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Genetics and Genomics

Veterinary Microbiology and Preventive Medicine Publications

Virulence

Publication Year

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Full-Text Articles in Life Sciences

Papa Gene Of Avian Pathogenic Escherichia Coli, Subhashinie Kariyawasam, Lisa K. Nolan Dec 2011

Papa Gene Of Avian Pathogenic Escherichia Coli, Subhashinie Kariyawasam, Lisa K. Nolan

Veterinary Microbiology and Preventive Medicine Publications

P fimbrial adhesins may be associated with the virulence of avian pathogenic Escherichia coli (APEC). However, most APECs are unable to express P fimbriae even when they are grown under conditions that favor P fimbrial expression. This failure can be explained by the complete absence of the pap operon or the presence of an incomplete pap operon in Pap-negative APEC strains. In the present study, we analyzed the pap operon, specifically thepapA gene that encodes the major fimbrial shaft, to better understand the pap gene cluster at the genetic level. First, by PCR, we examined a collection of 500 ...


Pap Mutant Of Avian Pathogenic Escherichia Coli O1, An O1:K1:H7 Strain, Is Attenuated In Vivo, Subhashinie Kariyawasam, Lisa K. Nolan Jun 2009

Pap Mutant Of Avian Pathogenic Escherichia Coli O1, An O1:K1:H7 Strain, Is Attenuated In Vivo, Subhashinie Kariyawasam, Lisa K. Nolan

Veterinary Microbiology and Preventive Medicine Publications

P or Pap fimbriae of avian pathogenic Escherichia coli (APEC) have previously been shown to be important in the pathogenesis of colibacillosis. However, no study has been done to ascertain the effects of deletion of the genes responsible for P fimbrial biogenesis on APEC'sin vitro and/or in vivo characteristics. In the present study, all 11 genes of the pap gene cluster were deleted from APEC O1, the recently sequenced APEC strain, and the wild-type strain was compared to the mutant (ECPAP1) for changes in certain phenotypic characteristics and virulence for chickens. Both APEC O1 and ECPAP1 demonstrated ...


Virulence Genotyping Of Salmonella Spp. With Multiplex Pcr, Jerod A. Skyberg, Catherine M. Logue, Lisa K. Nolan Mar 2006

Virulence Genotyping Of Salmonella Spp. With Multiplex Pcr, Jerod A. Skyberg, Catherine M. Logue, Lisa K. Nolan

Veterinary Microbiology and Preventive Medicine Publications

The purpose of this study was to develop a multiplex polymerase chain reaction (PCR) protocol useful in the virulence genotyping of Salmonella spp. with the idea that genotyping could augment current Salmonella characterization and typing methods. Seventeen genes associated with Salmonella invasion, fimbrial production, toxin production, iron transport, and intramacrophage survival were targeted by three PCR reactions. Most of these genes are required for full Salmonella virulence in a murine model, and many are also located onSalmonella pathogenicity islands (PAIs) and are associated with type III secretion systems (TTSSs). Once the success of procedures that used positive and negative ...