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Full-Text Articles in Life Sciences

A Critical Analysis Of Powell’S Results On The Interdivision Time Distribution, Vincent Quedeville, Jérôme Morchain, Philippe Villedieu, Rodney O. Fox Jun 2019

A Critical Analysis Of Powell’S Results On The Interdivision Time Distribution, Vincent Quedeville, Jérôme Morchain, Philippe Villedieu, Rodney O. Fox

Rodney O. Fox

The cell-age and interdivision-time probability density functions (PDFs) have been extensively investigated since the 1940s due to their fundamental role in cell growth. The pioneering work of Powell established the first relationship between the interdivision-time and cell-age PDFs. In the literature, two definitions for the interdivision-time PDF have been proposed. One stands for the age-at-rupture PDF and is experimentally observable, whereas the other is the probability density that a cell divides at a certain age and is unobservable. From Powell’s results pertaining to the unobservable interdivision-time PDF, Painter and Marr derived an inequality that is true but is incorrectly ...


Insulin Signal Transduction Mediates Ethanol-Induced Feeding Dysfunction In A Fly Model Of Fetal Alcohol Spectrum Disorder, Manae Matsubara Jun 2019

Insulin Signal Transduction Mediates Ethanol-Induced Feeding Dysfunction In A Fly Model Of Fetal Alcohol Spectrum Disorder, Manae Matsubara

McNair Research Journal SJSU

Fetal alcohol spectrum disorder (FASD) is the leading cause of congenital intellectual disabilities in the Western World, with a worldwide prevalence of 2-11% of all births. FASD is preventable but recent epidemiological studies suggest that public awareness campaigns have reached the limits of their effectiveness. Therefore, research is shifting from prevention to treatment and mitigation of symptoms. No biological treatments for FASD exist, due in part to the fact the cellular mechanisms of alcohol toxicity are not well-understood. Developmental alcohol exposure (DAE) causes a variety of deleterious effects in both vertebrates and invertebrates, including increased mortality, slow growth, learning and ...


A Critical Analysis Of Powell’S Results On The Interdivision Time Distribution, Vincent Quedeville, Jérôme Morchain, Philippe Villedieu, Rodney O. Fox Jun 2019

A Critical Analysis Of Powell’S Results On The Interdivision Time Distribution, Vincent Quedeville, Jérôme Morchain, Philippe Villedieu, Rodney O. Fox

Chemical and Biological Engineering Publications

The cell-age and interdivision-time probability density functions (PDFs) have been extensively investigated since the 1940s due to their fundamental role in cell growth. The pioneering work of Powell established the first relationship between the interdivision-time and cell-age PDFs. In the literature, two definitions for the interdivision-time PDF have been proposed. One stands for the age-at-rupture PDF and is experimentally observable, whereas the other is the probability density that a cell divides at a certain age and is unobservable. From Powell’s results pertaining to the unobservable interdivision-time PDF, Painter and Marr derived an inequality that is true but is incorrectly ...


Validation Experiment: Wild Type, Probability, Barresi Lab, Smith College Jun 2019

Validation Experiment: Wild Type, Probability, Barresi Lab, Smith College

Validation Experiment

No abstract provided.


Validation Experiment: Wild Type, Psi, Barresi Lab, Smith College Jun 2019

Validation Experiment: Wild Type, Psi, Barresi Lab, Smith College

Validation Experiment

No abstract provided.


Validation Experiment: Wild Type, Raw, At, Barresi Lab, Smith College Jun 2019

Validation Experiment: Wild Type, Raw, At, Barresi Lab, Smith College

Validation Experiment

No abstract provided.


Validation Experiment: Wild Type, Raw, Gfap, Barresi Lab, Smith College Jun 2019

Validation Experiment: Wild Type, Raw, Gfap, Barresi Lab, Smith College

Validation Experiment

No abstract provided.


Validation Experiment: You-Too, Probability, Barresi Lab, Smith College Jun 2019

Validation Experiment: You-Too, Probability, Barresi Lab, Smith College

Validation Experiment

No abstract provided.


Validation Experiment: You-Too, Psi, Barresi Lab, Smith College Jun 2019

Validation Experiment: You-Too, Psi, Barresi Lab, Smith College

Validation Experiment

No abstract provided.


Validation Experiment: You-Too, Raw, Barresi Lab, Smith College Jun 2019

Validation Experiment: You-Too, Raw, Barresi Lab, Smith College

Validation Experiment

No abstract provided.


Slit1a Rescue: Role Of Slit1a, Wt, Barresi Lab, Smith College Jun 2019

Slit1a Rescue: Role Of Slit1a, Wt, Barresi Lab, Smith College

Role of slit1a

No abstract provided.


Slit1a Rescue: Role Of Slit1a, Hss1a, Barresi Lab, Smith College Jun 2019

Slit1a Rescue: Role Of Slit1a, Hss1a, Barresi Lab, Smith College

Role of slit1a

No abstract provided.


Slit1a Rescue: Role Of Slit1a, Hss1ayot, Barresi Lab, Smith College Jun 2019

Slit1a Rescue: Role Of Slit1a, Hss1ayot, Barresi Lab, Smith College

Role of slit1a

No abstract provided.


Slit1a Rescue: Role Of Slit1a, Non-Hss1ayot, Barresi Lab, Smith College Jun 2019

Slit1a Rescue: Role Of Slit1a, Non-Hss1ayot, Barresi Lab, Smith College

Role of slit1a

No abstract provided.


Glial Bridge Development: 22 Hpf, Barresi Lab, Smith College Jun 2019

Glial Bridge Development: 22 Hpf, Barresi Lab, Smith College

Glial Bridge

No abstract provided.


Glial Bridge Development: 24 Hpf, Barresi Lab, Smith College Jun 2019

Glial Bridge Development: 24 Hpf, Barresi Lab, Smith College

Glial Bridge

No abstract provided.


Glial Bridge Development: 26 Hpf, Barresi Lab, Smith College Jun 2019

Glial Bridge Development: 26 Hpf, Barresi Lab, Smith College

Glial Bridge

No abstract provided.


Glial Bridge Development: 28 Hpf, Barresi Lab, Smith College Jun 2019

Glial Bridge Development: 28 Hpf, Barresi Lab, Smith College

Glial Bridge

No abstract provided.


Glial Bridge Development: 30 Hpf, Barresi Lab, Smith College Jun 2019

Glial Bridge Development: 30 Hpf, Barresi Lab, Smith College

Glial Bridge

No abstract provided.


Examining Lateral Line Development Through Cxcl14 Modulation Of Cxcl12-Cxcr4 Mediated Gene Expression In Danio Rerio, Ariana Calderon-Zavala May 2019

Examining Lateral Line Development Through Cxcl14 Modulation Of Cxcl12-Cxcr4 Mediated Gene Expression In Danio Rerio, Ariana Calderon-Zavala

Lawrence University Honors Projects

The lateral line is a mechanosensory system used by fish to sense the movement of water. It is evolutionarily related to the inner-ear in humans. For both organisms, the binding of the CXCL12 (SDF-1 ligand) to the CXCR4 receptor induces conformational changes needed to activate signal transduction. This signaling results in numerous cellular responses such as cell fate, chemotaxis, and gene transcription. Interestingly, researchers have found that another signaling molecule, CXCL14, can also bind to the CXCR4 receptor with high affinity (Tanegashima et al., 2013). As a result, we hypothesize that CXCL14 modulates CXCL12-mediated chemotaxis, presumably acting as an allosteric ...


Differential Roles Of Mammalian Target Of Rapamycin Complexes 1 And 2 In Migration Of Prostate Cancer Cells, Smrruthi Vaidegi Venugopal May 2019

Differential Roles Of Mammalian Target Of Rapamycin Complexes 1 And 2 In Migration Of Prostate Cancer Cells, Smrruthi Vaidegi Venugopal

Electronic Theses & Dissertations Collection for Atlanta University & Clark Atlanta University

In this study, we investigated differential activation and the role of two mTOR complexes in cell migration of prostate cancer cells. Specific knock-down of endogenous RAPTOR and RICTOR by siRNA resulted in decreased cell migration in LNCaP, DU145, and PC3 cells indicating that both mTORC1 and mTORC2 are required for cell migration. EGF treatment induced the activation of both mTORC1 and mTORC2 as determined by complex-specific phosphorylation of mTOR protein. Specific knock-down or inhibition of Rac1 activity in PC3 cells blocked EGF-induced activation of mTORC2, but had no effect on mTORC1 activation. Furthermore, the over-expression of constitutively active Rac1 (Rac1Q61L ...


Extraction, Purification And Evaluation Of Prmt5-Inhibitory Phytochemical Compounds For The Treatment Of Prostate Adenocarcinoma, Oliver H. Richmond Iii May 2019

Extraction, Purification And Evaluation Of Prmt5-Inhibitory Phytochemical Compounds For The Treatment Of Prostate Adenocarcinoma, Oliver H. Richmond Iii

Electronic Theses & Dissertations Collection for Atlanta University & Clark Atlanta University

The development and advancement of prostate cancer is supported by a plethora of genetic and proteomic abnormalities, including events of post-translational modifications. The protein arginine methyltransferase 5 (PRMT5) enzyme regulates epigenetic events of histone modifications and protein post-translational modifications within protein signaling pathways. PRMT5 functions by catalyzing the symmetric dimethylation of terminal arginine residues on target protein substrates. Under abnormal conditions of overexpression and upregulation, PRMT5 methyltransferase activity constitutively drives the growth and proliferation of dysregulated cells. Overexpression or upregulation of PRMT5 correlates with disease progression as observed among numerous cancer types, including breast, colorectal, leukemia, lung, melanoma and prostate ...


Role Of High Mobility Group A2 (Hmga2) In Prostate Cancer, Ohuod Hawsawi May 2019

Role Of High Mobility Group A2 (Hmga2) In Prostate Cancer, Ohuod Hawsawi

Electronic Theses & Dissertations Collection for Atlanta University & Clark Atlanta University

High mobility group A2 (HMGA2) is a non-histone protein highly expressed during the development but is low or absent in most adult tissues. Epithelial-mesenchymal transition (EMT) plays a critical role in prostate cancer progression and metastasis. HMGA2 has been shown to promote EMT in separate studies. Interestingly, wild-type HMGA2 and truncated (lacking the 3’UTR) HMGA2 isoforms are overexpressed in many cancers. However, there are no studies on the role of each isoform in prostate cancer progression. We hypothesized that wild-type and truncated HMGA2 promotes prostate cancer progression by different mechanisms. We analyzed the expression of HMGA2 in the prostate ...


Loss Of Id4 Promotes Stemness In Prostate Cancer Cells, Dhanushka Hewabostanthirige May 2019

Loss Of Id4 Promotes Stemness In Prostate Cancer Cells, Dhanushka Hewabostanthirige

Electronic Theses & Dissertations Collection for Atlanta University & Clark Atlanta University

Inhibitor of differentiation 4 (ID4), a member of the helix-loop-helix family of transcriptional regulators has emerged as a tumor suppressor in prostate cancer (PCa). Recent studies have shown that Id4 is highly expressed in the normal prostate and decreases in prostate cancer due to epigenetic silencing. Id4 knockdown in androgen sensitive LNCaP cells has been shown to lead to castration resistant prostate cancer (CRPC) in vitro and in vivo. Id4-/- mice leads to underdeveloped prostate with PIN like lesions without the loss of Androgen Receptor (AR) expression. In this study we demonstrate that the loss of ID4 expression in PCa ...


Establishment Of Crispr/Cas-9 Aided Knockout Of The Zic2 Gene In The African-American Prostate Cancer Cell Line E006aa-Pr, Janelle Moore May 2019

Establishment Of Crispr/Cas-9 Aided Knockout Of The Zic2 Gene In The African-American Prostate Cancer Cell Line E006aa-Pr, Janelle Moore

Electronic Theses & Dissertations Collection for Atlanta University & Clark Atlanta University

The largest U.S. cancer health disparity exists in prostate cancer, with African American men having the highest incidence and mortality rates. The present study evaluated the effects of ZIC2 and the underlying mechanisms in the E006 parental African-American cell line that produces tumors at accelerated growth rates because of the increase of ZIC2 genes in African-American males. We analyzed the experimental research that the overexpression of ZIC2 contributes to progression of prostate cancer. E006AA cells with overexpressed or suppressed ZIC2 were analyzed to determine phenotypic differences, PCR, cell proliferation and immunoblot assays. The expression levels of ZIC2 were analyzed ...


Reversal Of P-Glycoprotein And Breast Cancer Resistance Protein Mediated Multidrug Resistance In Vitro Using In Silico Identified Novel Compounds, Amila Nanayakkara May 2019

Reversal Of P-Glycoprotein And Breast Cancer Resistance Protein Mediated Multidrug Resistance In Vitro Using In Silico Identified Novel Compounds, Amila Nanayakkara

Biological Sciences Theses and Dissertations

Multidrug resistance (MDR) is a major cause of chemotherapy failure. Overexpression of ATP-binding cassette (ABC) transporters, P-glycoprotein (P-gp) and breast cancer resistance protein (BCRP) are two well-studied drug transporters which are associated with MDR. These two transporters also act as a major functional unit of the blood brain barrier to protect the brain from xenobiotics and toxins. Lack of clinically approved P-gp and BCRP inhibitors renders chemotherapy treatments of many MDR cancers ineffective and obstructs drug uptake into the brain.

Using computational methods, we have identified new compounds that inhibit P-gp (Brewer et al., Mol. Pharmacol. 2014). Several of these ...


Reversal Of P-Glycoprotein And Breast Cancer Resistance Protein Mediated Multidrug Resistance In Vitro Using In Silico Identified Novel Compounds, Amila Nanayakkara May 2019

Reversal Of P-Glycoprotein And Breast Cancer Resistance Protein Mediated Multidrug Resistance In Vitro Using In Silico Identified Novel Compounds, Amila Nanayakkara

Biological Sciences Theses and Dissertations

Multidrug resistance (MDR) is a major cause of chemotherapy failure. Overexpression of ATP-binding cassette (ABC) transporters, P-glycoprotein (P-gp) and breast cancer resistance protein (BCRP) are two well-studied drug transporters which are associated with MDR. These two transporters also act as a major functional unit of the blood brain barrier to protect the brain from xenobiotics and toxins. Lack of clinically approved P-gp and BCRP inhibitors renders chemotherapy treatments of many MDR cancers ineffective and obstructs drug uptake into the brain.

Using computational methods, we have identified new compounds that inhibit P-gp (Brewer et al., Mol. Pharmacol. 2014). Several of these ...


Network Analyses Of Glomerular Capillaries, Jason Cory Brunson, Justin Sardi, Mark Terasaki May 2019

Network Analyses Of Glomerular Capillaries, Jason Cory Brunson, Justin Sardi, Mark Terasaki

Biology and Medicine Through Mathematics Conference

No abstract provided.


Effects Of Herbicides On Zebrafish Embryo Development And Viability, Kayla Ray King May 2019

Effects Of Herbicides On Zebrafish Embryo Development And Viability, Kayla Ray King

MSU Graduate Theses

Environmental contaminants are chemicals of anthropogenic origin that are found in water, soil, and air, and are harmful to a wide variety of organisms (ORD US EPA, 2018-a). One common group of contaminants are herbicides. Though herbicides are used to control unwanted vegetation in agriculture, aquatic organisms and humans may be exposed to these herbicides through run off into streams and rivers, by drinking contaminated water, by consuming treated crops, by direct exposure, or through bioaccumulation. Thus the effect of these herbicides on animals needs further investigation. In this study, I sought to determine whether six different herbicides, which have ...


Towards A Better Understanding Of Temporomandibular Disorder, Jessica R. Cox May 2019

Towards A Better Understanding Of Temporomandibular Disorder, Jessica R. Cox

MSU Graduate Theses

Results from the OPPERA study provided evidence that risk factors such as neck muscle tension, prolonged jaw opening, and female gender increase the likelihood of developing temporomandibular joint disorders (TMJD), which are prevalent, debilitating orofacial pain conditions. Peripheral and central sensitization, which mediate a lowering of the stimulus required for pain signaling, are implicated in the underlying pathology of chronic TMJD. The goal of my study was to investigate cellular changes in the expression of proteins associated with the development of central sensitization. Female Sprague-Dawley rats were injected with complete Freund’s adjuvant in the upper trapezius muscles to promote ...