity and activity, affecting the production of plant available forms of nutrients and thus decreasing soil quality. This study measured the effects of Cu pollution on microbial activity, number, and diversity as a function of time since exposure to Cu. Microbial communities were ...


The Effect Of Heavy Metal Pollution On Copper Tolerance And Antibiotic Resistance Of Soil Bacterial Communities, Stephanie Dull Apr 2003

The Effect Of Heavy Metal Pollution On Copper Tolerance And Antibiotic Resistance Of Soil Bacterial Communities, Stephanie Dull

Life and Environmental Sciences Undergraduate Theses

The bacterial composition of soils is important in the study of ecosystem ecology. Soil bacteria are involved in nutrient cycling, soil nutrition, and degradation of organic matter, and provide a healthy environment for plants and other microorganisms. Contamination of the soil by heavy metals has been shown to have adverse effects on the bacterial communities living in soil. Heavy metals can cause shifts in pH, providing an unstable environment for many species ofbacteria. Also, it is quite common for the genes for resistance to heavy metals and for antibiotic resistance to be found together on a plasmid, therefore, conferring resistance ...


The Effect Of Heavy Metal Pollution On Copper Tolerance And Antibiotic Resistance Of Soil Bacterial Communities, Stephanie Dull Apr 2003

The Effect Of Heavy Metal Pollution On Copper Tolerance And Antibiotic Resistance Of Soil Bacterial Communities, Stephanie Dull

Life and Environmental Sciences Undergraduate Theses

The bacterial composition of soils is important in the study of ecosystem ecology. Soil bacteria are involved in nutrient cycling, soil nutrition, and degradation of organic matter, and provide a healthy environment for plants and other microorganisms. Contamination of the soil by heavy metals has been shown to have adverse effects on the bacterial communities living in soil. Heavy metals can cause shifts in pH, providing an unstable environment for many species of bacteria. Also, it is quite common for the genes for resistance to heavy metals and for antibiotic resistance to be found together on a plasmid, therefore, conferring ...


Five Extracellular Proteins Of Group A Streptococcus: Antigenicity And Gene Expression, Elisha Stenseth Apr 2002

Five Extracellular Proteins Of Group A Streptococcus: Antigenicity And Gene Expression, Elisha Stenseth

Life and Environmental Sciences Undergraduate Theses

Five genes that encode novel extracellular proteins were previously identified through the genomic analysis of four Group A Streptococcus strains (serotypes Ml, M3, M5, and M18) (Reid et al., 2001). Four of the five proteins have an LPXTG amino acid motif at the carboxyterminus, a motif that covalently links extracellular proteins to the cell surface in many gram-positive pathogens. All of the genes encode proteins with a secretion signal sequence at the aminoterminus. In vivo expression was demonstrated with Western blots using sera obtained from 80 patients with invasive infections, non-invasive skin infections, pharyngitis, and acute rheumatic fever. Real-time reverse ...


The Production And Characterization Of A Mutant Form Of Herpes Virus Entry Mediator (Hvem), Christopher Gourley Apr 2002

The Production And Characterization Of A Mutant Form Of Herpes Virus Entry Mediator (Hvem), Christopher Gourley

Life and Environmental Sciences Undergraduate Theses

Herpes Simplex Virus (HSV) entry is a multistep process involving viral glycoproteins and cellular receptors. Viral glycoproteins bind cell-surface glycosaminoglycans, but this binding is not sufficient for virus entry. A cellular coreceptor is also essential for virus entry. Herpes Virus Entry Mediator (HVEM), an integral membrane protein, is a co-receptor that can mediate HSV entry. Hybrid proteins composed of HVEM and CD40 have shown that only one specific region, Cysteine Rich Repeat Domain 1 (CRRD1), of HVEM is essential to mediate HSV entry. The purpose ofthis project was to identify amino acids within CRRD1 essential for HVEM-mediated HSV entry. Site-directed ...


Mutagenesis Of The Vp16 Protein Of The Herpes Simplex Virus, Benjamin Spiger Apr 2000

Mutagenesis Of The Vp16 Protein Of The Herpes Simplex Virus, Benjamin Spiger

Life and Environmental Sciences Undergraduate Theses

The herpes simplex virus (HSV-1) encodes a trans-activator protein VP16. VP16 activates the immediate early genes (IE) of the viral genome during lytic infection. VP16 has been heavily studied by mutational analysis as a model for transcriptional activation. The goal of this project was to examine two specific aspects of VP16. First, to compare the properties of a previously constructed insertion mutant, in1814, to other mutant strains constructed in our laboratory; this insertion was moved from strain 17 of HSV-1 into the KOS strain, the strain used in our lab. The second aspect of this examination is a mutational analysis ...


Separation Of Empetrum Nigrum Compounds And Their Anti-Microbial Effects On Common Bacterial Species, Amy Evans Apr 1999

Separation Of Empetrum Nigrum Compounds And Their Anti-Microbial Effects On Common Bacterial Species, Amy Evans

Life and Environmental Sciences Undergraduate Theses

Empetrum nigrum, crowberry, is an arctic shrub known to have anti-microbial activity against Mycobacterium species and nine fungal species. Several compounds have been identified from the berries and the branches of E. nigrum. This report examines the separation of compounds from E. nigrum leaves and branches and the effects crude extracts have on common laboratory bacterial species. Crude extracts of the plant were obtained by soaking plants in solvents of various polarities. The methanol extract was further separated using reverse phase flash column chromatography. Following a reaction to remove glucose from a compound, NMR spectra were used to characterize the ...


A Comparison Of Microbial Hydrocarbon Degraders Based On The Source Of The Contaminant In Alaska, Andrew Gilbert Apr 1999

A Comparison Of Microbial Hydrocarbon Degraders Based On The Source Of The Contaminant In Alaska, Andrew Gilbert

Life and Environmental Sciences Undergraduate Theses

The overall objective of this study was to compare microbial hydrocarbon degraders based on their source of contaminant. The research involved enrichment of a single bacterial isolate from each of five hydrocarbon-contaminated soils collected at different sites in Alaska. After the strains were isolated from each source, they were compared to one another using various diagnostic tests. Forty-seven binary biochemical tests were performed. Of these only eighteen tests were discriminatory. These discriminatory tests were used for further statistical analysis. SYSTAT 5.1 was used to perform numerical cluster analyses which produced dendrograms showing normalized percent disagreement between responses to the ...


In Vitro Comparison Of Clarithromycin, Clarithromycin Plus 14-Hydroxy Clarithromycin, And Azithromycin Against Haemophilus Influenzae, Amy Arnold Apr 1998

In Vitro Comparison Of Clarithromycin, Clarithromycin Plus 14-Hydroxy Clarithromycin, And Azithromycin Against Haemophilus Influenzae, Amy Arnold

Life and Environmental Sciences Undergraduate Theses

The antibiotic activities of clarithromycin, clarithromycin plus 14-hydroxy clarithromycin and azithromycin against Haemophilus influenzae were compared. This comparison was performed because both clarithromycin and azithromycin have proven to be effective against infections due to H. influenzae. H. influenzae is partially responsible for the prevalence of community-acquired pneumonia (CAP) in the United States. Currently, many infectious and non-infectious conditions can mimic CAP, thereby hindering diagnoses of the causative agents and the administration of the most effective treatments. The initial treatment of CAP is crucial in reducing the mortality and morbidity due to CAP, yet the difficulties in diagnosis and administration of ...


An Analysis Of Extrachromosomal Dna Isolated From Bartonella Henselae, Martin Janout Apr 1996

An Analysis Of Extrachromosomal Dna Isolated From Bartonella Henselae, Martin Janout

Life and Environmental Sciences Undergraduate Theses

An unknown sequence of DNA discovered in Bartonella henselae was studied in order to determine rts conformation, size, and origin. The DNA was isolated from B. henselae cells using a standard miniprep method, commonly used to isolate small extrachromosomal DNA from cells. This DNA was subjected to a series of experiments including digestion with restriction endonucleases, SI, and Mung Bean nucleases, and hybridization to B. henselae genomic DNA. in order to determine whether there are any sequences in common. DNA fragments produced by digestion were separated using standard gel as well as Pulse-Field Gel Electrophoresis, and viewed after being stained ...