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Medicine and Health Sciences

University of Massachusetts Medical School

1999

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Reduced Isotype Switching In Splenic B Cells From Mice Deficient In Mismatch Repair Enzymes, Carol E. Schrader, Winifred Edelmann, R. Kucherlapati, Janet Stavnezer Aug 1999

Reduced Isotype Switching In Splenic B Cells From Mice Deficient In Mismatch Repair Enzymes, Carol E. Schrader, Winifred Edelmann, R. Kucherlapati, Janet Stavnezer

Women’s Health Research Faculty Publications

Mice deficient in various mismatch repair (MMR) enzymes were examined to determine whether this repair pathway is involved in antibody class switch recombination. Splenic B cells from mice deficient in Msh2, Mlh1, Pms2, or Mlh1 and Pms2 were stimulated in culture with lipopolysaccharide (LPS) to induce immunoglobulin (Ig)G2b and IgG3, LPS and interleukin (IL)-4 to induce IgG1, or LPS, anti-delta-dextran, IL-4, IL-5, and transforming growth factor (TGF)-beta1 to induce IgA. After 4 d in culture, cells were surface stained for IgM and non-IgM isotypes and analyzed by FACS((R)). B cells from MMR-deficient mice show a 35-75 ...