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Medicine and Health Sciences

University of Massachusetts Medical School

Heavy chain

Publication Year

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Full-Text Articles in Arts and Humanities

Deletion Of The Nucleotide Excision Repair Gene Ercc1 Reduces Immunoglobulin Class Switching And Alters Mutations Near Switch Recombination Junctions, Carol E. Schrader, Joycelyn Vardo, Erin K. Linehan, Michael Z. Twarog, Laura J. Niedernhofer, Jan H. J. Hoeijmakers, Janet Stavnezer Aug 2004

Deletion Of The Nucleotide Excision Repair Gene Ercc1 Reduces Immunoglobulin Class Switching And Alters Mutations Near Switch Recombination Junctions, Carol E. Schrader, Joycelyn Vardo, Erin K. Linehan, Michael Z. Twarog, Laura J. Niedernhofer, Jan H. J. Hoeijmakers, Janet Stavnezer

Women’s Health Research Faculty Publications

The structure-specific endonuclease ERCC1-XPF is an essential component of the nucleotide excision DNA repair pathway. ERCC1-XPF nicks double-stranded DNA immediately adjacent to 3' single-strand regions. Substrates include DNA bubbles and flaps. Furthermore, ERCC1 interacts with Msh2, a mismatch repair (MMR) protein involved in class switch recombination (CSR). Therefore, ERCC1-XPF has abilities that might be useful for antibody CSR. We tested whether ERCC1 is involved in CSR and found that Ercc1(-)(/)(-) splenic B cells show moderately reduced CSR in vitro, demonstrating that ERCC1-XPF participates in, but is not required for, CSR. To investigate the role of ERCC1 in CSR, the nucleotide ...


The Mu Switch Region Tandem Repeats Are Important, But Not Required, For Antibody Class Switch Recombination, Thomas M. Luby, Carol E. Schrader, Janet Stavnezer, Erik Selsing Jan 2001

The Mu Switch Region Tandem Repeats Are Important, But Not Required, For Antibody Class Switch Recombination, Thomas M. Luby, Carol E. Schrader, Janet Stavnezer, Erik Selsing

Women’s Health Research Faculty Publications

Class switch DNA recombinations change the constant (C) region of the antibody heavy (H) chain expressed by a B cell and thereby change the antibody effector function. Unusual tandemly repeated sequence elements located upstream of H chain gene exons have long been thought to be important in the targeting and/or mechanism of the switch recombination process. We have deleted the entire switch tandem repeat element (S(mu)) from the murine (mu) H chain gene. We find that the S(mu) tandem repeats are not required for class switching in the mouse immunoglobulin H-chain locus, although the efficiency of switching ...