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Medicine and Health Sciences

University of Massachusetts Medical School

Mismatch repair

Publication Year

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Full-Text Articles in Arts and Humanities

Mlh1 Can Function In Antibody Class Switch Recombination Independently Of Msh2, Carol E. Schrader, Joycelyn Vardo, Janet Stavnezer May 2003

Mlh1 Can Function In Antibody Class Switch Recombination Independently Of Msh2, Carol E. Schrader, Joycelyn Vardo, Janet Stavnezer

Women’s Health Research Faculty Publications

Mismatch repair proteins participate in antibody class switch recombination, although their roles are unknown. Previous nucleotide sequence analyses of switch recombination junctions indicated that the roles of Msh2 and the MutL homologues, Mlh1 and Pms2, differ. We now asked if Msh2 and Mlh1 function in the same pathway during switch recombination. Splenic B cells from mice deficient in both these proteins were induced to undergo switching in culture. The frequency of switching is reduced, similarly to that of B cells singly deficient in Msh2 or Mlh1. However, the nucleotide sequences of the Smu-Sgamma3 junctions resemble junctions from Mlh1- but not ...


Role For Mismatch Repair Proteins Msh2, Mlh1, And Pms2 In Immunoglobulin Class Switching Shown By Sequence Analysis Of Recombination Junctions, Carol E. Schrader, Joycelyn Vardo, Janet Stavnezer Feb 2002

Role For Mismatch Repair Proteins Msh2, Mlh1, And Pms2 In Immunoglobulin Class Switching Shown By Sequence Analysis Of Recombination Junctions, Carol E. Schrader, Joycelyn Vardo, Janet Stavnezer

Women’s Health Research Faculty Publications

B cells from mice deficient in mismatch repair (MMR) proteins show decreased ability to undergo class switch recombination in vitro and in vivo. The deficit is not accompanied by any reduction in cell viability or alterations in the cell cycle in B cells cultured in vitro. To assess the role of MMR in switching we examined the nucleotide sequences of Smicro-Sgamma3 recombination junctions in splenic B cells induced in culture to switch to IgG3. The data demonstrate clear differences in the sequences of switch junctions in wild-type B cells in comparison with Msh2-, Mlh1-, and Pms2-deficient B cells. Sequences of ...