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Genetics and Genomics

Veterinary Microbiology and Preventive Medicine Publications

Pap operon

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Full-Text Articles in Life Sciences

Papa Gene Of Avian Pathogenic Escherichia Coli, Subhashinie Kariyawasam, Lisa K. Nolan Dec 2011

Papa Gene Of Avian Pathogenic Escherichia Coli, Subhashinie Kariyawasam, Lisa K. Nolan

Veterinary Microbiology and Preventive Medicine Publications

P fimbrial adhesins may be associated with the virulence of avian pathogenic Escherichia coli (APEC). However, most APECs are unable to express P fimbriae even when they are grown under conditions that favor P fimbrial expression. This failure can be explained by the complete absence of the pap operon or the presence of an incomplete pap operon in Pap-negative APEC strains. In the present study, we analyzed the pap operon, specifically thepapA gene that encodes the major fimbrial shaft, to better understand the pap gene cluster at the genetic level. First, by PCR, we examined a collection of 500 ...


Occurrence Of Pathogenicity Island Iapec-O1 Genes Among Escherichia Coli Implicated In Avian Colibacillosis, Subhashinie Kariyawasam, Timothy J. Johnson, Chitrita Debroy, Lisa K. Nolan Sep 2006

Occurrence Of Pathogenicity Island Iapec-O1 Genes Among Escherichia Coli Implicated In Avian Colibacillosis, Subhashinie Kariyawasam, Timothy J. Johnson, Chitrita Debroy, Lisa K. Nolan

Veterinary Microbiology and Preventive Medicine Publications

Colibacillosis caused by avian pathogenic Escherichia coli (APEC) is a leading cause of economic loss to the poultry industry worldwide. The ability of APEC to cause disease is determined by certain virulence markers, some of which are located on pathogenicity islands (PAIs). We recently described one such PAI in an APEC O1:K1 strain (APEC-O1). This PAI, termed PAI IAPEC-O1, carries the genes of the pap operon, a region similar to the tiainvasion determinant of enterotoxigenic E. coli; ireA, a gene that encodes an iron-responsive element; and a novel 1.5-kb region, ORF 54. Here, the occurrence of ...