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Arts and Humanities Commons

Open Access. Powered by Scholars. Published by Universities.®

2005

Medicine and Health Sciences

University of Massachusetts Medical School

Women’s Health Research Faculty Publications

Animals; B-Lymphocytes; Cytidine Deaminase; DNA; DNA Glycosylases; Gene Expression Regulation; Gene Rearrangement, B-Lymphocyte; Immunoglobulin Constant Regions; Immunoglobulin mu-Chains; Mice; Mice, Mutant Strains; Recombination, Genetic; Somatic Hypermutation, Immunoglobulin; Spleen; Uracil-DNA Glycosidase

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Inducible Dna Breaks In Ig S Regions Are Dependent On Aid And Ung, Carol E. Schrader, Erin K. Linehan, Sofia N. Mochegova, Robert T. Woodland, Janet Stavnezer Aug 2005

Inducible Dna Breaks In Ig S Regions Are Dependent On Aid And Ung, Carol E. Schrader, Erin K. Linehan, Sofia N. Mochegova, Robert T. Woodland, Janet Stavnezer

Women’s Health Research Faculty Publications

Class switch recombination (CSR) occurs by an intrachromosomal deletion whereby the IgM constant region gene (Cmu) is replaced by a downstream constant region gene. This unique recombination event involves formation of double-strand breaks (DSBs) in immunoglobulin switch (S) regions, and requires activation-induced cytidine deaminase (AID), which converts cytosines to uracils. Repair of the uracils is proposed to lead to DNA breaks required for recombination. Uracil DNA glycosylase (UNG) is required for most CSR activity although its role is disputed. Here we use ligation-mediated PCR to detect DSBs in S regions in splenic B cells undergoing CSR. We find that the ...