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Full-Text Articles in Life Sciences

Age-Related Dystrophin–Glycoprotein Complex Structure And Function In The Rat Extensor Digitorum Longus And Soleus Muscle, Kevin M. Rice, Deborah L. Preston, David Neff, Michael L. Norton, Eric R. Blough Oct 2006

Age-Related Dystrophin–Glycoprotein Complex Structure And Function In The Rat Extensor Digitorum Longus And Soleus Muscle, Kevin M. Rice, Deborah L. Preston, David Neff, Michael L. Norton, Eric R. Blough

Eric Blough

This study tested the hypothesis that age-related changes in the dystrophin–glycoprotein complex (DGC) may precede age-associated alterations in muscle morphology and function. Compared to those in adult (6 month) rats, extensor digitorum longus (EDL) and soleus muscle mass was decreased in old (30 month) and very old (36 month) Fischer 344/NNiaHSD 3 Brown Norway/ BiNia rats. The amount of dystrophin, b-dystroglycan, and a-sarcoglycan increased with aging in the EDL and decreased with aging in the soleus. a-Dystroglycan levels were increased with aging in both muscles and displayed evidence of altered glycosylation. Immunostaining for the presence of antibody infiltration ...


Age-Related Dystrophin–Glycoprotein Complex Structure And Function In The Rat Extensor Digitorum Longus And Soleus Muscle, Kevin M. Rice, Deborah L. Preston, David Neff, Michael L. Norton, Eric R. Blough Oct 2006

Age-Related Dystrophin–Glycoprotein Complex Structure And Function In The Rat Extensor Digitorum Longus And Soleus Muscle, Kevin M. Rice, Deborah L. Preston, David Neff, Michael L. Norton, Eric R. Blough

Kevin M Rice

This study tested the hypothesis that age-related changes in the dystrophin–glycoprotein complex (DGC) may precede age-associated alterations in muscle morphology and function. Compared to those in adult (6 month) rats, extensor digitorum longus (EDL) and soleus muscle mass was decreased in old (30 month) and very old (36 month) Fischer 344/NNiaHSD 3 Brown Norway/ BiNia rats. The amount of dystrophin, b-dystroglycan, and a-sarcoglycan increased with aging in the EDL and decreased with aging in the soleus. a-Dystroglycan levels were increased with aging in both muscles and displayed evidence of altered glycosylation. Immunostaining for the presence of antibody infiltration ...


Age-Related Dystrophin–Glycoprotein Complex Structure And Function In The Rat Extensor Digitorum Longus And Soleus Muscle, Kevin M. Rice, Deborah L. Preston, David Neff, Michael L. Norton, Eric R. Blough Oct 2006

Age-Related Dystrophin–Glycoprotein Complex Structure And Function In The Rat Extensor Digitorum Longus And Soleus Muscle, Kevin M. Rice, Deborah L. Preston, David Neff, Michael L. Norton, Eric R. Blough

Deborah L Preston

This study tested the hypothesis that age-related changes in the dystrophin–glycoprotein complex (DGC) may precede age-associated alterations in muscle morphology and function. Compared to those in adult (6 month) rats, extensor digitorum longus (EDL) and soleus muscle mass was decreased in old (30 month) and very old (36 month) Fischer 344/NNiaHSD 3 Brown Norway/ BiNia rats. The amount of dystrophin, b-dystroglycan, and a-sarcoglycan increased with aging in the EDL and decreased with aging in the soleus. a-Dystroglycan levels were increased with aging in both muscles and displayed evidence of altered glycosylation. Immunostaining for the presence of antibody infiltration ...


Dihydropyridine Receptors And Type 1 Ryanodine Receptors Constitute The Molecular Machinery For Voltage-Induced Ca2+ Release In Nerve Terminals, Valerie De Crescenzo, Kevin E. Fogarty, Ronghua Zhuge, Richard A. Tuft, Lawrence M. Lifshitz, Jeffrey Carmichael, Karl D. Bellve, Stephen P. Baker, Spyros Zissimopoulos, F. Anthony Lai, Jose R. Lemos, John V. Walsh Jr. Jul 2006

Dihydropyridine Receptors And Type 1 Ryanodine Receptors Constitute The Molecular Machinery For Voltage-Induced Ca2+ Release In Nerve Terminals, Valerie De Crescenzo, Kevin E. Fogarty, Ronghua Zhuge, Richard A. Tuft, Lawrence M. Lifshitz, Jeffrey Carmichael, Karl D. Bellve, Stephen P. Baker, Spyros Zissimopoulos, F. Anthony Lai, Jose R. Lemos, John V. Walsh Jr.

Information Technology Publications

Ca2+ stores were studied in a preparation of freshly dissociated terminals from hypothalamic magnocellular neurons. Depolarization from a holding level of -80 mV in the absence of extracellular Ca2+ elicited Ca2+ release from intraterminal stores, a ryanodine-sensitive process designated as voltage-induced Ca2+ release (VICaR). The release took one of two forms: an increase in the frequency but not the quantal size of Ca2+ syntillas, which are brief, focal Ca2+ transients, or an increase in global [Ca2+]. The present study provides evidence that the sensors of membrane potential for VICaR are dihydropyridine receptors (DHPRs). First, over the range of -80 to ...


Molecular Analysis Of The Effects Of Atrazine On Xenopus Laevis Frogs, Ronald Edward Celestine Jul 2006

Molecular Analysis Of The Effects Of Atrazine On Xenopus Laevis Frogs, Ronald Edward Celestine

Dissertations

Atrazine, the most commonly used herbicide in the U.S., has been implicated as one of the factors that may be causing the worldwide decline of amphibians. First, I examined effects of atrazine on laboratory raised Xenopus laevis tadpoles, treated from 5 days post fertilization to NF stage 65/66 with a concentrationof 25 ppb of atrazine. This study showed no significant difference in body weight, length and metamorphic rate.

Second, I used DNA microarrays to examine mRNA expression levels of genes in atrazine treated and control Xenopus laevis tadpoles. I found significant changes in gene expression levels of 18 ...


Stretch Increases Alveolar Epithelial Permeability To Uncharged Micromolecules, Kenneth Joseph Cavanaugh, Taylor Sitarik Cohen, Susan S. Margulies Apr 2006

Stretch Increases Alveolar Epithelial Permeability To Uncharged Micromolecules, Kenneth Joseph Cavanaugh, Taylor Sitarik Cohen, Susan S. Margulies

Departmental Papers (BE)

We measured stretch-induced changes in transepithelial permeability in vitro to uncharged tracers 1.5–5.5 Å in radius to identify a critical stretch threshold associated with failure of the alveolar epithelial transport barrier. Cultured alveolar epithelial cells were subjected to a uniform cyclic (0.25 Hz) biaxial 12, 25, or 37% change in surface area (ΔSA) for 1 h. Additional cells served as unstretched controls. Only 37% ΔSA (100% total lung capacity) produced a significant increase in transepithelial tracer permeability, with the largest increases for bigger tracers. Using the permeability data, we modeled the epithelial permeability in each group ...


Characterization Of 35 Microsatellite Loci In The Pacific Lion-Paw Scallop (Nodipecten Subnodosus) And Their Cross-Species Amplification In Four Other Scallops Of The Pectinidae Family, Ana M. Ibarra, Jessica Lynn Petersen, Thomas R. Famula, Bernie May Jan 2006

Characterization Of 35 Microsatellite Loci In The Pacific Lion-Paw Scallop (Nodipecten Subnodosus) And Their Cross-Species Amplification In Four Other Scallops Of The Pectinidae Family, Ana M. Ibarra, Jessica Lynn Petersen, Thomas R. Famula, Bernie May

Faculty Papers and Publications in Animal Science

Four microsatellite-enriched DNA libraries yielded 35 microsatellite loci from 100 primer pairs designed for Pacific lion-paw scallop, Nodipecten subnodosus. The number of alleles ranged from four to 28. Three of the 35 loci were not in Hardy–Weinberg equilibrium and linkage disequilibrium was found for one pair of loci. These microsatellites will be used to analyze the population structure of the species in Mexico’s Baja Peninsula to propose management strategies for scallop aquaculture development. Twenty-six primer pairs cross-amplified in Nodipecten nodosus, whereas none (Argopecten ventricosus) or few cross-amplified in the Argopecten species.